Restriction map for
pFN21AE5290
##################################
# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbsI BcgI BciVI BclI BfaI
BfrI BglII BlnI BpuAI BsaBI
BsaHI BsaWI BseAI BseRI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
ClaI CpoI DpnII DraI DrdI
Eam1105I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HinfI HpaI KasI KpnI
MaeI MamI MboI MfeI MflI
MluI MroI MseI MslI MunI
NaeI NarI NdeI NdeII NgoMIV
NheI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SalI Sau3AI SbfI
ScaI SexAI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI SwaI TaqI TfiI
Tru9I Tsp509I Tth111I TthHB8I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccI 607 GTCTAC GT^[AC][GT]AC
AccII 664 CGCG CG^CG
Aor51HI 558 AGCGCT AGC^GCT
BbrPI 452 CACGTG CAC^GTG
BbvCI 278 CCTCAGC CCTCAGC(-5/-2)
BsaAI 452 CACGTG [CT]AC^GT[GA]
BsaI 231 GGTCTC GGTCTC(1/5)
BsgI 676 CTGCAC GTGCAG(16/14)
BsmAI 232 GTCTC GTCTC(1/5)
BsrDI 424 CATTGC GCAATG(2/0)
BsrFI 486 ACCGGC [GA]^CCGG[CT]
BssSI 406 CTCGTG CACGAG(-5/-1)
BstF5I 303 CATCC GGATG(2/0)
BstUI 664 CGCG CG^CG
Cfr10I 486 ACCGGC [GA]^CCGG[CT]
Eco47III 558 AGCGCT AGC^GCT
EcoT14I 225 CCATGG C^C[AT][AT]GG
FokI 303 CATCC GGATG(9/13)
HaeII 558 AGCGCT [GA]GCGC^[CT]
KspI 663 CCGCGG CCGC^GG
MaeII 453 ACGT A^CGT
MaeIII 483 GTCAC ^GT[ACGT]AC
MvnI 664 CGCG CG^CG
NcoI 225 CCATGG C^CATGG
NotI 665 GCGGCCGC GC^GGCCGC
NspI 106 GCATGC [GA]CATG^[CT]
PmaCI 452 CACGTG CAC^GTG
PmlI 452 CACGTG CAC^GTG
PvuII 478 CAGCTG CAG^CTG
SacII 663 CCGCGG CCGC^GG
SfaNI 302 GCATC GCATC(5/9)
SfiI 213 GGCCACCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 106 GCATGC GCATG^C
StuI 356 AGGCCT AGG^CCT
StyI 225 CCATGG C^C[AT][AT]GG
Tsp45I 483 GTCAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwNI 85 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
261 CAGGACCTG
BcnI 178 CCCGG CC^[GC]GG
658 CCCGG
BsiEI 660 CGGCCG CG[GA][CT]^CG
666 CGGCCG
BsrI 473 ACTGG ACTGG(1/-1)
519 ACTGG
DraIII 679 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
741 CACGGCGTG
DsaI 225 CCATGG C^C[GA][CT]GG
663 CCGCGG
EagI 660 CGGCCG C^GGCCG
666 CGGCCG
EarI 289 CTCTTC CTCTTC(1/4)
190 GAAGAG
EclXI 660 CGGCCG C^GGCCG
666 CGGCCG
Eco52I 660 CGGCCG C^GGCCG
666 CGGCCG
HphI 308 TCACC GGTGA(8/7)
484 TCACC
Ksp632I 289 CTCTTC CTCTTC(1/4)
190 GAAGAG
MboII 190 GAAGA GAAGA(8/7)
290 TCTTC
MspA1I 478 CAGCTG C[AC]G^C[GT]G
663 CCGCGG
NciI 178 CCCGG CC^[GC]GG
658 CCCGG
PflMI 48 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
84 CCAGGCCCTGG
PstI 505 CTGCAG CTGCA^G
588 CTGCAG
SacI 124 GAGCTC GAGCT^C
182 GAGCTC
SapI 190 GAAGAGC GCTCTTC(1/4)
288 GCTCTTC
SfcI 505 CTGCAG C^T[GA][CT]AG
588 CTGCAG
Van91I 48 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
84 CCAGGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI AspHI AvaII BalI
BanII BbvI BglI BlpI BmyI
BpmI Bpu1102I BsaJI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BssKI BstNI
BstXI Cac8I CelII CfoI Cfr13I
DdeI DraII EaeI EcoO109I EcoRII
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII ItaI MluNI MnlI MscI
MspI MvaI MwoI NlaIII NlaIV
PpuMI Sau96I ScrFI TseI TspRI