Restriction map for
pFN21AE1713
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI AhdI Aor51HI ApoI
AscI AseI AsnI Asp700I AspEI
AvaI AvrII BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspHI BspMI BsrDI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I CelII ClaI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI HincII HindII HindIII
HpaI KspI MaeI MamI MfeI
MluI MroI MseI MslI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmeI PshAI PshBI Psp1406I PstI
PvuI RcaI SacI SacII SalI
SapI SbfI SexAI SfcI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tru9I Tsp509I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 296 GGTACC G^GTACC
AflIII 557 ACATGT A^C[GA][CT]GT
AlwNI 328 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 91 GGGCCC GGGCC^C
Asp718I 296 GGTACC G^GTACC
AspI 193 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 179 TGCGCA TGC^GCA
BalI 269 TGGCCA TGG^CCA
BamHI 32 GGATCC G^GATCC
BbrPI 97 CACGTG CAC^GTG
BbsI 352 GAAGAC GAAGAC(2/6)
BglI 380 GCCCAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 352 GAAGAC GAAGAC(2/6)
BsaWI 610 ACCGGA [AT]^CCGG[AT]
BspLU11I 557 ACATGT A^CATGT
BsrBI 6 GAGCGG CCGCTC(-3/-3)
BsrFI 40 GCCGGC [GA]^CCGG[CT]
BsrI 615 ACTGG ACTGG(1/-1)
Cfr10I 40 GCCGGC [GA]^CCGG[CT]
CpoI 262 CGGACCG CG^G[AT]CCG
DraIII 286 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 564 CTCTTC CTCTTC(1/4)
EcoT14I 509 CCATGG C^C[AT][AT]GG
FseI 39 GGCCGGCC GGCCGG^CC
FspI 179 TGCGCA TGC^GCA
HphI 608 TCACC GGTGA(8/7)
KpnI 296 GGTACC GGTAC^C
Ksp632I 564 CTCTTC CTCTTC(1/4)
MaeIII 607 GTCAC ^GT[ACGT]AC
MluNI 269 TGGCCA TGG^CCA
MscI 269 TGGCCA TGG^CCA
NaeI 40 GCCGGC GCC^GGC
NcoI 509 CCATGG C^CATGG
NgoMIV 40 GCCGGC G^CCGGC
NspI 557 ACATGT [GA]CATG^[CT]
PflFI 193 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 630 GAGTC GAGTC(4/5)
PmaCI 97 CACGTG CAC^GTG
PmlI 97 CACGTG CAC^GTG
PpuMI 530 AGGACCT [GA]G^G[AT]CC[CT]
RsrII 262 CGGACCG CG^G[AT]CCG
ScaI 341 AGTACT AGT^ACT
StyI 509 CCATGG C^C[AT][AT]GG
TaqI 365 TCGA T^CGA
TfiI 550 GAATC G^A[AT]TC
Tsp45I 607 GTCAC ^GT[GC]AC
TspRI 438 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 193 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 365 TCGA T^CGA
XcmI 500 CCATGCTGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
Alw44I 100 GTGCAC G^TGCAC
283 GTGCAC
AocI 526 CCTGAGG CC^T[ACGT]AGG
534 CCTCAGG
ApaLI 100 GTGCAC G^TGCAC
283 GTGCAC
AspHI 100 GTGCAC G[AT]GC[AT]^C
283 GTGCAC
BanII 91 GGGCCC G[GA]GC[CT]^C
160 GGGCTC
BbeI 65 GGCGCC GGCGC^C
310 GGCGCC
BcnI 62 CCGGG CC^[GC]GG
423 CCCGG
BsaAI 25 CACGTA [CT]AC^GT[GA]
97 CACGTG
BsaI 577 GAGACC GGTCTC(1/5)
426 GGTCTC
BsiHKAI 100 GTGCAC G[AT]GC[AT]^C
283 GTGCAC
BsmAI 577 GAGAC GTCTC(1/5)
427 GTCTC
BsmFI 514 GTCCC GGGAC(10/14)
632 GTCCC
Bsp1407I 140 TGTACA T^GTACA
458 TGTACA
BsrGI 140 TGTACA T^GTACA
458 TGTACA
BssHII 209 GCGCGC G^CGCGC
224 GCGCGC
BstF5I 123 CATCC GGATG(2/0)
183 CATCC
BstNI 36 CCTGG CC^[AT]GG
573 CCTGG
BstYI 32 GGATCC [GA]^GATC[CT]
374 AGATCC
Bsu36I 526 CCTGAGG CC^T[ACGT]AGG
534 CCTCAGG
Eco81I 526 CCTGAGG CC^T[ACGT]AGG
534 CCTCAGG
EcoRII 36 CCTGG ^CC[AT]GG
573 CCTGG
FokI 123 CATCC GGATG(9/13)
183 CATCC
HaeII 65 GGCGCC [GA]GCGC^[CT]
310 GGCGCC
HgaI 85 GACGC GACGC(5/10)
197 GCGTC
HinfI 550 GAATC G^A[ACGT]TC
630 GAGTC
KasI 65 GGCGCC G^GCGCC
310 GGCGCC
MaeII 26 ACGT A^CGT
98 ACGT
MflI 32 GGATCC [GA]^GATC[CT]
374 AGATCC
MvaI 36 CCTGG CC^[AT]GG
573 CCTGG
NarI 65 GGCGCC GG^CGCC
310 GGCGCC
NciI 62 CCGGG CC^[GC]GG
423 CCCGG
PvuII 168 CAGCTG CAG^CTG
454 CAGCTG
SfaNI 324 GATGC GCATC(5/9)
182 GCATC
SfoI 65 GGCGCC GGC^GCC
310 GGCGCC
SspBI 140 TGTACA T^GTACA
458 TGTACA
XhoII 32 GGATCC [GA]^GATC[CT]
374 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaII BanI BbvI BmyI
BpmI BsaHI BsaJI BseRI BsiYI
BslI Bsp1286I BssKI BstAPI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I FauI
Fnu4HI HaeIII HapII HhaI Hin1I
HinP1I HpaII ItaI MboI MboII
MnlI MspA1I MspI MvnI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI TseI