Restriction map for
pFN21AE6488
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcsI AflII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BbeI BbvCI BciVI BfaI
BfrI BglII BlnI BsaBI BsaI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV EcoT22I
FauI FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshBI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I Van91I
XbaI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AclI 447 AACGTT AA^CGTT
AcyI 1156 GACGCC G[GA]^CG[CT]C
AflIII 473 ACATGT A^C[GA][CT]GT
AlwNI 330 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 673 CCTGAGG CC^T[ACGT]AGG
ApaI 1638 GGGCCC GGGCC^C
AspHI 256 GTGCTC G[AT]GC[AT]^C
AvaI 374 CCCGGG C^[CT]CG[GA]G
BamHI 284 GGATCC G^GATCC
BanI 847 GGCACC G^G[CT][GA]CC
BbrPI 144 CACGTG CAC^GTG
BbsI 1072 GTCTTC GAAGAC(2/6)
BcgI 1172 GCACTGTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 110 TGATCA T^GATCA
BpuAI 1072 GTCTTC GAAGAC(2/6)
BsaAI 144 CACGTG [CT]AC^GT[GA]
BsaHI 1156 GACGCC G[GA]^CG[CT]C
BsaWI 26 ACCGGA [AT]^CCGG[AT]
BsiHKAI 256 GTGCTC G[AT]GC[AT]^C
BsoBI 374 CCCGGG C^[CT]CG[GA]G
BspHI 509 TCATGA T^CATGA
BspLU11I 473 ACATGT A^CATGT
BsrFI 571 GCCGGT [GA]^CCGG[CT]
BstAPI 329 GCAGTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 673 CCTGAGG CC^T[ACGT]AGG
Cfr10I 571 GCCGGT [GA]^CCGG[CT]
DraIII 727 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 673 CCTGAGG CC^T[ACGT]AGG
FbaI 110 TGATCA T^GATCA
Hin1I 1156 GACGCC G[GA]^CG[CT]C
MseI 499 TTAA T^TAA
NspI 473 ACATGT [GA]CATG^[CT]
PleI 1392 GAGTC GAGTC(4/5)
PmaCI 144 CACGTG CAC^GTG
PmlI 144 CACGTG CAC^GTG
PpuMI 1506 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 1341 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 447 AACGTT AA^CGTT
RcaI 509 TCATGA T^CATGA
SexAI 601 ACCTGGT A^CC[AT]GGT
SmaI 374 CCCGGG CCC^GGG
StuI 1440 AGGCCT AGG^CCT
Tru9I 499 TTAA T^TAA
Tsp509I 685 AATT ^AATT
XmaCI 374 CCCGGG C^CCGGG
XmaI 374 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
BalI 196 TGGCCA TGG^CCA
1005 TGGCCA
BglI 46 GCCTGCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
571 GCCGGTGTGGC
BseRI 922 CTCCTC GAGGAG(10/8)
1065 CTCCTC
BstEII 739 GGTGACC G^GT[ACGT]ACC
1668 GGTGACC
BstPI 739 GGTGACC G^GT[ACGT]ACC
1668 GGTGACC
BstXI 494 CCACGTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1628 CCAATGGGATGG
BstYI 284 GGATCC [GA]^GATC[CT]
1235 AGATCC
DraII 791 AGGCCCC [GA]G^G[ACGT]CC[CT]
1506 GGGACCC
DsaI 767 CCATGG C^C[GA][CT]GG
929 CCATGG
EaeI 196 TGGCCA [CT]^GGCC[GA]
1005 TGGCCA
EcoO109I 791 AGGCCCC [GA]G^G[ACGT]CC[CT]
1506 GGGACCC
EcoO65I 739 GGTGACC G^GT[ACGT]ACC
1668 GGTGACC
EcoT14I 767 CCATGG C^C[AT][AT]GG
929 CCATGG
MflI 284 GGATCC [GA]^GATC[CT]
1235 AGATCC
MluNI 196 TGGCCA TGG^CCA
1005 TGGCCA
MscI 196 TGGCCA TGG^CCA
1005 TGGCCA
NcoI 767 CCATGG C^CATGG
929 CCATGG
PstI 1403 CTGCAG CTGCA^G
1444 CTGCAG
SfcI 1403 CTGCAG C^T[GA][CT]AG
1444 CTGCAG
StyI 767 CCATGG C^C[AT][AT]GG
929 CCATGG
TaqI 656 TCGA T^CGA
1365 TCGA
TthHB8I 656 TCGA T^CGA
1365 TCGA
XcmI 269 CCACCCTGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1377 CCACTCAGCCTGTGG
XhoII 284 GGATCC [GA]^GATC[CT]
1235 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AfaI AluI AlwI Asp700I
AvaII BanII BbvI BcnI BlpI
BmyI BpmI Bpu1102I BsaJI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I CelII CfoI Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI TfiI TseI Tsp45I TspRI
XmnI