Restriction map for
pFN21AE1592
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwI AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BalI
BamHI BanI BbeI BbrPI BcgI
BciVI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I CelII Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HaeII HapII Hin1I HpaI HpaII
KasI KpnI KspI MfeI MflI
MluI MluNI MroI MscI MspA1I
MspI MunI MvnI NaeI NarI
NciI NgoMIV NheI NlaIV NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AspHI 667 GAGCAC G[AT]GC[AT]^C
AspI 7 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 161 CCCGAG C^[CT]CG[GA]G
AvaII 12 GGTCC G^G[AT]CC
BanII 1035 GGGCTC G[GA]GC[CT]^C
BbvCI 544 GCTGAGG CCTCAGC(-5/-2)
BclI 836 TGATCA T^GATCA
BsaBI 837 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 338 CTCCTC GAGGAG(10/8)
BsgI 349 GTGCAG GTGCAG(16/14)
BsiHKAI 667 GAGCAC G[AT]GC[AT]^C
BsoBI 161 CCCGAG C^[CT]CG[GA]G
BspLU11I 788 ACATGT A^CATGT
BsrDI 506 GCAATG GCAATG(2/0)
CfoI 1150 GCGC GCG^C
Cfr13I 12 GGTCC G^G[ACGT]CC
DraI 883 TTTAAA TTT^AAA
DraII 11 AGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 2 TGGCCG [CT]^GGCC[GA]
EcoO109I 11 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 47 CCATGG C^C[AT][AT]GG
FbaI 836 TGATCA T^GATCA
HgaI 255 GACGC GACGC(5/10)
HhaI 1150 GCGC GCG^C
HinP1I 1150 GCGC G^CGC
HincII 550 GTTGAC GT[CT]^[GA]AC
HindII 550 GTTGAC GT[CT]^[GA]AC
HindIII 400 AAGCTT A^AGCTT
MamI 837 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 47 CCATGG C^CATGG
NdeI 1066 CATATG CA^TATG
PflFI 7 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 11 AGGTCCT [GA]G^G[AT]CC[CT]
SapI 992 GCTCTTC GCTCTTC(1/4)
Sau96I 12 GGTCC G^G[ACGT]CC
ScaI 318 AGTACT AGT^ACT
SmlI 311 CTCAAG C^T[CT][GA]AG
SphI 202 GCATGC GCATG^C
StuI 848 AGGCCT AGG^CCT
StyI 47 CCATGG C^C[AT][AT]GG
TaqI 1119 TCGA T^CGA
TfiI 889 GATTC G^A[AT]TC
Tth111I 7 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1119 TCGA T^CGA
XcmI 846 CCAGGCCTGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AflIII 484 ACACGT A^C[GA][CT]GT
788 ACATGT
AocI 340 CCTCAGG CC^T[ACGT]AGG
377 CCTCAGG
BbsI 235 GAAGAC GAAGAC(2/6)
1052 GAAGAC
BbvI 1134 GCAGC GCAGC(8/12)
573 GCTGC
BfaI 412 CTAG C^TAG
530 CTAG
BpmI 539 CTGGAG CTGGAG(16/14)
643 CTGGAG
BpuAI 235 GAAGAC GAAGAC(2/6)
1052 GAAGAC
BsmAI 1020 GAGAC GTCTC(1/5)
705 GTCTC
BsrI 75 ACTGG ACTGG(1/-1)
538 ACTGG
Bsu36I 340 CCTCAGG CC^T[ACGT]AGG
377 CCTCAGG
Cac8I 202 GCATGC GC[ACGT]^[ACGT]GC
850 GCCTGC
DpnII 274 GATC ^GATC
837 GATC
DsaI 47 CCATGG C^C[GA][CT]GG
855 CCGTGG
Eco81I 340 CCTCAGG CC^T[ACGT]AGG
377 CCTCAGG
HaeIII 3 GGCC GG^CC
849 GGCC
MaeI 412 CTAG C^TAG
530 CTAG
MboI 274 GATC ^GATC
837 GATC
MwoI 206 GCCAAATTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
337 GCTCCTCAGGC
NdeII 274 GATC ^GATC
837 GATC
NspI 202 GCATGC [GA]CATG^[CT]
788 ACATGT
Sau3AI 274 GATC ^GATC
837 GATC
TseI 573 GCTGC G^C[AT]GC
1134 GCAGC
Tsp45I 860 GTGAC ^GT[GC]AC
1174 GTGAC
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BssKI BstF5I BstNI DdeI
EarI EcoRII Fnu4HI FokI HinfI
HphI ItaI Ksp632I MaeII MaeIII
MboII MnlI MseI MslI MvaI
NlaIII PleI RsaI ScrFI SfaNI
SfcI Tru9I Tsp509I TspRI