Restriction map for
pFN21AE4091
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BanI
BbeI BbrPI BbsI BbvCI BciVI
BclI BcnI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII Eam1105I Eco47III Eco81I
EcoNI EcoT22I FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI MamI MfeI
MluI MroI MslI MunI NaeI
NarI NciI NdeI NgoMIV NheI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tth111I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 630 GAATTC [GA]^AATT[CT]
ApaI 1118 GGGCCC GGGCC^C
ApoI 630 GAATTC [GA]^AATT[CT]
Asp700I 126 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 445 GAGCTC G[AT]GC[AT]^C
BamHI 376 GGATCC G^GATCC
BsgI 1132 GTGCAG GTGCAG(16/14)
BsiEI 41 CGGCCG CG[GA][CT]^CG
BsiHKAI 445 GAGCTC G[AT]GC[AT]^C
BsrFI 1128 GCCGGT [GA]^CCGG[CT]
BstEII 1239 GGTCACC G^GT[ACGT]ACC
BstPI 1239 GGTCACC G^GT[ACGT]ACC
BstYI 376 GGATCC [GA]^GATC[CT]
Cfr10I 1128 GCCGGT [GA]^CCGG[CT]
DrdI 1231 GACCCCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 41 CGGCCG C^GGCCG
EclXI 41 CGGCCG C^GGCCG
Eco52I 41 CGGCCG C^GGCCG
EcoO65I 1239 GGTCACC G^GT[ACGT]ACC
EcoRI 630 GAATTC G^AATTC
EcoRV 262 GATATC GAT^ATC
FauI 34 CCCGC CCCGC(4/6)
HaeII 16 AGCGCC [GA]GCGC^[CT]
HapII 1129 CCGG C^CGG
HpaII 1129 CCGG C^CGG
KspI 35 CCGCGG CCGC^GG
MaeII 1112 ACGT A^CGT
MflI 376 GGATCC [GA]^GATC[CT]
MspI 1129 CCGG C^CGG
NcoI 1289 CCATGG C^CATGG
NotI 40 GCGGCCGC GC^GGCCGC
PpuMI 1229 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 877 GACTATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 182 CTGCAG CTGCA^G
PvuII 1201 CAGCTG CAG^CTG
SacI 445 GAGCTC GAGCT^C
SacII 35 CCGCGG CCGC^GG
SapI 48 GCTCTTC GCTCTTC(1/4)
SexAI 827 ACCTGGT A^CC[AT]GGT
XbaI 624 TCTAGA T^CTAGA
XhoII 376 GGATCC [GA]^GATC[CT]
XmnI 126 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
BalI 390 TGGCCA TGG^CCA
1179 TGGCCA
BcgI 556 CGACGGTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
965 CGAACAGCATGC
BglI 19 GCCTCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
31 GCCCCCGCGGC
BpmI 810 CTGGAG CTGGAG(16/14)
998 CTGGAG
BsaI 203 GAGACC GGTCTC(1/5)
1011 GGTCTC
BseRI 167 GAGGAG GAGGAG(10/8)
200 GAGGAG
BsrDI 988 CATTGC GCAATG(2/0)
640 GCAATG
BstAPI 493 GCAGTAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1134 GCAGGCTCTGC
BstF5I 306 CATCC GGATG(2/0)
817 GGATG
DpnII 159 GATC ^GATC
377 GATC
DraII 1118 GGGCCCC [GA]G^G[ACGT]CC[CT]
1229 AGGACCC
DsaI 35 CCGCGG C^C[GA][CT]GG
1289 CCATGG
EarI 49 CTCTTC CTCTTC(1/4)
578 CTCTTC
EcoO109I 1118 GGGCCCC [GA]G^G[ACGT]CC[CT]
1229 AGGACCC
FokI 306 CATCC GGATG(9/13)
817 GGATG
HphI 1212 TCACC GGTGA(8/7)
1241 TCACC
Ksp632I 49 CTCTTC CTCTTC(1/4)
578 CTCTTC
MboI 159 GATC ^GATC
377 GATC
MluNI 390 TGGCCA TGG^CCA
1179 TGGCCA
MscI 390 TGGCCA TGG^CCA
1179 TGGCCA
MseI 679 TTAA T^TAA
994 TTAA
MspA1I 35 CCGCGG C[AC]G^C[GT]G
1201 CAGCTG
NdeII 159 GATC ^GATC
377 GATC
PleI 576 GACTC GAGTC(4/5)
1157 GACTC
Sau3AI 159 GATC ^GATC
377 GATC
SfcI 182 CTGCAG C^T[GA][CT]AG
503 CTACAG
SphI 489 GCATGC GCATG^C
971 GCATGC
TaqI 417 TCGA T^CGA
1265 TCGA
TfiI 937 GATTC G^A[AT]TC
1276 GAATC
Tru9I 679 TTAA T^TAA
994 TTAA
TthHB8I 417 TCGA T^CGA
1265 TCGA
XcmI 305 CCATCCAACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
525 CCAGTGGGGGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AvaII BanII BbvI BfaI BmyI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstNI BstUI
Cac8I CfoI Cfr13I DdeI EaeI
EcoRII EcoT14I Fnu4HI HaeIII HhaI
HinP1I HinfI ItaI MaeI MaeIII
MboII MnlI MvaI MvnI MwoI
NlaIII NlaIV NspI PflMI Sau96I
ScrFI SfaNI StyI TseI Tsp45I
Tsp509I TspRI Van91I