Restriction map for
pFN21AE1968
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
Acc65I AccII AccIII AclI AflIII
AhdI AocI Aor51HI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbvCI BcgI BlnI BlpI Bpu1102I
BseAI BsgI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
Eco81I EcoO65I EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KasI KpnI KspI MluI MroI
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PmeI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspBI StuI SwaI
TaqI Tth111I TthHB8I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AatII 1278 GACGTC GACGT^C
AccI 1072 GTCTAC GT^[AC][GT]AC
AcyI 1278 GACGTC G[GA]^CG[CT]C
AflII 292 CTTAAG C^TTAAG
AgeI 420 ACCGGT A^CCGGT
Alw44I 1341 GTGCAC G^TGCAC
ApaI 1413 GGGCCC GGGCC^C
ApaLI 1341 GTGCAC G^TGCAC
Asp700I 940 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 527 TGGCCA TGG^CCA
BanII 1413 GGGCCC G[GA]GC[CT]^C
BbrPI 536 CACGTG CAC^GTG
BbsI 1227 GAAGAC GAAGAC(2/6)
BclI 852 TGATCA T^GATCA
BcnI 33 CCGGG CC^[GC]GG
BfaI 617 CTAG C^TAG
BfrI 292 CTTAAG C^TTAAG
BglI 520 GCCGCATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1227 GAAGAC GAAGAC(2/6)
BsaAI 536 CACGTG [CT]AC^GT[GA]
BsaBI 112 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1278 GACGTC G[GA]^CG[CT]C
BsaI 1585 GGTCTC GGTCTC(1/5)
BsaWI 420 ACCGGT [AT]^CCGG[AT]
BseRI 52 GAGGAG GAGGAG(10/8)
BsiEI 30 CGGCCG CG[GA][CT]^CG
BsmAI 1586 GTCTC GTCTC(1/5)
BsmFI 83 GGGAC GGGAC(10/14)
BstAPI 505 GCACTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 334 CCAGTGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 126 CCACGG C^C[GA][CT]GG
EagI 30 CGGCCG C^GGCCG
EarI 352 GAAGAG CTCTTC(1/4)
EclXI 30 CGGCCG C^GGCCG
Eco52I 30 CGGCCG C^GGCCG
EcoNI 633 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 340 CCTTGG C^C[AT][AT]GG
FauI 80 GCGGG CCCGC(4/6)
FbaI 852 TGATCA T^GATCA
HgaI 13 GCGTC GACGC(5/10)
Hin1I 1278 GACGTC G[GA]^CG[CT]C
Ksp632I 352 GAAGAG CTCTTC(1/4)
MaeI 617 CTAG C^TAG
MamI 112 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1349 CAATTG C^AATTG
MluNI 527 TGGCCA TGG^CCA
MscI 527 TGGCCA TGG^CCA
MspA1I 78 CAGCGG C[AC]G^C[GT]G
MunI 1349 CAATTG C^AATTG
NciI 33 CCGGG CC^[GC]GG
NdeI 490 CATATG CA^TATG
NlaIV 1413 GGGCCC GG[ACGT]^[ACGT]CC
NspI 1125 ACATGC [GA]CATG^[CT]
PflMI 873 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 420 ACCGGT A^CCGGT
PmaCI 536 CACGTG CAC^GTG
PmlI 536 CACGTG CAC^GTG
PpuMI 56 AGGACCT [GA]G^G[AT]CC[CT]
SmlI 292 CTTAAG C^T[CT][GA]AG
SpeI 616 ACTAGT A^CTAGT
SspI 220 AATATT AAT^ATT
StyI 340 CCTTGG C^C[AT][AT]GG
Van91I 873 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 940 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 312 GTAC GT^AC
430 GTAC
AlwNI 597 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
1199 CAGAAGCTG
AvaII 57 GGACC G^G[AT]CC
630 GGACC
BbvI 135 GCAGC GCAGC(8/12)
817 GCTGC
BciVI 262 GGATAC GTATCC(6/5)
683 GGATAC
BglII 117 AGATCT A^GATCT
408 AGATCT
BpmI 634 CTCCAG CTGGAG(16/14)
1588 CTCCAG
BsrDI 624 CATTGC GCAATG(2/0)
877 GCAATG
BsrFI 25 ACCGGC [GA]^CCGG[CT]
420 ACCGGT
Cac8I 131 GCAAGC GC[ACGT]^[ACGT]GC
1354 GCTGGC
CfoI 20 GCGC GCG^C
38 GCGC
Cfr10I 25 ACCGGC [GA]^CCGG[CT]
420 ACCGGT
DraII 56 AGGACCT [GA]G^G[ACGT]CC[CT]
1413 GGGCCCT
EaeI 30 CGGCCG [CT]^GGCC[GA]
527 TGGCCA
EcoO109I 56 AGGACCT [GA]G^G[ACGT]CC[CT]
1413 GGGCCCT
EcoRI 318 GAATTC G^AATTC
800 GAATTC
HaeII 19 GGCGCT [GA]GCGC^[CT]
37 GGCGCT
HhaI 20 GCGC GCG^C
38 GCGC
HinP1I 20 GCGC G^CGC
38 GCGC
HphI 1486 GGTGA GGTGA(8/7)
1544 TCACC
RsaI 312 GTAC GT^AC
430 GTAC
SfcI 302 CTACAG C^T[GA][CT]AG
956 CTGTAG
TfiI 297 GAATC G^A[AT]TC
1024 GATTC
TseI 135 GCAGC G^C[AT]GC
817 GCTGC
Tsp45I 1507 GTCAC ^GT[GC]AC
1549 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AspHI BmyI BsaJI BsiHKAI BsiYI
BslI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII PleI Sau3AI Sau96I
ScrFI SfaNI Tru9I Tsp509I TspRI
XhoII