Restriction map for
pFN21AE9555
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# NO CUT ENZYMES ( 67 enzymes) #
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AatII Acc65I AccIII AflIII AgeI
AhdI AocI AscI AseI AsnI
Asp718I AspEI AspI BcgI BsaWI
BseAI BsiWI BspDI BspEI BspLU11I
BssSI Bst1107I BstBI BstXI BstZ17I
Bsu36I ClaI DraI DraII DrdI
Eam1105I Eco81I EcoO109I EcoRI FseI
HindIII HpaI KpnI MluI MroI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI PshBI
PstI PvuI SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I StuI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 2185 GTCTAC GT^[AC][GT]AC
AclI 1447 AACGTT AA^CGTT
AflII 2625 CTTAAG C^TTAAG
Aor51HI 2812 AGCGCT AGC^GCT
Asp700I 1102 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 2012 GGATCC G^GATCC
BbrPI 2649 CACGTG CAC^GTG
BbsI 1798 GTCTTC GAAGAC(2/6)
BbvCI 2920 GCTGAGG CCTCAGC(-5/-2)
BclI 1199 TGATCA T^GATCA
BfrI 2625 CTTAAG C^TTAAG
BpuAI 1798 GTCTTC GAAGAC(2/6)
BsaAI 2649 CACGTG [CT]AC^GT[GA]
BsaI 1954 GGTCTC GGTCTC(1/5)
BsmBI 3482 CGTCTC CGTCTC(1/5)
BspMI 2341 ACCTGC ACCTGC(4/8)
BstAPI 1646 GCAAGGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI 176 CGGTCCG CG^G[AT]CCG
DraIII 2400 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2812 AGCGCT AGC^GCT
EcoNI 1835 CCTCAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 947 GATATC GAT^ATC
FbaI 1199 TGATCA T^GATCA
HincII 2587 GTCAAC GT[CT]^[GA]AC
HindII 2587 GTCAAC GT[CT]^[GA]AC
MfeI 1292 CAATTG C^AATTG
MunI 1292 CAATTG C^AATTG
NcoI 602 CCATGG C^CATGG
NdeI 2100 CATATG CA^TATG
NheI 2355 GCTAGC G^CTAGC
PflMI 1291 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2649 CACGTG CAC^GTG
PmlI 2649 CACGTG CAC^GTG
Psp1406I 1447 AACGTT AA^CGTT
RsrII 176 CGGTCCG CG^G[AT]CCG
SapI 2667 GAAGAGC GCTCTTC(1/4)
SspI 557 AATATT AAT^ATT
Van91I 1291 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1102 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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Alw44I 56 GTGCAC G^TGCAC
649 GTGCAC
ApaI 2654 GGGCCC GGGCC^C
2869 GGGCCC
ApaLI 56 GTGCAC G^TGCAC
649 GTGCAC
AvrII 2211 CCTAGG C^CTAGG
2439 CCTAGG
BciVI 2233 GGATAC GTATCC(6/5)
3473 GTATCC
BglI 2428 GCCACAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2940 GCCCGCGCGGC
BglII 1522 AGATCT A^GATCT
2036 AGATCT
BlnI 2211 CCTAGG C^CTAGG
2439 CCTAGG
BlpI 586 GCTCAGC GC^T[ACGT]AGC
2976 GCTGAGC
Bpu1102I 586 GCTCAGC GC^T[ACGT]AGC
2976 GCTGAGC
BsrBI 411 CCGCTC CCGCTC(-3/-3)
3178 CCGCTC
BsrDI 1740 CATTGC GCAATG(2/0)
1905 CATTGC
BssHII 3046 GCGCGC G^CGCGC
3281 GCGCGC
BstEII 1726 GGTGACC G^GT[ACGT]ACC
3096 GGTCACC
BstPI 1726 GGTGACC G^GT[ACGT]ACC
3096 GGTCACC
CelII 586 GCTCAGC GC^T[ACGT]AGC
2976 GCTGAGC
EcoO65I 1726 GGTGACC G^GT[ACGT]ACC
3096 GGTCACC
MseI 1474 TTAA T^TAA
2626 TTAA
NotI 2946 GCGGCCGC GC^GGCCGC
3355 GCGGCCGC
PvuII 1584 CAGCTG CAG^CTG
1745 CAGCTG
SmaI 812 CCCGGG CCC^GGG
3323 CCCGGG
SmlI 1836 CTCAAG C^T[CT][GA]AG
2625 CTTAAG
SphI 1651 GCATGC GCATG^C
2531 GCATGC
Tru9I 1474 TTAA T^TAA
2626 TTAA
XmaCI 812 CCCGGG C^CCGGG
3323 CCCGGG
XmaI 812 CCCGGG C^CCGGG
3323 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII AviII BalI BanI
BanII BbeI BbvI BcnI BfaI
BmyI BpmI BsaBI BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspHI BsrFI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DsaI EaeI EagI
EarI EclXI Eco52I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I KspI MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV NsiI NspI PleI
RcaI RsaI SacI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SspBI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII