Restriction map for
pFN21AE5772
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI AocI
ApaI ApoI AscI AseI AsnI
Asp700I AspEI AspI AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglII BlnI BsaBI BsaWI
BseAI BsiWI BspDI BspEI BspLU11I
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DrdI Eam1105I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI HindIII HpaI KasI MamI
MluI MroI NaeI NarI NcoI
NdeI NgoMIV NheI NruI NsiI
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SalI SapI SexAI SfiI
SfoI SgrAI SnaBI SpeI SphI
SspI SwaI Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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Acc65I 1608 GGTACC G^GTACC
AcyI 28 GACGCC G[GA]^CG[CT]C
Alw44I 1721 GTGCAC G^TGCAC
Aor51HI 123 AGCGCT AGC^GCT
ApaLI 1721 GTGCAC G^TGCAC
Asp718I 1608 GGTACC G^GTACC
AviII 75 TGCGCA TGC^GCA
BbsI 745 GTCTTC GAAGAC(2/6)
BclI 1190 TGATCA T^GATCA
BlpI 861 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 861 GCTGAGC GC^T[ACGT]AGC
BpuAI 745 GTCTTC GAAGAC(2/6)
BsaHI 28 GACGCC G[GA]^CG[CT]C
BsaI 1645 GAGACC GGTCTC(1/5)
BseRI 490 GAGGAG GAGGAG(10/8)
BsiEI 1772 CGGCCG CG[GA][CT]^CG
BsmBI 481 GAGACG CGTCTC(1/5)
BsmI 1014 GCATTC GAATGC(1/-1)
Bsp1407I 1565 TGTACA T^GTACA
BspHI 1166 TCATGA T^CATGA
BsrBI 1321 GAGCGG CCGCTC(-3/-3)
BsrFI 1136 GCCGGT [GA]^CCGG[CT]
BsrGI 1565 TGTACA T^GTACA
BstBI 60 TTCGAA TT^CGAA
BstYI 1199 AGATCC [GA]^GATC[CT]
CelII 861 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1136 GCCGGT [GA]^CCGG[CT]
DraIII 1465 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1772 CGGCCG C^GGCCG
EarI 827 GAAGAG CTCTTC(1/4)
EclXI 1772 CGGCCG C^GGCCG
Eco47III 123 AGCGCT AGC^GCT
Eco52I 1772 CGGCCG C^GGCCG
FbaI 1190 TGATCA T^GATCA
FspI 75 TGCGCA TGC^GCA
Hin1I 28 GACGCC G[GA]^CG[CT]C
HincII 1744 GTCAAC GT[CT]^[GA]AC
HindII 1744 GTCAAC GT[CT]^[GA]AC
KpnI 1608 GGTACC GGTAC^C
Ksp632I 827 GAAGAG CTCTTC(1/4)
KspI 791 CCGCGG CCGC^GG
MfeI 382 CAATTG C^AATTG
MflI 1199 AGATCC [GA]^GATC[CT]
MunI 382 CAATTG C^AATTG
NotI 1771 GCGGCCGC GC^GGCCGC
NspI 1748 ACATGC [GA]CATG^[CT]
NspV 60 TTCGAA TT^CGAA
PflMI 870 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1166 TCATGA T^CATGA
SacI 679 GAGCTC GAGCT^C
SacII 791 CCGCGG CCGC^GG
ScaI 848 AGTACT AGT^ACT
SfuI 60 TTCGAA TT^CGAA
SspBI 1565 TGTACA T^GTACA
StuI 701 AGGCCT AGG^CCT
Van91I 870 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1199 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 792 CGCG CG^CG
1393 CGCG
AlwI 1200 GATCC GGATC(4/5)
1305 GGATC
AlwNI 257 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1309 CAGACACTG
BglI 929 GCCTGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1033 GCCGTCATGGC
BsaAI 1300 TACGTG [CT]AC^GT[GA]
1382 TACGTG
BsgI 1462 CTGCAC GTGCAG(16/14)
970 GTGCAG
BsrDI 47 GCAATG GCAATG(2/0)
803 GCAATG
BstUI 792 CGCG CG^CG
1393 CGCG
DsaI 791 CCGCGG C^C[GA][CT]GG
1469 CCGTGG
EcoT14I 174 CCTTGG C^C[AT][AT]GG
1482 CCTTGG
MseI 448 TTAA T^TAA
1496 TTAA
MvnI 792 CGCG CG^CG
1393 CGCG
PaeR7I 1175 CTCGAG C^TCGAG
1411 CTCGAG
PvuII 257 CAGCTG CAG^CTG
526 CAGCTG
SbfI 1026 CCTGCAGG CCTGCA^GG
1401 CCTGCAGG
SmaI 1019 CCCGGG CCC^GGG
1592 CCCGGG
Sse8387I 1026 CCTGCAGG CCTGCA^GG
1401 CCTGCAGG
StyI 174 CCTTGG C^C[AT][AT]GG
1482 CCTTGG
Tru9I 448 TTAA T^TAA
1496 TTAA
Tsp509I 58 AATT ^AATT
383 AATT
XcmI 269 CCACCTTCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
995 CCATGAACCTCCTGG
XhoI 1175 CTCGAG C^TCGAG
1411 CTCGAG
XmaCI 1019 CCCGGG C^CCGGG
1592 CCCGGG
XmaI 1019 CCCGGG C^CCGGG
1592 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaI
AvaII BalI BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI TseI Tsp45I
TspRI TthHB8I