Restriction map for
pFN21AE1729
##################################
# NO CUT ENZYMES ( 172 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BcnI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT14I FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
KasI KspI MaeII MfeI MluI
MluNI MroI MscI MseI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TaqI Tru9I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 103 GGTACC G^GTACC
AfaI 104 GTAC GT^AC
AluI 4 AGCT AG^CT
AlwI 412 GATCC GGATC(4/5)
AocI 415 CCTGAGG CC^T[ACGT]AGG
Asp700I 569 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 103 GGTACC G^GTACC
AvaII 204 GGACC G^G[AT]CC
BanI 103 GGTACC G^G[CT][GA]CC
BanII 43 GGGCTC G[GA]GC[CT]^C
BbsI 298 GAAGAC GAAGAC(2/6)
BciVI 660 GGATAC GTATCC(6/5)
BclI 705 TGATCA T^GATCA
BglI 24 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 43 GGGCTC G[AGT]GC[ACT]^C
BpmI 553 CTGGAG CTGGAG(16/14)
BpuAI 298 GAAGAC GAAGAC(2/6)
BsaBI 700 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1286I 43 GGGCTC G[AGT]GC[ACT]^C
BstYI 411 AGATCC [GA]^GATC[CT]
Bsu36I 415 CCTGAGG CC^T[ACGT]AGG
Cac8I 33 GCCAGC GC[ACGT]^[ACGT]GC
DraII 89 AGGGCCT [GA]G^G[ACGT]CC[CT]
EarI 292 GAAGAG CTCTTC(1/4)
Eco81I 415 CCTGAGG CC^T[ACGT]AGG
EcoO109I 89 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 191 ATGCAT ATGCA^T
FauI 21 CCCGC CCCGC(4/6)
FbaI 705 TGATCA T^GATCA
HphI 243 GGTGA GGTGA(8/7)
KpnI 103 GGTACC GGTAC^C
Ksp632I 292 GAAGAG CTCTTC(1/4)
MaeIII 212 GTGAC ^GT[ACGT]AC
MamI 700 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 411 AGATCC [GA]^GATC[CT]
MspA1I 35 CAGCGG C[AC]G^C[GT]G
NsiI 191 ATGCAT ATGCA^T
NspI 189 ACATGC [GA]CATG^[CT]
RsaI 104 GTAC GT^AC
StuI 398 AGGCCT AGG^CCT
Tsp45I 212 GTGAC ^GT[GC]AC
XhoII 411 AGATCC [GA]^GATC[CT]
XmnI 569 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcsI 253 AAATTT [GA]^AATT[CT]
331 GAATTT
ApoI 253 AAATTT [GA]^AATT[CT]
331 GAATTT
BfaI 220 CTAG C^TAG
347 CTAG
BsiYI 22 CCGCCGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
34 CCAGCGGAGGG
BslI 22 CCGCCGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
34 CCAGCGGAGGG
BsmFI 203 GGGAC GGGAC(10/14)
485 GGGAC
BsrDI 370 GCAATG GCAATG(2/0)
382 GCAATG
DpnII 412 GATC ^GATC
706 GATC
MaeI 220 CTAG C^TAG
347 CTAG
MboI 412 GATC ^GATC
706 GATC
MslI 229 CATTTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
378 CACTGCAATG
NdeII 412 GATC ^GATC
706 GATC
NlaIII 190 CATG CATG^
469 CATG
Sau3AI 412 GATC ^GATC
706 GATC
SfaNI 694 GATGC GCATC(5/9)
405 GCATC
Tsp509I 254 AATT ^AATT
332 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AlwNI BbvI BsaJI BsmI
BsrI BssKI BstF5I BstNI Cfr13I
DdeI EcoRII Fnu4HI FokI HaeIII
HinfI ItaI MboII MnlI MvaI
MwoI NlaIV Sau96I ScrFI TfiI
TseI TspRI