Restriction map for
pFN21AE6616
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BbeI
BbrPI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI DrdI Eam1105I
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
HphI KasI KpnI KspI MaeI
MamI MluI MluNI MroI MscI
MseI NarI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tth111I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 568 GTCTAC GT^[AC][GT]AC
AccII 66 CGCG CG^CG
AflIII 20 ACGTGT A^C[GA][CT]GT
AlwNI 292 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 685 CCTCAGG CC^T[ACGT]AGG
ApaI 681 GGGCCC GGGCC^C
BamHI 867 GGATCC G^GATCC
BanI 306 GGTGCC G^G[CT][GA]CC
BbsI 848 GTCTTC GAAGAC(2/6)
BbvCI 340 GCTGAGG CCTCAGC(-5/-2)
BpuAI 848 GTCTTC GAAGAC(2/6)
BsaI 360 GGTCTC GGTCTC(1/5)
BsgI 900 CTGCAC GTGCAG(16/14)
BsrBI 821 CCGCTC CCGCTC(-3/-3)
BsrFI 52 GCCGGC [GA]^CCGG[CT]
BstAPI 318 GCATTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 66 CGCG CG^CG
Bsu36I 685 CCTCAGG CC^T[ACGT]AGG
Cfr10I 52 GCCGGC [GA]^CCGG[CT]
EagI 699 CGGCCG C^GGCCG
EarI 391 CTCTTC CTCTTC(1/4)
EclXI 699 CGGCCG C^GGCCG
Eco52I 699 CGGCCG C^GGCCG
Eco81I 685 CCTCAGG CC^T[ACGT]AGG
EcoNI 248 CCTATGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Ksp632I 391 CTCTTC CTCTTC(1/4)
MaeII 20 ACGT A^CGT
MfeI 995 CAATTG C^AATTG
MslI 460 CATCCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 995 CAATTG C^AATTG
MvnI 66 CGCG CG^CG
NaeI 52 GCCGGC GCC^GGC
NgoMIV 52 GCCGGC G^CCGGC
PflMI 858 CCACCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 563 GAGTC GAGTC(4/5)
PstI 964 CTGCAG CTGCA^G
PvuI 47 CGATCG CGAT^CG
SexAI 401 ACCTGGT A^CC[AT]GGT
SmaI 827 CCCGGG CCC^GGG
Tsp45I 92 GTGAC ^GT[GC]AC
Van91I 858 CCACCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 827 CCCGGG C^CCGGG
XmaI 827 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
BglI 648 GCCTGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
732 GCCCAGCAGGC
BpmI 838 CTGGAG CTGGAG(16/14)
880 CTGGAG
BsiEI 47 CGATCG CG[GA][CT]^CG
699 CGGCCG
BsmAI 361 GTCTC GTCTC(1/5)
637 GTCTC
BsrI 497 ACTGG ACTGG(1/-1)
912 ACTGG
BstF5I 460 CATCC GGATG(2/0)
336 GGATG
BstXI 350 CCACCCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
872 CCAAGGACCTGG
BstYI 141 GGATCT [GA]^GATC[CT]
867 GGATCC
CfoI 67 GCGC GCG^C
621 GCGC
DraIII 400 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
859 CACCTTGTG
EcoT14I 558 CCAAGG C^C[AT][AT]GG
872 CCAAGG
FauI 354 CCCGC CCCGC(4/6)
111 GCGGG
FokI 460 CATCC GGATG(9/13)
336 GGATG
HhaI 67 GCGC GCG^C
621 GCGC
HinP1I 67 GCGC G^CGC
621 GCGC
MaeIII 92 GTGAC ^GT[ACGT]AC
238 GTTAC
MflI 141 GGATCT [GA]^GATC[CT]
867 GGATCC
NlaIII 89 CATG CATG^
725 CATG
PvuII 157 CAGCTG CAG^CTG
961 CAGCTG
SfaNI 337 GATGC GCATC(5/9)
618 GATGC
SfcI 594 CTACAG C^T[GA][CT]AG
964 CTGCAG
StyI 558 CCAAGG C^C[AT][AT]GG
872 CCAAGG
TfiI 674 GATTC G^A[AT]TC
814 GATTC
Tsp509I 809 AATT ^AATT
996 AATT
TspRI 296 CACTG CA[GC]TG[ACGT][ACGT]^
641 CAGTG
XhoII 141 GGATCT [GA]^GATC[CT]
867 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
AvaII BanII BbvI BcnI BmyI
BsaJI BseRI BsiYI BslI BsmFI
BsoBI Bsp1286I BssKI BstNI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII Fnu4HI HaeIII
HapII HinfI HpaII ItaI MboI
MboII MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIV PpuMI
RsaI Sau3AI Sau96I ScrFI TaqI
TseI TthHB8I