Restriction map for
pFN21AE4056
##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlpI Bpu1102I BpuAI
BsaAI BsaBI BseAI BsgI BsiWI
BsmI Bsp1407I BspEI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstXI BstYI BstZ17I Bsu36I CelII
CpoI DraI DrdI Eam1105I EarI
Eco47III Eco81I EcoNI EcoRI EcoRV
EcoT22I FbaI FspI HgaI HindIII
HpaI KasI KpnI Ksp632I KspI
MamI MflI MluI MroI NarI
NdeI NheI NruI NsiI NspI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tth111I
Van91I XbaI XcmI XhoI XhoII
XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 707 GACGTC GACGT^C
AccI 508 GTAGAC GT^[AC][GT]AC
AcyI 707 GACGTC G[GA]^CG[CT]C
AflIII 446 ACACGT A^C[GA][CT]GT
AspHI 73 GAGCAC G[AT]GC[AT]^C
BanI 270 GGCACC G^G[CT][GA]CC
BpmI 784 CTCCAG CTGGAG(16/14)
BsaHI 707 GACGTC G[GA]^CG[CT]C
BsaI 758 GGTCTC GGTCTC(1/5)
BsaWI 662 ACCGGA [AT]^CCGG[AT]
BseRI 502 GAGGAG GAGGAG(10/8)
BsiHKAI 73 GAGCAC G[AT]GC[AT]^C
BsmBI 190 GAGACG CGTCTC(1/5)
BsmFI 813 GGGAC GGGAC(10/14)
BspDI 409 ATCGAT AT^CGAT
BspHI 899 TCATGA T^CATGA
BstBI 49 TTCGAA TT^CGAA
BstEII 30 GGTGACC G^GT[ACGT]ACC
BstPI 30 GGTGACC G^GT[ACGT]ACC
CfoI 515 GCGC GCG^C
ClaI 409 ATCGAT AT^CGAT
DraIII 586 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 30 GGTGACC G^GT[ACGT]ACC
HaeII 514 AGCGCC [GA]GCGC^[CT]
HhaI 515 GCGC GCG^C
Hin1I 707 GACGTC G[GA]^CG[CT]C
HinP1I 515 GCGC G^CGC
HinfI 621 GACTC G^A[ACGT]TC
MboII 311 TCTTC GAAGA(8/7)
MfeI 153 CAATTG C^AATTG
MseI 476 TTAA T^TAA
MslI 912 CATCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 153 CAATTG C^AATTG
NcoI 678 CCATGG C^CATGG
NotI 768 GCGGCCGC GC^GGCCGC
NspV 49 TTCGAA TT^CGAA
PleI 621 GACTC GAGTC(4/5)
RcaI 899 TCATGA T^CATGA
SfaNI 104 GCATC GCATC(5/9)
SfcI 537 CTACAG C^T[GA][CT]AG
SfuI 49 TTCGAA TT^CGAA
SmaI 383 CCCGGG CCC^GGG
StuI 672 AGGCCT AGG^CCT
Tru9I 476 TTAA T^TAA
Tsp509I 154 AATT ^AATT
XmaCI 383 CCCGGG C^CCGGG
XmaI 383 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 650 CGCG CG^CG
916 CGCG
AfaI 488 GTAC GT^AC
702 GTAC
AlwI 594 GATCC GGATC(4/5)
880 GATCC
ApaI 380 GGGCCC GGGCC^C
921 GGGCCC
AvaII 496 GGACC G^G[AT]CC
891 GGACC
AvrII 631 CCTAGG C^CTAGG
855 CCTAGG
BalI 179 TGGCCA TGG^CCA
365 TGGCCA
BlnI 631 CCTAGG C^CTAGG
855 CCTAGG
BsiEI 712 CGGCCG CG[GA][CT]^CG
769 CGGCCG
BspMI 119 ACCTGC ACCTGC(4/8)
273 ACCTGC
BsrDI 123 GCAATG GCAATG(2/0)
299 GCAATG
BstF5I 912 CATCC GGATG(2/0)
654 GGATG
BstUI 650 CGCG CG^CG
916 CGCG
DraII 545 AGGCCCC [GA]G^G[ACGT]CC[CT]
921 GGGCCCC
DsaI 338 CCGTGG C^C[GA][CT]GG
678 CCATGG
EagI 712 CGGCCG C^GGCCG
769 CGGCCG
EclXI 712 CGGCCG C^GGCCG
769 CGGCCG
Eco52I 712 CGGCCG C^GGCCG
769 CGGCCG
EcoO109I 545 AGGCCCC [GA]G^G[ACGT]CC[CT]
921 GGGCCCC
FokI 912 CATCC GGATG(9/13)
654 GGATG
FseI 571 GGCCGGCC GGCCGG^CC
713 GGCCGGCC
HincII 61 GTCAAC GT[CT]^[GA]AC
373 GTCAAC
HindII 61 GTCAAC GT[CT]^[GA]AC
373 GTCAAC
HphI 30 GGTGA GGTGA(8/7)
420 GGTGA
MaeIII 31 GTGAC ^GT[ACGT]AC
689 GTGAC
MluNI 179 TGGCCA TGG^CCA
365 TGGCCA
MscI 179 TGGCCA TGG^CCA
365 TGGCCA
MvnI 650 CGCG CG^CG
916 CGCG
PvuII 294 CAGCTG CAG^CTG
869 CAGCTG
RsaI 488 GTAC GT^AC
702 GTAC
TaqI 50 TCGA T^CGA
410 TCGA
Tsp45I 31 GTGAC ^GT[GC]AC
689 GTGAC
TthHB8I 50 TCGA T^CGA
410 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AvaI BanII BbvI
BcnI BfaI BmyI BsaJI BsiYI
BslI BsmAI BsoBI Bsp1286I BsrFI
BsrI BssKI BstNI Cac8I Cfr10I
Cfr13I DdeI DpnII EaeI EcoRII
EcoT14I FauI Fnu4HI HaeIII HapII
HpaII ItaI MaeI MaeII MboI
MnlI MspA1I MspI MvaI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV Sau3AI Sau96I ScrFI StyI
TseI TspRI