Restriction map for
pFN21AE2795
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI AhdI Alw44I ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvaI AviII
AvrII BalI BbeI BbrPI BbvCI
BciVI BfaI BfrI BglII BlnI
BlpI BpmI Bpu1102I BsaBI BsgI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspLU11I BsrBI BsrDI BsrFI
BsrGI BstAPI BstBI CelII Cfr10I
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco52I EcoRI EcoRV
EcoT22I FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MluNI
MscI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tsp509I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 925 GTATAC GT^[AC][GT]AC
AcyI 243 GACGCC G[GA]^CG[CT]C
AlwNI 91 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 250 CCTCAGG CC^T[ACGT]AGG
Aor51HI 374 AGCGCT AGC^GCT
AspHI 427 GTGCTC G[AT]GC[AT]^C
AspI 658 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 392 GGATCC G^GATCC
BanII 195 GGGCTC G[GA]GC[CT]^C
BbsI 600 GAAGAC GAAGAC(2/6)
BclI 98 TGATCA T^GATCA
BglI 688 GCCAAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 600 GAAGAC GAAGAC(2/6)
BsaAI 238 TACGTG [CT]AC^GT[GA]
BsaHI 243 GACGCC G[GA]^CG[CT]C
BsaI 31 GAGACC GGTCTC(1/5)
BsiHKAI 427 GTGCTC G[AT]GC[AT]^C
BsmI 933 GAATGC GAATGC(1/-1)
BspHI 491 TCATGA T^CATGA
BspMI 604 ACCTGC ACCTGC(4/8)
BssHII 697 GCGCGC G^CGCGC
BssSI 133 CACGAG CACGAG(-5/-1)
Bst1107I 925 GTATAC GTA^TAC
BstEII 180 GGTAACC G^GT[ACGT]ACC
BstPI 180 GGTAACC G^GT[ACGT]ACC
BstZ17I 925 GTATAC GTA^TAC
Bsu36I 250 CCTCAGG CC^T[ACGT]AGG
DrdI 33 GACCACCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 461 CCACGG C^C[GA][CT]GG
EaeI 402 TGGCCG [CT]^GGCC[GA]
Eco47III 374 AGCGCT AGC^GCT
Eco81I 250 CCTCAGG CC^T[ACGT]AGG
EcoNI 888 CCTCCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 180 GGTAACC G^GT[ACGT]ACC
EcoT14I 185 CCAAGG C^C[AT][AT]GG
FbaI 98 TGATCA T^GATCA
HaeII 374 AGCGCT [GA]GCGC^[CT]
HgaI 243 GACGC GACGC(5/10)
Hin1I 243 GACGCC G[GA]^CG[CT]C
MseI 722 TTAA T^TAA
PflFI 658 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 626 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 491 TCATGA T^CATGA
SapI 54 GAAGAGC GCTCTTC(1/4)
ScaI 851 AGTACT AGT^ACT
SexAI 146 ACCAGGT A^CC[AT]GGT
SfcI 417 CTACAG C^T[GA][CT]AG
SmlI 1039 CTCAAG C^T[CT][GA]AG
StuI 752 AGGCCT AGG^CCT
StyI 185 CCAAGG C^C[AT][AT]GG
Tru9I 722 TTAA T^TAA
Tth111I 658 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 698 CGCG CG^CG
1104 CGCG
AccIII 199 TCCGGA T^CCGGA
431 TCCGGA
BanI 256 GGCACC G^G[CT][GA]CC
455 GGTGCC
BbvI 815 GCAGC GCAGC(8/12)
407 GCTGC
BcgI 719 GCATTAAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
905 GCATCCGCTTCG
BcnI 618 CCCGG CC^[GC]GG
788 CCCGG
BsaWI 199 TCCGGA [AT]^CCGG[AT]
431 TCCGGA
BseAI 199 TCCGGA T^CCGGA
431 TCCGGA
BseRI 331 GAGGAG GAGGAG(10/8)
1075 GAGGAG
BsmAI 31 GAGAC GTCTC(1/5)
1087 GAGAC
BspEI 199 TCCGGA T^CCGGA
431 TCCGGA
BstUI 698 CGCG CG^CG
1104 CGCG
BstYI 392 GGATCC [GA]^GATC[CT]
443 AGATCC
DraII 626 AGGACCC [GA]G^G[ACGT]CC[CT]
1171 GGGGCCT
EarI 547 CTCTTC CTCTTC(1/4)
54 GAAGAG
EcoO109I 626 AGGACCC [GA]G^G[ACGT]CC[CT]
1171 GGGGCCT
Ksp632I 547 CTCTTC CTCTTC(1/4)
54 GAAGAG
MflI 392 GGATCC [GA]^GATC[CT]
443 AGATCC
MroI 199 TCCGGA T^CCGGA
431 TCCGGA
MvnI 698 CGCG CG^CG
1104 CGCG
MwoI 688 GCCAAACTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
731 GCTGGGTGTGC
NciI 618 CCCGG CC^[GC]GG
788 CCCGG
TaqI 794 TCGA T^CGA
914 TCGA
TfiI 314 GATTC G^A[AT]TC
1165 GATTC
TseI 407 GCTGC G^C[AT]GC
815 GCAGC
Tsp45I 241 GTGAC ^GT[GC]AC
1016 GTGAC
TthHB8I 794 TCGA T^CGA
914 TCGA
XcmI 35 CCACCAAGTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
212 CCAACAGAGTCATGG
XhoII 392 GGATCC [GA]^GATC[CT]
443 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI TspRI