Restriction map for
pFN21AE0397
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# NO CUT ENZYMES ( 189 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaII AviII
AvrII BamHI BbrPI BbvCI BbvI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I Cac8I CelII
Cfr10I Cfr13I ClaI CpoI DdeI
DpnII DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI Fnu4HI
FseI FspI HincII HindII HindIII
HinfI HpaI ItaI KpnI KspI
MaeI MaeII MaeIII MamI MboI
MfeI MflI MluI MroI MseI
MslI MspA1I MunI MvnI NaeI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI Sau3AI Sau96I SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
TseI Tsp45I Tsp509I Tth111I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AluI 98 AGCT AG^CT
AlwNI 142 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 221 CCCGGG C^[CT]CG[GA]G
BalI 138 TGGCCA TGG^CCA
BanI 202 GGCGCC G^G[CT][GA]CC
BanII 37 GAGCCC G[GA]GC[CT]^C
BbeI 202 GGCGCC GGCGC^C
BbsI 166 GAAGAC GAAGAC(2/6)
BcgI 244 CGAGAGTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 37 GAGCCC G[AGT]GC[ACT]^C
BpmI 100 CTGGAG CTGGAG(16/14)
BpuAI 166 GAAGAC GAAGAC(2/6)
BsmAI 23 GAGAC GTCTC(1/5)
BsoBI 221 CCCGGG C^[CT]CG[GA]G
Bsp1286I 37 GAGCCC G[AGT]GC[ACT]^C
BstF5I 235 GGATG GGATG(2/0)
CfoI 203 GCGC GCG^C
DsaI 41 CCATGG C^C[GA][CT]GG
EaeI 138 TGGCCA [CT]^GGCC[GA]
EarI 172 CTCTTC CTCTTC(1/4)
FokI 235 GGATG GGATG(9/13)
HaeII 202 GGCGCC [GA]GCGC^[CT]
HgaI 256 GACGC GACGC(5/10)
HhaI 203 GCGC GCG^C
HinP1I 203 GCGC G^CGC
HphI 79 GGTGA GGTGA(8/7)
KasI 202 GGCGCC G^GCGCC
Ksp632I 172 CTCTTC CTCTTC(1/4)
MluNI 138 TGGCCA TGG^CCA
MscI 138 TGGCCA TGG^CCA
MwoI 230 GCTTCGGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 202 GGCGCC GG^CGCC
NcoI 41 CCATGG C^CATGG
NlaIII 42 CATG CATG^
PflMI 141 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfaNI 236 GATGC GCATC(5/9)
SfoI 202 GGCGCC GGC^GCC
SmaI 221 CCCGGG CCC^GGG
StuI 122 AGGCCT AGG^CCT
TaqI 243 TCGA T^CGA
TspRI 56 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 243 TCGA T^CGA
Van91I 141 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 221 CCCGGG C^CCGGG
XmaI 221 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AcyI 202 GGCGCC G[GA]^CG[CT]C
256 GACGCC
BsaHI 202 GGCGCC G[GA]^CG[CT]C
256 GACGCC
BsiYI 141 CCAGTTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
171 CCTCTTCCGGG
BslI 141 CCAGTTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
171 CCTCTTCCGGG
BsmFI 224 GGGAC GGGAC(10/14)
219 GTCCC
BsrI 55 CCAGT ACTGG(1/-1)
141 CCAGT
BstNI 66 CCTGG CC^[AT]GG
87 CCTGG
EcoRII 66 CCTGG ^CC[AT]GG
87 CCTGG
EcoT14I 41 CCATGG C^C[AT][AT]GG
135 CCTTGG
HaeIII 123 GGCC GG^CC
139 GGCC
HapII 177 CCGG C^CGG
222 CCGG
Hin1I 202 GGCGCC G[GA]^CG[CT]C
256 GACGCC
HpaII 177 CCGG C^CGG
222 CCGG
MboII 166 GAAGA GAAGA(8/7)
173 TCTTC
MspI 177 CCGG C^CGG
222 CCGG
MvaI 66 CCTGG CC^[AT]GG
87 CCTGG
NlaIV 62 GGAGCC GG[ACGT]^[ACGT]CC
202 GGCGCC
StyI 41 CCATGG C^C[AT][AT]GG
135 CCTTGG
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# 3 OR MORE CUT ENZYMES ( 6 enzymes) #
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BcnI BsaJI BssKI MnlI NciI
ScrFI