Restriction map for
pFN21AE6740
##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BclI BfaI BfrI BglII
BlnI BsaAI BsaBI BsiWI BsmBI
Bsp1407I BspDI BsrGI BssHII BssSI
Bst1107I BstZ17I ClaI CpoI DraI
DrdI Eam1105I Eco47III EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
HincII HindII HindIII HpaI KasI
KpnI MaeI MamI MfeI MluI
MunI NarI NcoI NdeI NheI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SalI SapI SbfI SfiI SfoI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 1660 GACGTC GACGT^C
AccI 1411 GTCTAC GT^[AC][GT]AC
AcsI 1975 GAATTC [GA]^AATT[CT]
AcyI 1660 GACGTC G[GA]^CG[CT]C
AocI 1366 CCTGAGG CC^T[ACGT]AGG
ApaI 318 GGGCCC GGGCC^C
ApoI 1975 GAATTC [GA]^AATT[CT]
AvaI 1301 CCCGGG C^[CT]CG[GA]G
BalI 491 TGGCCA TGG^CCA
BanI 1443 GGTGCC G^G[CT][GA]CC
BbsI 643 GAAGAC GAAGAC(2/6)
BciVI 585 GGATAC GTATCC(6/5)
BglI 331 GCCAGTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 643 GAAGAC GAAGAC(2/6)
BsaHI 1660 GACGTC G[GA]^CG[CT]C
BsmI 2245 GCATTC GAATGC(1/-1)
BsoBI 1301 CCCGGG C^[CT]CG[GA]G
BspHI 888 TCATGA T^CATGA
BsrBI 2136 GAGCGG CCGCTC(-3/-3)
BstBI 2213 TTCGAA TT^CGAA
BstEII 423 GGTCACC G^GT[ACGT]ACC
BstPI 423 GGTCACC G^GT[ACGT]ACC
Bsu36I 1366 CCTGAGG CC^T[ACGT]AGG
DraIII 1810 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1366 CCTGAGG CC^T[ACGT]AGG
EcoO65I 423 GGTCACC G^GT[ACGT]ACC
EcoRI 1975 GAATTC G^AATTC
Hin1I 1660 GACGTC G[GA]^CG[CT]C
MluNI 491 TGGCCA TGG^CCA
MscI 491 TGGCCA TGG^CCA
NotI 985 GCGGCCGC GC^GGCCGC
NspV 2213 TTCGAA TT^CGAA
PpuMI 2193 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 888 TCATGA T^CATGA
SacI 2272 GAGCTC GAGCT^C
SexAI 1811 ACCTGGT A^CC[AT]GGT
SfuI 2213 TTCGAA TT^CGAA
SgrAI 850 CACCGGCG C[GA]^CCGG[CT]G
SmaI 1301 CCCGGG CCC^GGG
SspI 1952 AATATT AAT^ATT
XmaCI 1301 CCCGGG C^CCGGG
XmaI 1301 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 597 TCCGGA T^CCGGA
2237 TCCGGA
AflIII 1631 ACATGT A^C[GA][CT]GT
1709 ACATGT
AlwNI 277 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
562 CAGTACCTG
Asp700I 252 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1509 GAACATCTTC
BlpI 2088 GCTGAGC GC^T[ACGT]AGC
2274 GCTCAGC
Bpu1102I 2088 GCTGAGC GC^T[ACGT]AGC
2274 GCTCAGC
BseAI 597 TCCGGA T^CCGGA
2237 TCCGGA
BsgI 590 CTGCAC GTGCAG(16/14)
1111 CTGCAC
BspEI 597 TCCGGA T^CCGGA
2237 TCCGGA
BspLU11I 1631 ACATGT A^CATGT
1709 ACATGT
BspMI 2095 ACCTGC ACCTGC(4/8)
1095 GCAGGT
BstAPI 394 GCACCCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1008 GCATCTGCTGC
BstXI 482 CCAAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1718 CCAACATCATGG
BstYI 1904 AGATCC [GA]^GATC[CT]
2234 AGATCC
CelII 2088 GCTGAGC GC^T[ACGT]AGC
2274 GCTCAGC
EcoT14I 188 CCAAGG C^C[AT][AT]GG
494 CCAAGG
MflI 1904 AGATCC [GA]^GATC[CT]
2234 AGATCC
MroI 597 TCCGGA T^CCGGA
2237 TCCGGA
MseI 898 TTAA T^TAA
1577 TTAA
NaeI 1163 GCCGGC GCC^GGC
1795 GCCGGC
NgoMIV 1163 GCCGGC G^CCGGC
1795 GCCGGC
PstI 202 CTGCAG CTGCA^G
2131 CTGCAG
ScaI 1271 AGTACT AGT^ACT
1328 AGTACT
SmlI 1861 CTCAAG C^T[CT][GA]AG
2075 CTCAAG
StyI 188 CCAAGG C^C[AT][AT]GG
494 CCAAGG
TfiI 267 GATTC G^A[AT]TC
871 GAATC
Tru9I 898 TTAA T^TAA
1577 TTAA
Tsp509I 1976 AATT ^AATT
2179 AATT
XhoII 1904 AGATCC [GA]^GATC[CT]
2234 AGATCC
XmnI 252 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1509 GAACATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BanII BbvI BcnI
BmyI BpmI BsaI BsaJI BsaWI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoNI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XcmI