Restriction map for
pFN21AE2410
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BamHI BbeI BbrPI
BbsI BcgI BclI BcnI BfrI
BglI BglII BlnI BpmI BpuAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
Bst1107I BstBI BstEII BstF5I BstPI
BstUI BstYI BstZ17I Bsu36I ClaI
CpoI DraI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FokI FseI HaeII
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MflI
MluI MroI MspA1I MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp509I Tth111I Van91I XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AciI 489 CCGC CCGC(-3/-1)
AlwI 717 GGATC GGATC(4/5)
AspHI 194 GAGCTC G[AT]GC[AT]^C
AviII 473 TGCGCA TGC^GCA
BbvCI 175 CCTCAGC CCTCAGC(-5/-2)
BciVI 276 GTATCC GTATCC(6/5)
BlpI 70 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 70 GCTCAGC GC^T[ACGT]AGC
BsaAI 188 TACGTA [CT]AC^GT[GA]
BsiHKAI 194 GAGCTC G[AT]GC[AT]^C
BsmFI 724 GGGAC GGGAC(10/14)
BspMI 180 GCAGGT ACCTGC(4/8)
BsrFI 355 ACCGGC [GA]^CCGG[CT]
BssSI 206 CACGAG CACGAG(-5/-1)
BstXI 105 CCAGAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 70 GCTCAGC GC^T[ACGT]AGC
CfoI 474 GCGC GCG^C
Cfr10I 355 ACCGGC [GA]^CCGG[CT]
DpnII 718 GATC ^GATC
DraII 724 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 463 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 724 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 713 CCTTGG C^C[AT][AT]GG
FspI 473 TGCGCA TGC^GCA
HapII 356 CCGG C^CGG
HhaI 474 GCGC GCG^C
HinP1I 474 GCGC G^CGC
HincII 289 GTTGAC GT[CT]^[GA]AC
HindII 289 GTTGAC GT[CT]^[GA]AC
HinfI 434 GAATC G^A[ACGT]TC
HpaII 356 CCGG C^CGG
HphI 247 TCACC GGTGA(8/7)
MaeIII 246 GTCAC ^GT[ACGT]AC
MboI 718 GATC ^GATC
MspI 356 CCGG C^CGG
NdeI 689 CATATG CA^TATG
NdeII 718 GATC ^GATC
PaeR7I 842 CTCGAG C^TCGAG
PpuMI 724 GGGACCT [GA]G^G[AT]CC[CT]
SacI 194 GAGCTC GAGCT^C
Sau3AI 718 GATC ^GATC
SmlI 842 CTCGAG C^T[CT][GA]AG
SnaBI 188 TACGTA TAC^GTA
StyI 713 CCTTGG C^C[AT][AT]GG
TfiI 434 GAATC G^A[AT]TC
Tsp45I 246 GTCAC ^GT[GC]AC
XbaI 793 TCTAGA T^CTAGA
XhoI 842 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AfaI 187 GTAC GT^AC
684 GTAC
AvaI 23 CCCGAG C^[CT]CG[GA]G
842 CTCGAG
AvaII 353 GGACC G^G[AT]CC
725 GGACC
BalI 114 TGGCCA TGG^CCA
507 TGGCCA
BanII 20 GAGCCC G[GA]GC[CT]^C
194 GAGCTC
BsoBI 23 CCCGAG C^[CT]CG[GA]G
842 CTCGAG
BsrDI 66 CATTGC GCAATG(2/0)
545 GCAATG
BstAPI 462 GCACCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
768 GCATCGAGTGC
Cac8I 18 GCGAGC GC[ACGT]^[ACGT]GC
75 GCCAGC
Cfr13I 353 GGACC G^G[ACGT]CC
725 GGACC
EaeI 114 TGGCCA [CT]^GGCC[GA]
507 TGGCCA
EarI 496 GAAGAG CTCTTC(1/4)
670 GAAGAG
Ksp632I 496 GAAGAG CTCTTC(1/4)
670 GAAGAG
MaeII 189 ACGT A^CGT
293 ACGT
MluNI 114 TGGCCA TGG^CCA
507 TGGCCA
MscI 114 TGGCCA TGG^CCA
507 TGGCCA
MseI 53 TTAA T^TAA
733 TTAA
MslI 571 CACAGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
687 CACATATGTG
RsaI 187 GTAC GT^AC
684 GTAC
Sau96I 353 GGACC G^G[ACGT]CC
725 GGACC
TaqI 771 TCGA T^CGA
843 TCGA
Tru9I 53 TTAA T^TAA
733 TTAA
TthHB8I 771 TCGA T^CGA
843 TCGA
XcmI 102 CCACCAGAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
448 CCAAATCCTCACTGG
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AluI BanI BbvI BfaI BmyI
BsaJI BsiYI BslI Bsp1286I BsrI
BssKI BstNI DdeI EcoRII Fnu4HI
HaeIII ItaI MaeI MboII MnlI
MvaI MwoI NlaIII NlaIV ScrFI
SfaNI TseI TspRI