Restriction map for
pFN21AB7576
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvrII BamHI
BbvCI BcgI BciVI BclI BfaI
BglII BlnI BlpI Bpu1102I BsaBI
BseAI BsiHKAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KpnI
MaeI MaeIII MamI MfeI MflI
MroI MslI MunI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AfaI 24 GTAC GT^AC
AflII 751 CTTAAG C^TTAAG
AflIII 355 ACGCGT A^C[GA][CT]GT
ApaI 784 GGGCCC GGGCC^C
AvaI 781 CCCGGG C^[CT]CG[GA]G
AvaII 655 GGACC G^G[AT]CC
AviII 169 TGCGCA TGC^GCA
BalI 599 TGGCCA TGG^CCA
BbrPI 540 CACGTG CAC^GTG
BbsI 837 GAAGAC GAAGAC(2/6)
BfrI 751 CTTAAG C^TTAAG
BpmI 313 CTGGAG CTGGAG(16/14)
BpuAI 837 GAAGAC GAAGAC(2/6)
BsaAI 540 CACGTG [CT]AC^GT[GA]
BsaI 331 GAGACC GGTCTC(1/5)
BsaWI 815 ACCGGA [AT]^CCGG[AT]
BsiEI 740 CGGCCG CG[GA][CT]^CG
BsiWI 23 CGTACG C^GTACG
BsmAI 331 GAGAC GTCTC(1/5)
BsmFI 654 GGGAC GGGAC(10/14)
BsoBI 781 CCCGGG C^[CT]CG[GA]G
BspMI 402 GCAGGT ACCTGC(4/8)
BsrI 639 ACTGG ACTGG(1/-1)
DpnII 378 GATC ^GATC
EagI 740 CGGCCG C^GGCCG
EarI 847 CTCTTC CTCTTC(1/4)
EclXI 740 CGGCCG C^GGCCG
Eco52I 740 CGGCCG C^GGCCG
EcoNI 384 CCTCACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 169 TGCGCA TGC^GCA
HgaI 52 GCGTC GACGC(5/10)
Ksp632I 847 CTCTTC CTCTTC(1/4)
KspI 128 CCGCGG CCGC^GG
MboI 378 GATC ^GATC
MluI 355 ACGCGT A^CGCGT
MluNI 599 TGGCCA TGG^CCA
MscI 599 TGGCCA TGG^CCA
MseI 752 TTAA T^TAA
NaeI 765 GCCGGC GCC^GGC
NcoI 44 CCATGG C^CATGG
NdeII 378 GATC ^GATC
NgoMIV 765 GCCGGC G^CCGGC
NlaIII 45 CATG CATG^
PmaCI 540 CACGTG CAC^GTG
PmlI 540 CACGTG CAC^GTG
PpuMI 654 GGGACCT [GA]G^G[AT]CC[CT]
PstI 78 CTGCAG CTGCA^G
RsaI 24 GTAC GT^AC
SacII 128 CCGCGG CCGC^GG
Sau3AI 378 GATC ^GATC
SfaNI 275 GCATC GCATC(5/9)
SfcI 78 CTGCAG C^T[GA][CT]AG
SmaI 781 CCCGGG CCC^GGG
TaqI 380 TCGA T^CGA
TfiI 526 GATTC G^A[AT]TC
Tru9I 752 TTAA T^TAA
Tsp509I 19 AATT ^AATT
TthHB8I 380 TCGA T^CGA
XmaCI 781 CCCGGG C^CCGGG
XmaI 781 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
BsrBI 676 CCGCTC CCGCTC(-3/-3)
737 GAGCGG
BsrFI 697 GCCGGT [GA]^CCGG[CT]
765 GCCGGC
BssSI 253 CACGAG CACGAG(-5/-1)
30 CTCGTG
BstF5I 242 CATCC GGATG(2/0)
276 CATCC
Cfr10I 697 GCCGGT [GA]^CCGG[CT]
765 GCCGGC
DraII 654 GGGACCT [GA]G^G[ACGT]CC[CT]
784 GGGCCCT
DsaI 44 CCATGG C^C[GA][CT]GG
128 CCGCGG
EcoO109I 654 GGGACCT [GA]G^G[ACGT]CC[CT]
784 GGGCCCT
FokI 242 CATCC GGATG(9/13)
276 CATCC
HphI 290 TCACC GGTGA(8/7)
813 TCACC
MaeII 286 ACGT A^CGT
541 ACGT
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AcyI AluI BanI
BanII BbeI BbvI BcnI BglI
BmyI BsaHI BsaJI BseRI BsgI
BsiYI BslI Bsp1286I BssHII BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI EaeI EcoRII EcoT14I FauI
Fnu4HI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII ItaI
KasI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NlaIV PleI Sau96I ScrFI SfoI
SmlI StyI TseI TspRI