##################################
# NO CUT ENZYMES (  209 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspHI       BspLU11I    BspMI       
BsrBI       BsrFI       BsrGI       BsrI        BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FokI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HinfI       
HpaI        KasI        KpnI        KspI        MaeI        
MaeII       MamI        MfeI        MflI        MluI        
MluNI       MscI        MslI        MspA1I      MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIII      NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        StyI        
SwaI        TaqI        TfiI        TspRI       Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AccIII        156  TCCGGA               T^CCGGA
AciI          232  GCGG                 CCGC(-3/-1)
AcsI          142  AAATTT               [GA]^AATT[CT]
AlwI          198  GGATC                GGATC(4/5)
ApoI          142  AAATTT               [GA]^AATT[CT]
BsaWI         156  TCCGGA               [AT]^CCGG[AT]
BseAI         156  TCCGGA               T^CCGGA
BsiYI         225  CCTCATTGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          225  CCTCATTGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI          17  GTCTC                GTCTC(1/5)
BsmFI         207  GTCCC                GGGAC(10/14)
BspEI         156  TCCGGA               T^CCGGA
BsrDI         228  CATTGC               GCAATG(2/0)
Cac8I          33  GCTGGC               GC[ACGT]^[ACGT]GC
Cfr13I         36  GGCCC                G^G[ACGT]CC
DdeI           86  CTGAG                C^T[ACGT]AG
DpnII         199  GATC                 ^GATC
EarI          186  GAAGAG               CTCTTC(1/4)
HapII         157  CCGG                 C^CGG
HpaII         157  CCGG                 C^CGG
HphI          243  GGTGA                GGTGA(8/7)
Ksp632I       186  GAAGAG               CTCTTC(1/4)
MaeIII         28  GTCAC                ^GT[ACGT]AC
MboI          199  GATC                 ^GATC
MroI          156  TCCGGA               T^CCGGA
MseI          130  TTAA                 T^TAA
MspI          157  CCGG                 C^CGG
NdeII         199  GATC                 ^GATC
Sau3AI        199  GATC                 ^GATC
Sau96I         36  GGCCC                G^G[ACGT]CC
SspI          249  AATATT               AAT^ATT
Tru9I         130  TTAA                 T^TAA
Tsp45I         28  GTCAC                ^GT[GC]AC
Tsp509I       143  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

BbvI          169  GCAGC                GCAGC(8/12)
               44  GCTGC               
BssKI          51  CCAGG                ^CC[ACGT]GG
              240  CCTGG               
BstNI          51  CCAGG                CC^[AT]GG
              240  CCTGG               
EcoRII         51  CCAGG                ^CC[AT]GG
              240  CCTGG               
Fnu4HI         44  GCTGC                GC^[ACGT]GC
              169  GCAGC               
HaeIII         36  GGCC                 GG^CC
               54  GGCC                
ItaI           44  GCTGC                GC^[ACGT]GC
              169  GCAGC               
MvaI           51  CCAGG                CC^[AT]GG
              240  CCTGG               
MwoI           24  GCTGGTCACGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              163  GCTGTTGCAGC         
ScrFI          51  CCAGG                CC^[ACGT]GG
              240  CCTGG               
SfaNI         148  GATGC                GCATC(5/9)
              191  GATGC               
TseI           44  GCTGC                G^C[AT]GC
              169  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (    3 enzymes) #
#########################################

AluI        MboII       MnlI