Restriction map for
pFN21AE2337
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbvCI BcgI BfrI
BglI BlnI BsaBI BsaWI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoT22I
FauI FseI FspI HaeII HpaI
KasI KpnI KspI MamI MluI
MluNI MroI MscI MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcyI 1602 GACGCC G[GA]^CG[CT]C
Alw44I 1632 GTGCAC G^TGCAC
ApaLI 1632 GTGCAC G^TGCAC
AvaI 1828 CTCGAG C^[CT]CG[GA]G
BanI 571 GGCACC G^G[CT][GA]CC
BbrPI 1154 CACGTG CAC^GTG
BbsI 1116 GTCTTC GAAGAC(2/6)
BciVI 1909 GGATAC GTATCC(6/5)
BclI 623 TGATCA T^GATCA
BcnI 2145 CCCGG CC^[GC]GG
BglII 1393 AGATCT A^GATCT
BpuAI 1116 GTCTTC GAAGAC(2/6)
BsaAI 1154 CACGTG [CT]AC^GT[GA]
BsaHI 1602 GACGCC G[GA]^CG[CT]C
BsaI 2103 GGTCTC GGTCTC(1/5)
BseRI 592 GAGGAG GAGGAG(10/8)
BsmBI 1110 GAGACG CGTCTC(1/5)
BsmI 1437 GAATGC GAATGC(1/-1)
BsoBI 1828 CTCGAG C^[CT]CG[GA]G
BspLU11I 725 ACATGT A^CATGT
BsrFI 1036 ACCGGC [GA]^CCGG[CT]
Bst1107I 287 GTATAC GTA^TAC
BstXI 2379 CCAGCCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 287 GTATAC GTA^TAC
CfoI 1240 GCGC GCG^C
Cfr10I 1036 ACCGGC [GA]^CCGG[CT]
DraIII 1926 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2456 TGGCCG [CT]^GGCC[GA]
EcoRI 1284 GAATTC G^AATTC
FbaI 623 TGATCA T^GATCA
HhaI 1240 GCGC GCG^C
Hin1I 1602 GACGCC G[GA]^CG[CT]C
HinP1I 1240 GCGC G^CGC
HindIII 1740 AAGCTT A^AGCTT
MfeI 2018 CAATTG C^AATTG
MslI 1591 CACCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 2018 CAATTG C^AATTG
NciI 2145 CCCGG CC^[GC]GG
NdeI 502 CATATG CA^TATG
PaeR7I 1828 CTCGAG C^TCGAG
PflMI 1473 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1154 CACGTG CAC^GTG
PmlI 1154 CACGTG CAC^GTG
PpuMI 1862 AGGACCT [GA]G^G[AT]CC[CT]
SalI 461 GTCGAC G^TCGAC
SphI 1823 GCATGC GCATG^C
Van91I 1473 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1828 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 287 GTATAC GT^[AC][GT]AC
461 GTCGAC
AflIII 725 ACATGT A^C[GA][CT]GT
1153 ACACGT
BanII 210 GAGCCC G[GA]GC[CT]^C
1620 GAGCCC
BfaI 490 CTAG C^TAG
859 CTAG
BlpI 1312 GCTCAGC GC^T[ACGT]AGC
2189 GCTCAGC
Bpu1102I 1312 GCTCAGC GC^T[ACGT]AGC
2189 GCTCAGC
BsgI 1289 CTGCAC GTGCAG(16/14)
2242 GTGCAG
BspMI 1571 ACCTGC ACCTGC(4/8)
2023 GCAGGT
CelII 1312 GCTCAGC GC^T[ACGT]AGC
2189 GCTCAGC
DraI 922 TTTAAA TTT^AAA
1855 TTTAAA
DraII 1541 AGGCCCC [GA]G^G[ACGT]CC[CT]
1862 AGGACCT
DrdI 1455 GACAGCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2356 GACTCACCAGTC
DsaI 166 CCGTGG C^C[GA][CT]GG
2117 CCGTGG
EcoNI 1533 CCTACCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1724 CCTCTACCAGG
EcoO109I 1541 AGGCCCC [GA]G^G[ACGT]CC[CT]
1862 AGGACCT
EcoRV 383 GATATC GAT^ATC
475 GATATC
HapII 1037 CCGG C^CGG
2146 CCGG
HgaI 464 GACGC GACGC(5/10)
1602 GACGC
HincII 461 GTCGAC GT[CT]^[GA]AC
837 GTCAAC
HindII 461 GTCGAC GT[CT]^[GA]AC
837 GTCAAC
HpaII 1037 CCGG C^CGG
2146 CCGG
MaeI 490 CTAG C^TAG
859 CTAG
MspA1I 1384 CAGCTG C[AC]G^C[GT]G
1793 CAGCTG
MspI 1037 CCGG C^CGG
2146 CCGG
MwoI 1312 GCTCAGCTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
2504 GCTCATCCTGC
NspI 725 ACATGT [GA]CATG^[CT]
1823 GCATGC
PstI 1573 CTGCAG CTGCA^G
2212 CTGCAG
PvuII 1384 CAGCTG CAG^CTG
1793 CAGCTG
SapI 579 GAAGAGC GCTCTTC(1/4)
1345 GAAGAGC
SfcI 1573 CTGCAG C^T[GA][CT]AG
2212 CTGCAG
StuI 1614 AGGCCT AGG^CCT
1732 AGGCCT
XcmI 105 CCAGCCTGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1021 CCAGCTTTCAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaII
BbvI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I Cfr13I DdeI DpnII
EarI Eco81I EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MseI MvaI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII