Restriction map for
pFN21AE9518
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
ApaLI ApoI AscI AseI AsnI
AspEI AspI AvaI AvrII BalI
BbeI BcgI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DsaI Eam1105I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
Hin1I HincII HindII HpaI HphI
KasI KspI MamI MfeI MluNI
MroI MscI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI TaqI
Tsp509I Tth111I TthHB8I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 63 enzymes) #
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Acc65I 386 GGTACC G^GTACC
AflIII 890 ACGCGT A^C[GA][CT]GT
AlwNI 853 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 925 CCTGAGG CC^T[ACGT]AGG
Aor51HI 356 AGCGCT AGC^GCT
ApaI 440 GGGCCC GGGCC^C
Asp700I 174 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 386 GGTACC G^GTACC
AviII 167 TGCGCA TGC^GCA
BanI 386 GGTACC G^G[CT][GA]CC
BbrPI 727 CACGTG CAC^GTG
BbsI 670 GTCTTC GAAGAC(2/6)
BbvCI 225 CCTCAGC CCTCAGC(-5/-2)
BciVI 266 GTATCC GTATCC(6/5)
BclI 596 TGATCA T^GATCA
BcnI 617 CCCGG CC^[GC]GG
BfaI 763 CTAG C^TAG
BpuAI 670 GTCTTC GAAGAC(2/6)
BsiEI 231 CGGCCG CG[GA][CT]^CG
BsmAI 58 GAGAC GTCTC(1/5)
BsmFI 447 GTCCC GGGAC(10/14)
BsmI 698 GCATTC GAATGC(1/-1)
BsrBI 782 CCGCTC CCGCTC(-3/-3)
BsrI 666 ACTGG ACTGG(1/-1)
Bsu36I 925 CCTGAGG CC^T[ACGT]AGG
DraII 440 GGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 733 GACAGGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 231 CGGCCG C^GGCCG
EarI 220 CTCTTC CTCTTC(1/4)
EclXI 231 CGGCCG C^GGCCG
Eco47III 356 AGCGCT AGC^GCT
Eco52I 231 CGGCCG C^GGCCG
Eco81I 925 CCTGAGG CC^T[ACGT]AGG
EcoO109I 440 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 20 CCTTGG C^C[AT][AT]GG
FbaI 596 TGATCA T^GATCA
FspI 167 TGCGCA TGC^GCA
HaeII 356 AGCGCT [GA]GCGC^[CT]
HapII 618 CCGG C^CGG
HindIII 255 AAGCTT A^AGCTT
HpaII 618 CCGG C^CGG
KpnI 386 GGTACC GGTAC^C
Ksp632I 220 CTCTTC CTCTTC(1/4)
MaeI 763 CTAG C^TAG
MaeIII 646 GTGAC ^GT[ACGT]AC
MluI 890 ACGCGT A^CGCGT
MspI 618 CCGG C^CGG
NciI 617 CCCGG CC^[GC]GG
NotI 230 GCGGCCGC GC^GGCCGC
NspI 164 GCATGC [GA]CATG^[CT]
PleI 60 GACTC GAGTC(4/5)
PmaCI 727 CACGTG CAC^GTG
PmlI 727 CACGTG CAC^GTG
PstI 883 CTGCAG CTGCA^G
ScaI 663 AGTACT AGT^ACT
SfcI 883 CTGCAG C^T[GA][CT]AG
SmlI 81 CTTGAG C^T[CT][GA]AG
SphI 164 GCATGC GCATG^C
StuI 324 AGGCCT AGG^CCT
StyI 20 CCTTGG C^C[AT][AT]GG
Tsp45I 646 GTGAC ^GT[GC]AC
XcmI 11 CCATCTCAACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 174 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
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AspHI 142 GTGCTC G[AT]GC[AT]^C
348 GAGCAC
AvaII 578 GGTCC G^G[AT]CC
588 GGTCC
BsaAI 643 TACGTG [CT]AC^GT[GA]
727 CACGTG
BsaJI 20 CCTTGG C^C[ACGT][ACGT]GG
153 CCTGGG
BseRI 676 CTCCTC GAGGAG(10/8)
55 GAGGAG
BsiHKAI 142 GTGCTC G[AT]GC[AT]^C
348 GAGCAC
CfoI 168 GCGC GCG^C
357 GCGC
EaeI 203 TGGCCG [CT]^GGCC[GA]
231 CGGCCG
HhaI 168 GCGC GCG^C
357 GCGC
HinP1I 168 GCGC G^CGC
357 GCGC
MseI 253 TTAA T^TAA
788 TTAA
NlaIII 165 CATG CATG^
754 CATG
SfaNI 104 GCATC GCATC(5/9)
461 GCATC
TfiI 505 GATTC G^A[AT]TC
603 GATTC
Tru9I 253 TTAA T^TAA
788 TTAA
TspRI 495 CAGTG CA[GC]TG[ACGT][ACGT]^
795 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
BamHI BanII BbvI BmyI BpmI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI BstUI BstYI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HgaI HinfI ItaI MaeII
MboI MboII MflI MnlI MspA1I
MvaI MvnI MwoI NdeII NlaIV
RsaI Sau3AI Sau96I ScrFI TseI
XhoII