Restriction map for
pFN21AE1970
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AlwI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspI
AvaII AviII AvrII BamHI BanII
BbsI BbvCI BcgI BciVI BglII
BlnI BpuAI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmI BspEI
BssHII Bst1107I BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CpoI DraI
DraII DrdI DsaI EagI EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoT22I FseI FspI HincII HindII
HindIII HpaI KpnI KspI MfeI
MflI MluI MroI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tth111I XbaI XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccII 101 CGCG CG^CG
AflII 889 CTTAAG C^TTAAG
AhdI 1295 GACTGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 315 GTGCAC G^TGCAC
ApaLI 315 GTGCAC G^TGCAC
AspEI 1295 GACTGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 36 GGCGCC GGCGC^C
BbrPI 1506 CACGTG CAC^GTG
BclI 564 TGATCA T^GATCA
BfaI 737 CTAG C^TAG
BfrI 889 CTTAAG C^TTAAG
BglI 344 GCCCATGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 638 GCTCAGC GC^T[ACGT]AGC
BpmI 270 CTCCAG CTGGAG(16/14)
Bpu1102I 638 GCTCAGC GC^T[ACGT]AGC
BsaAI 1506 CACGTG [CT]AC^GT[GA]
BsaBI 327 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 52 GGTCTC GGTCTC(1/5)
BsmBI 375 GAGACG CGTCTC(1/5)
Bsp1407I 1328 TGTACA T^GTACA
BspDI 727 ATCGAT AT^CGAT
BspHI 1008 TCATGA T^CATGA
BspLU11I 467 ACATGT A^CATGT
BspMI 1222 GCAGGT ACCTGC(4/8)
BsrFI 96 GCCGGC [GA]^CCGG[CT]
BsrGI 1328 TGTACA T^GTACA
BssSI 205 CACGAG CACGAG(-5/-1)
BstUI 101 CGCG CG^CG
BstXI 1523 CCAACAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 638 GCTCAGC GC^T[ACGT]AGC
Cfr10I 96 GCCGGC [GA]^CCGG[CT]
ClaI 727 ATCGAT AT^CGAT
DraIII 544 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1295 GACTGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1585 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1536 GAATTC G^AATTC
EcoT14I 644 CCAAGG C^C[AT][AT]GG
FbaI 564 TGATCA T^GATCA
KasI 36 GGCGCC G^GCGCC
MaeI 737 CTAG C^TAG
MamI 327 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 890 TTAA T^TAA
MvnI 101 CGCG CG^CG
NaeI 96 GCCGGC GCC^GGC
NarI 36 GGCGCC GG^CGCC
NgoMIV 96 GCCGGC G^CCGGC
PflMI 950 CCATTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1506 CACGTG CAC^GTG
PmlI 1506 CACGTG CAC^GTG
RcaI 1008 TCATGA T^CATGA
SapI 121 GAAGAGC GCTCTTC(1/4)
SexAI 194 ACCTGGT A^CC[AT]GGT
SfoI 36 GGCGCC GGC^GCC
SspBI 1328 TGTACA T^GTACA
SspI 787 AATATT AAT^ATT
StyI 644 CCAAGG C^C[AT][AT]GG
Tru9I 890 TTAA T^TAA
Van91I 950 CCATTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Asp700I 554 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
865 GAACCTGTTC
AspHI 315 GTGCAC G[AT]GC[AT]^C
601 GTGCTC
AvaI 30 CCCGGG C^[CT]CG[GA]G
40 CCCGGG
BalI 415 TGGCCA TGG^CCA
941 TGGCCA
BanI 36 GGCGCC G^G[CT][GA]CC
498 GGTGCC
BsiHKAI 315 GTGCAC G[AT]GC[AT]^C
601 GTGCTC
BsoBI 30 CCCGGG C^[CT]CG[GA]G
40 CCCGGG
BstAPI 75 GCAGTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
204 GCACGAGCTGC
EaeI 415 TGGCCA [CT]^GGCC[GA]
941 TGGCCA
EcoRV 658 GATATC GAT^ATC
837 GATATC
MaeIII 178 GTGAC ^GT[ACGT]AC
542 GTCAC
MluNI 415 TGGCCA TGG^CCA
941 TGGCCA
MscI 415 TGGCCA TGG^CCA
941 TGGCCA
MspA1I 232 CAGCTG C[AC]G^C[GT]G
277 CAGCTG
PleI 995 GACTC GAGTC(4/5)
423 GAGTC
PvuII 232 CAGCTG CAG^CTG
277 CAGCTG
ScaI 711 AGTACT AGT^ACT
1323 AGTACT
SmaI 30 CCCGGG CCC^GGG
40 CCCGGG
SmlI 604 CTCAAG C^T[CT][GA]AG
889 CTTAAG
TaqI 728 TCGA T^CGA
1151 TCGA
TfiI 730 GATTC G^A[AT]TC
1411 GATTC
Tsp45I 178 GTGAC ^GT[GC]AC
542 GTCAC
TthHB8I 728 TCGA T^CGA
1151 TCGA
XmaCI 30 CCCGGG C^CCGGG
40 CCCGGG
XmaI 30 CCCGGG C^CCGGG
40 CCCGGG
XmnI 554 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
865 GAACCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwNI ApoI BbvI BcnI
BmyI BsaHI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrBI BsrDI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MboI MboII MnlI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TseI Tsp509I TspRI