Restriction map for
pFN21AE6470
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccIII AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BfrI BglII BlnI
BlpI BmyI Bpu1102I BsaAI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsoBI Bsp1286I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FauI FseI FspI HaeII
HindIII HpaI KasI KpnI Ksp632I
KspI MfeI MluI MroI MunI
NarI NdeI NheI NlaIV NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI PstI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccII 43 CGCG CG^CG
AclI 718 AACGTT AA^CGTT
AcyI 15 GACGCC G[GA]^CG[CT]C
Asp700I 299 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1280 TGGCCA TGG^CCA
BclI 162 TGATCA T^GATCA
BcnI 29 CCGGG CC^[GC]GG
BfaI 52 CTAG C^TAG
BglI 28 GCCGGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1116 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 15 GACGCC G[GA]^CG[CT]C
BseRI 46 GAGGAG GAGGAG(10/8)
BsiYI 870 CCTTCCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 870 CCTTCCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 100 GGGAC GGGAC(10/14)
BsmI 1369 GCATTC GAATGC(1/-1)
Bsp1407I 183 TGTACA T^GTACA
BspHI 543 TCATGA T^CATGA
BsrFI 18 GCCGGC [GA]^CCGG[CT]
BsrGI 183 TGTACA T^GTACA
BstBI 1320 TTCGAA TT^CGAA
BstNI 555 CCTGG CC^[AT]GG
BstUI 43 CGCG CG^CG
CfoI 42 GCGC GCG^C
Cfr10I 18 GCCGGC [GA]^CCGG[CT]
DraII 70 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 1280 TGGCCA [CT]^GGCC[GA]
EcoO109I 70 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1482 GAATTC G^AATTC
EcoRII 555 CCTGG ^CC[AT]GG
FbaI 162 TGATCA T^GATCA
HgaI 15 GACGC GACGC(5/10)
HhaI 42 GCGC GCG^C
Hin1I 15 GACGCC G[GA]^CG[CT]C
HinP1I 42 GCGC G^CGC
HphI 477 GGTGA GGTGA(8/7)
MaeI 52 CTAG C^TAG
MamI 1116 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1280 TGGCCA TGG^CCA
MscI 1280 TGGCCA TGG^CCA
MspA1I 487 CAGCTG C[AC]G^C[GT]G
MvaI 555 CCTGG CC^[AT]GG
MvnI 43 CGCG CG^CG
NaeI 18 GCCGGC GCC^GGC
NciI 29 CCGGG CC^[GC]GG
NgoMIV 18 GCCGGC G^CCGGC
NspV 1320 TTCGAA TT^CGAA
Psp1406I 718 AACGTT AA^CGTT
PvuII 487 CAGCTG CAG^CTG
RcaI 543 TCATGA T^CATGA
SfuI 1320 TTCGAA TT^CGAA
SgrAI 17 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 911 CTCAAG C^T[CT][GA]AG
SspBI 183 TGTACA T^GTACA
TaqI 1321 TCGA T^CGA
TthHB8I 1321 TCGA T^CGA
XcmI 716 CCAACGTTTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 299 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BpmI 556 CTGGAG CTGGAG(16/14)
1376 CTGGAG
BssKI 29 CCGGG ^CC[ACGT]GG
555 CCTGG
BstYI 777 AGATCC [GA]^GATC[CT]
1183 AGATCC
DdeI 355 CTGAG C^T[ACGT]AG
519 CTTAG
DraI 361 TTTAAA TTT^AAA
1014 TTTAAA
DsaI 725 CCATGG C^C[GA][CT]GG
1125 CCATGG
MaeII 333 ACGT A^CGT
719 ACGT
MflI 777 AGATCC [GA]^GATC[CT]
1183 AGATCC
MslI 858 CATAGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1074 CATTTTTGTG
NcoI 725 CCATGG C^CATGG
1125 CCATGG
NspI 770 ACATGC [GA]CATG^[CT]
1489 ACATGC
PleI 414 GAGTC GAGTC(4/5)
1464 GAGTC
ScrFI 29 CCGGG CC^[ACGT]GG
555 CCTGG
SfcI 498 CTGTAG C^T[GA][CT]AG
1364 CTATAG
XhoII 777 AGATCC [GA]^GATC[CT]
1183 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbsI BbvI BpuAI
BsaJI BsrI BstF5I Cac8I Cfr13I
DpnII EcoT14I Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
ItaI MaeIII MboI MboII MnlI
MseI MspI MwoI NdeII NlaIII
RsaI Sau3AI Sau96I SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI