Restriction map for
pFN21AB7609
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BbeI BbvCI BcgI BclI BfaI
BfrI BglI BlnI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HhaI
HinP1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MluI MluNI MroI MscI
MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tth111I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 640 CGCG CG^CG
AcyI 703 GGCGTC G[GA]^CG[CT]C
AlwNI 325 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 675 ATTAAT AT^TAAT
AsnI 675 ATTAAT AT^TAAT
AspHI 247 GAGCAC G[AT]GC[AT]^C
AvaII 149 GGACC G^G[AT]CC
BanII 503 GGGCTC G[GA]GC[CT]^C
BbrPI 87 CACGTG CAC^GTG
BciVI 215 GTATCC GTATCC(6/5)
BglII 509 AGATCT A^GATCT
BsaAI 87 CACGTG [CT]AC^GT[GA]
BsaHI 703 GGCGTC G[GA]^CG[CT]C
BseRI 307 CTCCTC GAGGAG(10/8)
BsgI 348 GTGCAG GTGCAG(16/14)
BsiHKAI 247 GAGCAC G[AT]GC[AT]^C
BsmI 870 GAATGC GAATGC(1/-1)
BspHI 416 TCATGA T^CATGA
BsrDI 92 GCAATG GCAATG(2/0)
BstUI 640 CGCG CG^CG
BstXI 194 CCAACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 509 AGATCT [GA]^GATC[CT]
Cfr13I 149 GGACC G^G[ACGT]CC
EaeI 342 CGGCCA [CT]^GGCC[GA]
EcoRI 631 GAATTC G^AATTC
FauI 298 GCGGG CCCGC(4/6)
HgaI 704 GCGTC GACGC(5/10)
Hin1I 703 GGCGTC G[GA]^CG[CT]C
MaeII 88 ACGT A^CGT
MfeI 210 CAATTG C^AATTG
MflI 509 AGATCT [GA]^GATC[CT]
MseI 676 TTAA T^TAA
MspA1I 199 CAGCTG C[AC]G^C[GT]G
MunI 210 CAATTG C^AATTG
MvnI 640 CGCG CG^CG
MwoI 289 GCAGGGACGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 173 GGCTCC GG[ACGT]^[ACGT]CC
PmaCI 87 CACGTG CAC^GTG
PmlI 87 CACGTG CAC^GTG
PshBI 675 ATTAAT AT^TAAT
PstI 255 CTGCAG CTGCA^G
PvuII 199 CAGCTG CAG^CTG
RcaI 416 TCATGA T^CATGA
Sau96I 149 GGACC G^G[ACGT]CC
ScaI 143 AGTACT AGT^ACT
SexAI 158 ACCAGGT A^CC[AT]GGT
SmaI 281 CCCGGG CCC^GGG
TaqI 233 TCGA T^CGA
Tru9I 676 TTAA T^TAA
TthHB8I 233 TCGA T^CGA
XhoII 509 AGATCT [GA]^GATC[CT]
XmaCI 281 CCCGGG C^CCGGG
XmaI 281 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 144 GTAC GT^AC
156 GTAC
BlpI 736 GCTCAGC GC^T[ACGT]AGC
786 GCTCAGC
BmyI 247 GAGCAC G[AGT]GC[ACT]^C
503 GGGCTC
BpmI 175 CTCCAG CTGGAG(16/14)
826 CTGGAG
Bpu1102I 736 GCTCAGC GC^T[ACGT]AGC
786 GCTCAGC
BsmFI 292 GGGAC GGGAC(10/14)
616 GGGAC
Bsp1286I 247 GAGCAC G[AGT]GC[ACT]^C
503 GGGCTC
Cac8I 63 GCCAGC GC[ACGT]^[ACGT]GC
245 GCGAGC
CelII 736 GCTCAGC GC^T[ACGT]AGC
786 GCTCAGC
DpnII 510 GATC ^GATC
695 GATC
HaeIII 118 GGCC GG^CC
343 GGCC
MboI 510 GATC ^GATC
695 GATC
NdeII 510 GATC ^GATC
695 GATC
PleI 618 GACTC GAGTC(4/5)
547 GAGTC
RsaI 144 GTAC GT^AC
156 GTAC
Sau3AI 510 GATC ^GATC
695 GATC
SfaNI 205 GATGC GCATC(5/9)
327 GCATC
SfcI 255 CTGCAG C^T[GA][CT]AG
312 CTACAG
TfiI 3 GATTC G^A[AT]TC
27 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaI
BbsI BbvI BcnI BpuAI BsaJI
BsiYI BslI BsoBI BsrI BssKI
BstF5I BstNI DdeI EcoRII Fnu4HI
FokI HapII HinfI HpaII HphI
ItaI MaeIII MboII MnlI MspI
MvaI NciI NlaIII ScrFI TseI
Tsp45I Tsp509I TspRI