Restriction map for
pFN21AB3260
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# NO CUT ENZYMES ( 68 enzymes) #
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AccIII AclI AcsI AflII AgeI
AhdI ApoI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BfrI BglII BlnI BseAI BsmI
BspDI BspEI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI DraI DrdI
Eam1105I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HindIII HpaI MfeI
MluI MroI MseI MunI NdeI
NheI NruI NsiI NspV PacI
PflFI PinAI PmeI PshBI Psp1406I
PvuI SexAI SfiI SfuI SnaBI
SpeI SspI SwaI Tru9I Tth111I
XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 3236 GGTACC G^GTACC
Alw44I 1711 GTGCAC G^TGCAC
AocI 1927 CCTGAGG CC^T[ACGT]AGG
Aor51HI 4238 AGCGCT AGC^GCT
ApaLI 1711 GTGCAC G^TGCAC
Asp718I 3236 GGTACC G^GTACC
BamHI 2304 GGATCC G^GATCC
BciVI 3818 GTATCC GTATCC(6/5)
BclI 1586 TGATCA T^GATCA
BsaBI 2667 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 126 CGTACG C^GTACG
Bsp1407I 4529 TGTACA T^GTACA
BspHI 2108 TCATGA T^CATGA
BsrDI 1125 CATTGC GCAATG(2/0)
BsrGI 4529 TGTACA T^GTACA
BssHII 3914 GCGCGC G^CGCGC
Bsu36I 1927 CCTGAGG CC^T[ACGT]AGG
Eco47III 4238 AGCGCT AGC^GCT
Eco81I 1927 CCTGAGG CC^T[ACGT]AGG
FbaI 1586 TGATCA T^GATCA
KpnI 3236 GGTACC GGTAC^C
KspI 86 CCGCGG CCGC^GG
MamI 2667 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 142 GCCGGC GCC^GGC
NgoMIV 142 GCCGGC G^CCGGC
NotI 193 GCGGCCGC GC^GGCCGC
PaeR7I 1978 CTCGAG C^TCGAG
RcaI 2108 TCATGA T^CATGA
SacII 86 CCGCGG CCGC^GG
SalI 1267 GTCGAC G^TCGAC
SapI 432 GCTCTTC GCTCTTC(1/4)
SbfI 1533 CCTGCAGG CCTGCA^GG
ScaI 4682 AGTACT AGT^ACT
SgrAI 141 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 1978 CTCGAG C^T[CT][GA]AG
SphI 4354 GCATGC GCATG^C
Sse8387I 1533 CCTGCAGG CCTGCA^GG
SspBI 4529 TGTACA T^GTACA
Tsp509I 4306 AATT ^AATT
XhoI 1978 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 31 enzymes) #
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AatII 1735 GACGTC GACGT^C
2077 GACGTC
ApaI 4162 GGGCCC GGGCC^C
4258 GGGCCC
BbeI 2987 GGCGCC GGCGC^C
4608 GGCGCC
BbrPI 2131 CACGTG CAC^GTG
4294 CACGTG
BbvCI 2030 CCTCAGC CCTCAGC(-5/-2)
3084 CCTCAGC
BfaI 1147 CTAG C^TAG
2621 CTAG
BsaAI 2131 CACGTG [CT]AC^GT[GA]
4294 CACGTG
BsaWI 4022 TCCGGT [AT]^CCGG[AT]
4421 ACCGGA
BsmBI 514 GAGACG CGTCTC(1/5)
1559 GAGACG
BspLU11I 491 ACATGT A^CATGT
4541 ACATGT
BsrFI 142 GCCGGC [GA]^CCGG[CT]
3325 ACCGGC
BstAPI 3741 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4349 GCATCGCATGC
Cfr10I 142 GCCGGC [GA]^CCGG[CT]
3325 ACCGGC
CpoI 18 CGGACCG CG^G[AT]CCG
1310 CGGACCG
EagI 194 CGGCCG C^GGCCG
2625 CGGCCG
EclXI 194 CGGCCG C^GGCCG
2625 CGGCCG
Eco52I 194 CGGCCG C^GGCCG
2625 CGGCCG
KasI 2987 GGCGCC G^GCGCC
4608 GGCGCC
MaeI 1147 CTAG C^TAG
2621 CTAG
NarI 2987 GGCGCC GG^CGCC
4608 GGCGCC
NcoI 842 CCATGG C^CATGG
3170 CCATGG
PmaCI 2131 CACGTG CAC^GTG
4294 CACGTG
PmlI 2131 CACGTG CAC^GTG
4294 CACGTG
PshAI 1224 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2073 GACAGACGTC
RsrII 18 CGGACCG CG^G[AT]CCG
1310 CGGACCG
SfoI 2987 GGCGCC GGC^GCC
4608 GGCGCC
SmaI 3823 CCCGGG CCC^GGG
4317 CCCGGG
StuI 1931 AGGCCT AGG^CCT
2027 AGGCCT
TfiI 2222 GAATC G^A[AT]TC
2589 GAATC
XmaCI 3823 CCCGGG C^CCGGG
4317 CCCGGG
XmaI 3823 CCCGGG C^CCGGG
4317 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcgI BcnI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TseI Tsp45I
TspRI TthHB8I Van91I XhoII