Restriction map for
pFN21AE1174
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AhdI Alw44I AlwI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AviII AvrII BamHI BcgI BciVI
BclI BfrI BglII BlnI BsaBI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrGI BssHII
Bst1107I BstAPI BstBI BstYI BstZ17I
ClaI CpoI DpnII DraI Eam1105I
Eco47III EcoRI EcoRV EcoT22I FbaI
FspI HincII HindII HindIII HpaI
KpnI MamI MboI MfeI MflI
MluI MseI MslI MunI NdeI
NdeII NheI NruI NsiI NspI
NspV PacI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI Sau3AI ScaI SexAI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI TfiI Tru9I
Tsp509I XbaI XhoII XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccIII 627 TCCGGA T^CCGGA
AfaI 1314 GTAC GT^AC
AgeI 956 ACCGGT A^CCGGT
AocI 1250 CCTCAGG CC^T[ACGT]AGG
AspI 231 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1534 CACGTG CAC^GTG
BbsI 1594 GTCTTC GAAGAC(2/6)
BbvCI 378 CCTCAGC CCTCAGC(-5/-2)
BfaI 430 CTAG C^TAG
BpuAI 1594 GTCTTC GAAGAC(2/6)
BsaAI 1534 CACGTG [CT]AC^GT[GA]
BsaI 1333 GAGACC GGTCTC(1/5)
BseAI 627 TCCGGA T^CCGGA
BspEI 627 TCCGGA T^CCGGA
BssSI 644 CTCGTG CACGAG(-5/-1)
Bsu36I 1250 CCTCAGG CC^T[ACGT]AGG
DraIII 1825 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1203 GACGCTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1891 GAAGAG CTCTTC(1/4)
Eco81I 1250 CCTCAGG CC^T[ACGT]AGG
EcoNI 468 CCTCCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 789 GGCCGGCC GGCCGG^CC
Ksp632I 1891 GAAGAG CTCTTC(1/4)
KspI 1717 CCGCGG CCGC^GG
MaeI 430 CTAG C^TAG
MroI 627 TCCGGA T^CCGGA
NcoI 1116 CCATGG C^CATGG
NotI 40 GCGGCCGC GC^GGCCGC
PaeR7I 283 CTCGAG C^TCGAG
PflFI 231 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 956 ACCGGT A^CCGGT
PmaCI 1534 CACGTG CAC^GTG
PmlI 1534 CACGTG CAC^GTG
RsaI 1314 GTAC GT^AC
SacII 1717 CCGCGG CCGC^GG
SbfI 1524 CCTGCAGG CCTGCA^GG
SfiI 784 GGCCTGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 1524 CCTGCAGG CCTGCA^GG
Tth111I 231 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 283 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwNI 70 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
181 CAGGCCCTG
BalI 29 TGGCCA TGG^CCA
614 TGGCCA
BseRI 972 CTCCTC GAGGAG(10/8)
1802 GAGGAG
BsmAI 1333 GAGAC GTCTC(1/5)
237 GTCTC
BsrDI 846 CATTGC GCAATG(2/0)
242 GCAATG
EcoT14I 1116 CCATGG C^C[AT][AT]GG
1703 CCAAGG
HinfI 82 GAGTC G^A[ACGT]TC
1210 GAGTC
MluNI 29 TGGCCA TGG^CCA
614 TGGCCA
MscI 29 TGGCCA TGG^CCA
614 TGGCCA
PleI 82 GAGTC GAGTC(4/5)
1210 GAGTC
PstI 364 CTGCAG CTGCA^G
1525 CTGCAG
PvuII 391 CAGCTG CAG^CTG
1746 CAGCTG
SfcI 364 CTGCAG C^T[GA][CT]AG
1525 CTGCAG
SmlI 283 CTCGAG C^T[CT][GA]AG
1651 CTCAAG
StuI 111 AGGCCT AGG^CCT
662 AGGCCT
StyI 1116 CCATGG C^C[AT][AT]GG
1703 CCAAGG
TaqI 284 TCGA T^CGA
419 TCGA
TthHB8I 284 TCGA T^CGA
419 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcyI AluI ApaI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BglI BlpI BmyI
BpmI Bpu1102I BsaHI BsaJI BsaWI
BsgI BsiEI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstXI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoO65I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII HphI
ItaI KasI MaeII MaeIII MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NgoMIV
NlaIII NlaIV PflMI PpuMI Sau96I
ScrFI SfaNI SfoI SmaI TseI
Tsp45I TspRI Van91I XcmI XmaCI
XmaI