Restriction map for
pFN21AE6611
##################################
# NO CUT ENZYMES ( 181 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AfaI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BclI BfrI
BglI BglII BlnI BpmI BsaAI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DraII DraIII
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HindIII HpaI KasI
KpnI Ksp632I KspI MaeII MfeI
MflI MluI MroI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TspRI Tth111I TthHB8I
XbaI XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AlwI 279 GATCC GGATC(4/5)
AlwNI 94 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 567 TGGCCA TGG^CCA
BbsI 750 GTCTTC GAAGAC(2/6)
BciVI 388 GGATAC GTATCC(6/5)
BpuAI 750 GTCTTC GAAGAC(2/6)
BsaBI 279 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 761 CTCCTC GAGGAG(10/8)
BsrI 578 ACTGG ACTGG(1/-1)
Cfr13I 581 GGGCC G^G[ACGT]CC
DraI 250 TTTAAA TTT^AAA
DrdI 741 GACACCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 567 TGGCCA [CT]^GGCC[GA]
EcoT14I 449 CCAAGG C^C[AT][AT]GG
HgaI 748 GCGTC GACGC(5/10)
HincII 188 GTCAAC GT[CT]^[GA]AC
HindII 188 GTCAAC GT[CT]^[GA]AC
MaeIII 739 GTGAC ^GT[ACGT]AC
MamI 279 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 567 TGGCCA TGG^CCA
MscI 567 TGGCCA TGG^CCA
NheI 181 GCTAGC G^CTAGC
NlaIV 78 GGCTCC GG[ACGT]^[ACGT]CC
PflMI 282 CCAGATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Sau96I 581 GGGCC G^G[ACGT]CC
StyI 449 CCAAGG C^C[AT][AT]GG
Tsp45I 739 GTGAC ^GT[GC]AC
Tsp509I 318 AATT ^AATT
Van91I 282 CCAGATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 282 CCAGATCATGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AspHI 134 GTGCTC G[AT]GC[AT]^C
143 GTGCTC
BcnI 169 CCGGG CC^[GC]GG
193 CCGGG
BfaI 182 CTAG C^TAG
271 CTAG
BlpI 136 GCTCAGC GC^T[ACGT]AGC
145 GCTCAGC
BmyI 134 GTGCTC G[AGT]GC[ACT]^C
143 GTGCTC
Bpu1102I 136 GCTCAGC GC^T[ACGT]AGC
145 GCTCAGC
BsaJI 449 CCAAGG C^C[ACGT][ACGT]GG
483 CCTGGG
BsiHKAI 134 GTGCTC G[AT]GC[AT]^C
143 GTGCTC
Bsp1286I 134 GTGCTC G[AGT]GC[ACT]^C
143 GTGCTC
BstF5I 8 CATCC GGATG(2/0)
446 CATCC
CelII 136 GCTCAGC GC^T[ACGT]AGC
145 GCTCAGC
FokI 8 CATCC GGATG(9/13)
446 CATCC
HapII 169 CCGG C^CGG
193 CCGG
HinfI 173 GAATC G^A[ACGT]TC
528 GATTC
HpaII 169 CCGG C^CGG
193 CCGG
HphI 276 GGTGA GGTGA(8/7)
471 GGTGA
MaeI 182 CTAG C^TAG
271 CTAG
MseI 251 TTAA T^TAA
331 TTAA
MslI 519 CATTGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
612 CACGCTGATG
MspI 169 CCGG C^CGG
193 CCGG
NciI 169 CCGGG CC^[GC]GG
193 CCGGG
NlaIII 25 CATG CATG^
288 CATG
TfiI 173 GAATC G^A[AT]TC
528 GATTC
Tru9I 251 TTAA T^TAA
331 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AluI BbvI BsiYI BslI BssKI
BstNI Cac8I DdeI DpnII EcoRII
Fnu4HI HaeIII ItaI MboI MboII
MnlI MvaI MwoI NdeII Sau3AI
ScrFI SfaNI TseI