Restriction map for
pFN21AE6617
##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvaI AviII
AvrII BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BpuAI BsaBI BsaWI BseAI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BsrFI BssHII
Bst1107I BstBI BstUI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
KpnI KspI MaeI MamI MfeI
MluI MroI MseI MunI MvnI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tru9I Tsp509I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AcyI 824 GGCGCC G[GA]^CG[CT]C
AspHI 569 GAGCTC G[AT]GC[AT]^C
AspI 922 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 830 GGACC G^G[AT]CC
BalI 353 TGGCCA TGG^CCA
BanI 824 GGCGCC G^G[CT][GA]CC
BanII 569 GAGCTC G[GA]GC[CT]^C
BbeI 824 GGCGCC GGCGC^C
BmyI 569 GAGCTC G[AGT]GC[ACT]^C
BsaHI 824 GGCGCC G[GA]^CG[CT]C
BseRI 610 GAGGAG GAGGAG(10/8)
BsiHKAI 569 GAGCTC G[AT]GC[AT]^C
Bsp1286I 569 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 668 TGTACA T^GTACA
BsrDI 672 CATTGC GCAATG(2/0)
BsrGI 668 TGTACA T^GTACA
BstAPI 237 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 463 GGTGACC G^GT[ACGT]ACC
BstPI 463 GGTGACC G^GT[ACGT]ACC
DraII 791 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 62 CCACGG C^C[GA][CT]GG
EarI 898 CTCTTC CTCTTC(1/4)
EcoNI 192 CCTACGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 791 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 463 GGTGACC G^GT[ACGT]ACC
HaeII 824 GGCGCC [GA]GCGC^[CT]
Hin1I 824 GGCGCC G[GA]^CG[CT]C
KasI 824 GGCGCC G^GCGCC
Ksp632I 898 CTCTTC CTCTTC(1/4)
MboII 899 TCTTC GAAGA(8/7)
MluNI 353 TGGCCA TGG^CCA
MscI 353 TGGCCA TGG^CCA
MslI 285 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 824 GGCGCC GG^CGCC
PflFI 922 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 45 GACTC GAGTC(4/5)
PstI 643 CTGCAG CTGCA^G
SacI 569 GAGCTC GAGCT^C
SfiI 57 GGCCGCCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 824 GGCGCC GGC^GCC
SspBI 668 TGTACA T^GTACA
TaqI 114 TCGA T^CGA
Tth111I 922 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 114 TCGA T^CGA
XcmI 62 CCACGGCCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
BamHI 188 GGATCC G^GATCC
893 GGATCC
BglI 58 GCCGCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
972 GCCCACCTGGC
BlpI 255 GCTGAGC GC^T[ACGT]AGC
696 GCTCAGC
Bpu1102I 255 GCTGAGC GC^T[ACGT]AGC
696 GCTCAGC
BsaAI 40 TACGTG [CT]AC^GT[GA]
496 TACGTG
BsaI 439 GAGACC GGTCTC(1/5)
649 GAGACC
BsgI 550 CTGCAC GTGCAG(16/14)
688 CTGCAC
BsrBI 955 CCGCTC CCGCTC(-3/-3)
808 GAGCGG
BssSI 130 CACGAG CACGAG(-5/-1)
607 CACGAG
BstYI 188 GGATCC [GA]^GATC[CT]
893 GGATCC
CelII 255 GCTGAGC GC^T[ACGT]AGC
696 GCTCAGC
CfoI 825 GCGC GCG^C
858 GCGC
DpnII 189 GATC ^GATC
894 GATC
EagI 56 CGGCCG C^GGCCG
679 CGGCCG
EclXI 56 CGGCCG C^GGCCG
679 CGGCCG
Eco52I 56 CGGCCG C^GGCCG
679 CGGCCG
EcoT14I 302 CCAAGG C^C[AT][AT]GG
356 CCAAGG
HhaI 825 GCGC GCG^C
858 GCGC
HinP1I 825 GCGC G^CGC
858 GCGC
MboI 189 GATC ^GATC
894 GATC
MflI 188 GGATCC [GA]^GATC[CT]
893 GGATCC
NdeII 189 GATC ^GATC
894 GATC
PvuII 70 CAGCTG CAG^CTG
349 CAGCTG
Sau3AI 189 GATC ^GATC
894 GATC
SfaNI 372 GATGC GCATC(5/9)
603 GCATC
SfcI 643 CTGCAG C^T[GA][CT]AG
996 CTACAG
StyI 302 CCAAGG C^C[AT][AT]GG
356 CCAAGG
TfiI 116 GATTC G^A[AT]TC
124 GATTC
XhoII 188 GGATCC [GA]^GATC[CT]
893 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
BbvI BcnI BpmI BsaJI BsiEI
BsiYI BslI BsmAI BsmFI BspMI
BsrI BssKI BstF5I BstNI BstXI
Cac8I Cfr13I DdeI EaeI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeII
MaeIII MnlI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV PflMI
RsaI Sau96I ScrFI TseI Tsp45I
TspRI Van91I