Restriction map for
pFN21AE9509
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AvaI
AvrII BalI BbeI BbrPI BbvCI
BcgI BclI BfrI BglI BglII
BlnI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
Eam1105I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
Hin1I HincII HindII HindIII HpaI
HphI KasI KspI MamI MfeI
MluI MluNI MroI MscI MslI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI StyI SwaI
TaqI Tsp509I Tth111I TthHB8I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 386 GGTACC G^GTACC
Aor51HI 356 AGCGCT AGC^GCT
Asp718I 386 GGTACC G^GTACC
AspHI 142 GTGCTC G[AT]GC[AT]^C
AviII 167 TGCGCA TGC^GCA
BanI 386 GGTACC G^G[CT][GA]CC
BbsI 670 GTCTTC GAAGAC(2/6)
BciVI 461 GTATCC GTATCC(6/5)
BcnI 617 CCCGG CC^[GC]GG
BfaI 763 CTAG C^TAG
BlpI 336 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 336 GCTGAGC GC^T[ACGT]AGC
BpuAI 670 GTCTTC GAAGAC(2/6)
BsaAI 643 TACGTG [CT]AC^GT[GA]
BsiEI 209 CGGCCG CG[GA][CT]^CG
BsiHKAI 142 GTGCTC G[AT]GC[AT]^C
BsmFI 447 GTCCC GGGAC(10/14)
BsmI 698 GCATTC GAATGC(1/-1)
BsrBI 782 CCGCTC CCGCTC(-3/-3)
BsrI 16 CCAGT ACTGG(1/-1)
CelII 336 GCTGAGC GC^T[ACGT]AGC
EagI 209 CGGCCG C^GGCCG
EarI 220 CTCTTC CTCTTC(1/4)
EclXI 209 CGGCCG C^GGCCG
Eco47III 356 AGCGCT AGC^GCT
Eco52I 209 CGGCCG C^GGCCG
FspI 167 TGCGCA TGC^GCA
HapII 618 CCGG C^CGG
HgaI 840 GCGTC GACGC(5/10)
HpaII 618 CCGG C^CGG
KpnI 386 GGTACC GGTAC^C
Ksp632I 220 CTCTTC CTCTTC(1/4)
MaeI 763 CTAG C^TAG
MseI 788 TTAA T^TAA
MspA1I 628 CCGCTG C[AC]G^C[GT]G
MspI 618 CCGG C^CGG
NciI 617 CCCGG CC^[GC]GG
PflMI 368 CCATCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 60 GACTC GAGTC(4/5)
PstI 883 CTGCAG CTGCA^G
SexAI 200 ACCTGGT A^CC[AT]GGT
SfcI 883 CTGCAG C^T[GA][CT]AG
StuI 324 AGGCCT AGG^CCT
Tru9I 788 TTAA T^TAA
Van91I 368 CCATCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 76 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
853 CAGAACCTG
AocI 895 CCTGAGG CC^T[ACGT]AGG
925 CCTCAGG
ApaI 440 GGGCCC GGGCC^C
707 GGGCCC
AvaII 578 GGTCC G^G[AT]CC
588 GGTCC
BamHI 613 GGATCC G^GATCC
722 GGATCC
BsaJI 153 CCTGGG C^C[ACGT][ACGT]GG
935 CCCTGG
BsmAI 58 GAGAC GTCTC(1/5)
181 GTCTC
BstYI 613 GGATCC [GA]^GATC[CT]
722 GGATCC
Bsu36I 895 CCTGAGG CC^T[ACGT]AGG
925 CCTCAGG
DpnII 614 GATC ^GATC
723 GATC
DraII 440 GGGCCCT [GA]G^G[ACGT]CC[CT]
707 GGGCCCT
EaeI 209 CGGCCG [CT]^GGCC[GA]
231 CGGCCA
Eco81I 895 CCTGAGG CC^T[ACGT]AGG
925 CCTCAGG
EcoO109I 440 GGGCCCT [GA]G^G[ACGT]CC[CT]
707 GGGCCCT
HaeII 340 AGCGCC [GA]GCGC^[CT]
356 AGCGCT
MboI 614 GATC ^GATC
723 GATC
MflI 613 GGATCC [GA]^GATC[CT]
722 GGATCC
NdeII 614 GATC ^GATC
723 GATC
NspI 164 GCATGC [GA]CATG^[CT]
332 GCATGC
Sau3AI 614 GATC ^GATC
723 GATC
SphI 164 GCATGC GCATG^C
332 GCATGC
Tsp45I 482 GTGAC ^GT[GC]AC
646 GTGAC
XcmI 11 CCATCCCAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
719 CCAGGATCCACCTGG
XhoII 613 GGATCC [GA]^GATC[CT]
722 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
BanII BbvI BmyI BpmI BseRI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI EcoRII Fnu4HI FokI HaeIII
HhaI HinP1I HinfI ItaI MaeII
MaeIII MboII MnlI MvaI MvnI
MwoI NlaIII NlaIV RsaI Sau96I
ScrFI SfaNI TfiI TseI TspRI