Restriction map for
pFN21AE6193
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# NO CUT ENZYMES ( 182 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BseAI
BsiEI BsiWI BsmBI BsmFI BsmI
BsoBI BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HpaI HphI
KasI KpnI KspI MaeI MaeII
MaeIII MamI MfeI MluI MluNI
MroI MscI MseI MslI MunI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TaqI
TfiI Tru9I Tsp45I Tsp509I Tth111I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AlwNI 78 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 14 GGGCCC GGGCC^C
AvaII 153 GGACC G^G[AT]CC
BamHI 3 GGATCC G^GATCC
BpmI 37 CTGGAG CTGGAG(16/14)
BsaJI 131 CCAAGG C^C[ACGT][ACGT]GG
BsaWI 89 ACCGGA [AT]^CCGG[AT]
BseRI 104 CTCCTC GAGGAG(10/8)
BsgI 247 GTGCAG GTGCAG(16/14)
BsmAI 163 GTCTC GTCTC(1/5)
Bsp1407I 62 TGTACA T^GTACA
BsrBI 187 GAGCGG CCGCTC(-3/-3)
BsrGI 62 TGTACA T^GTACA
BsrI 77 CCAGT ACTGG(1/-1)
BstYI 3 GGATCC [GA]^GATC[CT]
DpnII 4 GATC ^GATC
DraII 13 AGGGCCC [GA]G^G[ACGT]CC[CT]
EaeI 53 TGGCCG [CT]^GGCC[GA]
EcoO109I 13 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 131 CCAAGG C^C[AT][AT]GG
HapII 90 CCGG C^CGG
HincII 204 GTTGAC GT[CT]^[GA]AC
HindII 204 GTTGAC GT[CT]^[GA]AC
HinfI 139 GAGTC G^A[ACGT]TC
HpaII 90 CCGG C^CGG
MboI 4 GATC ^GATC
MflI 3 GGATCC [GA]^GATC[CT]
MspA1I 22 CAGCTG C[AC]G^C[GT]G
MspI 90 CCGG C^CGG
NdeII 4 GATC ^GATC
NlaIII 184 CATG CATG^
PflMI 18 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 139 GAGTC GAGTC(4/5)
PvuII 22 CAGCTG CAG^CTG
SapI 229 GAAGAGC GCTCTTC(1/4)
Sau3AI 4 GATC ^GATC
SspBI 62 TGTACA T^GTACA
StyI 131 CCAAGG C^C[AT][AT]GG
TspRI 78 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 18 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 3 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 31 GCGG CCGC(-3/-1)
189 GCGG
AfaI 63 GTAC GT^AC
171 GTAC
AlwI 4 GATCC GGATC(4/5)
3 GGATC
AspHI 124 GAGCTC G[AT]GC[AT]^C
255 GAGCTC
BbvI 249 GCAGC GCAGC(8/12)
28 GCTGC
BsiHKAI 124 GAGCTC G[AT]GC[AT]^C
255 GAGCTC
BsiYI 18 CCAACAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
83 CCTGCCACCGG
BslI 18 CCAACAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
83 CCTGCCACCGG
BssKI 150 CCTGG ^CC[ACGT]GG
174 CCTGG
BstNI 150 CCTGG CC^[AT]GG
174 CCTGG
DdeI 10 CTCAG C^T[ACGT]AG
167 CTAAG
DrdI 154 GACCGATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
207 GACAGAGTTGTC
EarI 229 GAAGAG CTCTTC(1/4)
240 GAAGAG
EcoRII 150 CCTGG ^CC[AT]GG
174 CCTGG
Fnu4HI 28 GCTGC GC^[ACGT]GC
249 GCAGC
ItaI 28 GCTGC GC^[ACGT]GC
249 GCAGC
Ksp632I 229 GAAGAG CTCTTC(1/4)
240 GAAGAG
MboII 229 GAAGA GAAGA(8/7)
240 GAAGA
MvaI 150 CCTGG CC^[AT]GG
174 CCTGG
NlaIV 3 GGATCC GG[ACGT]^[ACGT]CC
14 GGGCCC
RsaI 63 GTAC GT^AC
171 GTAC
SacI 124 GAGCTC GAGCT^C
255 GAGCTC
ScrFI 150 CCTGG CC^[ACGT]GG
174 CCTGG
TseI 28 GCTGC G^C[AT]GC
249 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
AluI BanII BmyI Bsp1286I BstF5I
Cac8I Cfr13I FokI HaeIII MnlI
Sau96I