Restriction map for
pFN21AB0984
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# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AhdI
Alw44I AlwI AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DpnII DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoT14I EcoT22I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HpaI KasI KpnI Ksp632I KspI
MaeII MboI MfeI MflI MluI
MluNI MroI MscI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StyI
SwaI XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AciI 1123 GCGG CCGC(-3/-1)
AfaI 952 GTAC GT^AC
AgeI 362 ACCGGT A^CCGGT
ApaI 817 GGGCCC GGGCC^C
AvaI 1011 CTCGGG C^[CT]CG[GA]G
AvaII 1032 GGTCC G^G[AT]CC
BbvCI 397 GCTGAGG CCTCAGC(-5/-2)
BglI 649 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 345 CTCCAG CTGGAG(16/14)
BsaBI 1270 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 1010 CCTCGG C^C[ACGT][ACGT]GG
BsaWI 362 ACCGGT [AT]^CCGG[AT]
BsmI 160 GAATGC GAATGC(1/-1)
BsoBI 1011 CTCGGG C^[CT]CG[GA]G
BsrFI 362 ACCGGT [GA]^CCGG[CT]
BstEII 75 GGTCACC G^GT[ACGT]ACC
BstPI 75 GGTCACC G^GT[ACGT]ACC
Cfr10I 362 ACCGGT [GA]^CCGG[CT]
DraII 816 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 816 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 75 GGTCACC G^GT[ACGT]ACC
EcoRI 3 GAATTC G^AATTC
EcoRV 895 GATATC GAT^ATC
FauI 1123 GCGGG CCCGC(4/6)
HincII 171 GTCAAC GT[CT]^[GA]AC
HindII 171 GTCAAC GT[CT]^[GA]AC
MamI 1270 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PinAI 362 ACCGGT A^CCGGT
RsaI 952 GTAC GT^AC
SspI 1019 AATATT AAT^ATT
TaqI 630 TCGA T^CGA
TthHB8I 630 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AspI 70 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
374 GACACCGTC
BcnI 72 CCCGG CC^[GC]GG
408 CCGGG
BfaI 526 CTAG C^TAG
974 CTAG
BsgI 778 CTGCAC GTGCAG(16/14)
812 GTGCAG
BspHI 570 TCATGA T^CATGA
876 TCATGA
BstNI 1049 CCTGG CC^[AT]GG
1236 CCTGG
EcoRII 1049 CCTGG ^CC[AT]GG
1236 CCTGG
HindIII 415 AAGCTT A^AGCTT
449 AAGCTT
MaeI 526 CTAG C^TAG
974 CTAG
MaeIII 76 GTCAC ^GT[ACGT]AC
938 GTGAC
MslI 757 CACAGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
943 CATCAAGATG
MvaI 1049 CCTGG CC^[AT]GG
1236 CCTGG
NciI 72 CCCGG CC^[GC]GG
408 CCGGG
NlaIV 323 GGAGCC GG[ACGT]^[ACGT]CC
817 GGGCCC
NspI 782 ACATGC [GA]CATG^[CT]
923 GCATGT
PflFI 70 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
374 GACACCGTC
PflMI 383 CCATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
494 CCAGATGCTGG
RcaI 570 TCATGA T^CATGA
876 TCATGA
SacI 258 GAGCTC GAGCT^C
805 GAGCTC
StuI 106 AGGCCT AGG^CCT
1330 AGGCCT
Tsp45I 76 GTCAC ^GT[GC]AC
938 GTGAC
Tth111I 70 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
374 GACACCGTC
Van91I 383 CCATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
494 CCAGATGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AcsI AluI AlwNI ApoI Asp700I
AspHI BanII BbvI BmyI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I Cac8I
Cfr13I DdeI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MboII MnlI MseI MspI MwoI
NlaIII PleI Sau96I ScrFI SfaNI
TfiI Tru9I TseI Tsp509I TspRI
XmnI