Restriction map for
pFN21AE9445
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BamHI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BpmI BsaBI BsaWI BseAI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI Eam1105I Eco81I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HinfI
HpaI KpnI MaeI MamI MfeI
MluI MroI MseI MunI NdeI
NheI NotI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmeI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SalI
SapI ScaI SexAI SfiI SfuI
SmlI SnaBI SpeI SphI SspBI
SspI StuI SwaI TfiI Tru9I
Tsp509I Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 868 GTCTAC GT^[AC][GT]AC
AlwNI 898 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 225 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 667 CACGTG CAC^GTG
BlpI 952 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 952 GCTGAGC GC^T[ACGT]AGC
BsaAI 667 CACGTG [CT]AC^GT[GA]
BsaI 732 GGTCTC GGTCTC(1/5)
BseRI 157 CTCCTC GAGGAG(10/8)
BsmAI 733 GTCTC GTCTC(1/5)
BspHI 137 TCATGA T^CATGA
BspMI 523 ACCTGC ACCTGC(4/8)
BstAPI 347 GCAGCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 519 GGTCACC G^GT[ACGT]ACC
BstPI 519 GGTCACC G^GT[ACGT]ACC
BstYI 797 AGATCC [GA]^GATC[CT]
CelII 952 GCTGAGC GC^T[ACGT]AGC
EcoNI 60 CCTGAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 519 GGTCACC G^GT[ACGT]ACC
KspI 558 CCGCGG CCGC^GG
MflI 797 AGATCC [GA]^GATC[CT]
MslI 996 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NruI 113 TCGCGA TCG^CGA
PmaCI 667 CACGTG CAC^GTG
PmlI 667 CACGTG CAC^GTG
PstI 579 CTGCAG CTGCA^G
RcaI 137 TCATGA T^CATGA
SacII 558 CCGCGG CCGC^GG
SbfI 578 CCTGCAGG CCTGCA^GG
SgrAI 457 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1014 CCCGGG CCC^GGG
Sse8387I 578 CCTGCAGG CCTGCA^GG
XcmI 257 CCAGCGCCGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 797 AGATCC [GA]^GATC[CT]
XmaCI 1014 CCCGGG C^CCGGG
XmaI 1014 CCCGGG C^CCGGG
XmnI 225 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 455 GGCGCC G[GA]^CG[CT]C
689 GGCGCC
AfaI 450 GTAC GT^AC
819 GTAC
AluI 707 AGCT AG^CT
956 AGCT
AlwI 798 GATCC GGATC(4/5)
767 GGATC
Aor51HI 419 AGCGCT AGC^GCT
695 AGCGCT
AvaI 814 CCCGAG C^[CT]CG[GA]G
1014 CCCGGG
BalI 254 TGGCCA TGG^CCA
725 TGGCCA
BanI 455 GGCGCC G^G[CT][GA]CC
689 GGCGCC
BbeI 455 GGCGCC GGCGC^C
689 GGCGCC
BglI 164 GCCTGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
173 GCCTGGTGGGC
BsaHI 455 GGCGCC G[GA]^CG[CT]C
689 GGCGCC
BsoBI 814 CCCGAG C^[CT]CG[GA]G
1014 CCCGGG
BsrBI 631 CCGCTC CCGCTC(-3/-3)
913 CCGCTC
BsrI 452 ACTGG ACTGG(1/-1)
773 ACTGG
EarI 277 CTCTTC CTCTTC(1/4)
781 CTCTTC
Eco47III 419 AGCGCT AGC^GCT
695 AGCGCT
EcoT14I 728 CCATGG C^C[AT][AT]GG
995 CCATGG
Hin1I 455 GGCGCC G[GA]^CG[CT]C
689 GGCGCC
HphI 521 TCACC GGTGA(8/7)
992 TCACC
KasI 455 GGCGCC G^GCGCC
689 GGCGCC
Ksp632I 277 CTCTTC CTCTTC(1/4)
781 CTCTTC
MluNI 254 TGGCCA TGG^CCA
725 TGGCCA
MscI 254 TGGCCA TGG^CCA
725 TGGCCA
NaeI 402 GCCGGC GCC^GGC
458 GCCGGC
NarI 455 GGCGCC GG^CGCC
689 GGCGCC
NcoI 728 CCATGG C^CATGG
995 CCATGG
NgoMIV 402 GCCGGC G^CCGGC
458 GCCGGC
PpuMI 359 GGGTCCT [GA]G^G[AT]CC[CT]
503 GGGTCCT
RsaI 450 GTAC GT^AC
819 GTAC
SfcI 81 CTACAG C^T[GA][CT]AG
579 CTGCAG
SfoI 455 GGCGCC GGC^GCC
689 GGCGCC
StyI 728 CCATGG C^C[AT][AT]GG
995 CCATGG
TaqI 104 TCGA T^CGA
770 TCGA
TspRI 825 CACTG CA[GC]TG[ACGT][ACGT]^
1006 CAGTG
TthHB8I 104 TCGA T^CGA
770 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI ApaI AvaII BanII
BbsI BbvI BcnI BmyI BpuAI
BsaJI BsgI BsiEI BsiYI BslI
BsmFI Bsp1286I BsrFI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EclXI Eco52I EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HpaII
ItaI MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI TseI
Tsp45I