Restriction map for
pFN21AE2478
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI Alw44I AlwNI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AviII AvrII BamHI BbeI
BbrPI BbvCI BglI BlnI BsaBI
BsaWI BseAI BsiEI BsiWI Bsp1407I
BspEI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Cfr10I CpoI DrdI EagI
Eam1105I EclXI Eco52I EcoNI EcoO65I
EcoRI EcoRV FseI FspI HindIII
HpaI KasI KpnI MamI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SpeI SphI Sse8387I
SspBI XbaI XhoI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AcsI 2248 GAATTT [GA]^AATT[CT]
AcyI 175 GACGCC G[GA]^CG[CT]C
AflII 2758 CTTAAG C^TTAAG
AlwI 540 GGATC GGATC(4/5)
AocI 1735 CCTAAGG CC^T[ACGT]AGG
Aor51HI 2644 AGCGCT AGC^GCT
ApoI 2248 GAATTT [GA]^AATT[CT]
Asp700I 2811 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 769 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 617 TGGCCA TGG^CCA
BanI 668 GGTGCC G^G[CT][GA]CC
BciVI 894 GGATAC GTATCC(6/5)
BclI 1002 TGATCA T^GATCA
BfrI 2758 CTTAAG C^TTAAG
BglII 2474 AGATCT A^GATCT
BlpI 1600 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1600 GCTGAGC GC^T[ACGT]AGC
BsaHI 175 GACGCC G[GA]^CG[CT]C
BsaI 2901 GAGACC GGTCTC(1/5)
BsmBI 1400 GAGACG CGTCTC(1/5)
BspDI 259 ATCGAT AT^CGAT
BspHI 360 TCATGA T^CATGA
BspLU11I 958 ACATGT A^CATGT
BsrBI 507 CCGCTC CCGCTC(-3/-3)
BsrDI 989 GCAATG GCAATG(2/0)
BstYI 2474 AGATCT [GA]^GATC[CT]
Bsu36I 1735 CCTAAGG CC^T[ACGT]AGG
CelII 1600 GCTGAGC GC^T[ACGT]AGC
ClaI 259 ATCGAT AT^CGAT
DraI 1057 TTTAAA TTT^AAA
Eco47III 2644 AGCGCT AGC^GCT
Eco81I 1735 CCTAAGG CC^T[ACGT]AGG
EcoT22I 2732 ATGCAT ATGCA^T
FbaI 1002 TGATCA T^GATCA
Hin1I 175 GACGCC G[GA]^CG[CT]C
KspI 116 CCGCGG CCGC^GG
MflI 2474 AGATCT [GA]^GATC[CT]
MluNI 617 TGGCCA TGG^CCA
MscI 617 TGGCCA TGG^CCA
NcoI 654 CCATGG C^CATGG
NsiI 2732 ATGCAT ATGCA^T
PflFI 769 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1086 CCAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 903 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 360 TCATGA T^CATGA
SacI 277 GAGCTC GAGCT^C
SacII 116 CCGCGG CCGC^GG
SapI 1478 GCTCTTC GCTCTTC(1/4)
SexAI 2013 ACCTGGT A^CC[AT]GGT
SmaI 25 CCCGGG CCC^GGG
SnaBI 1672 TACGTA TAC^GTA
SwaI 1056 ATTTAAAT ATTT^AAAT
Tth111I 769 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1086 CCAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 2474 AGATCT [GA]^GATC[CT]
XmaCI 25 CCCGGG C^CCGGG
XmaI 25 CCCGGG C^CCGGG
XmnI 2811 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 9 CGCG CG^CG
117 CGCG
AflIII 853 ACGTGT A^C[GA][CT]GT
958 ACATGT
BbsI 954 GAAGAC GAAGAC(2/6)
2704 GAAGAC
BfaI 157 CTAG C^TAG
2648 CTAG
BpuAI 954 GAAGAC GAAGAC(2/6)
2704 GAAGAC
BstUI 9 CGCG CG^CG
117 CGCG
BstXI 800 CCACATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
942 CCAGTTGGATGG
CfoI 2448 GCGC GCG^C
2645 GCGC
DraIII 1361 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
2412 CACCGAGTG
DsaI 116 CCGCGG C^C[GA][CT]GG
654 CCATGG
EaeI 617 TGGCCA [CT]^GGCC[GA]
1719 TGGCCG
HaeII 2447 AGCGCC [GA]GCGC^[CT]
2644 AGCGCT
HapII 26 CCGG C^CGG
100 CCGG
HgaI 175 GACGC GACGC(5/10)
584 GCGTC
HhaI 2448 GCGC GCG^C
2645 GCGC
HinP1I 2448 GCGC G^CGC
2645 GCGC
HpaII 26 CCGG C^CGG
100 CCGG
MaeI 157 CTAG C^TAG
2648 CTAG
MspI 26 CCGG C^CGG
100 CCGG
MvnI 9 CGCG CG^CG
117 CGCG
NdeI 2552 CATATG CA^TATG
2675 CATATG
NspI 958 ACATGT [GA]CATG^[CT]
1684 ACATGC
PpuMI 1114 AGGACCT [GA]G^G[AT]CC[CT]
2660 AGGTCCC
PstI 915 CTGCAG CTGCA^G
2027 CTGCAG
PvuII 711 CAGCTG CAG^CTG
1128 CAGCTG
SmlI 757 CTTGAG C^T[CT][GA]AG
2758 CTTAAG
SspI 310 AATATT AAT^ATT
2021 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaI
AvaII BanII BbvI BcgI BcnI
BmyI BpmI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HincII HindII HinfI
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI