Restriction map for
pFN21AE5432
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BbeI BbrPI BbsI
BbvCI BcgI BciVI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
BspDI BspEI BspLU11I BsrFI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstZ17I Cac8I CelII Cfr10I
ClaI CpoI DraI DraII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoT22I FauI
FseI FspI HaeII HapII Hin1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MaeI MaeII
MamI MfeI MluI MluNI MroI
MscI MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AfaI 819 GTAC GT^AC
AocI 861 CCTCAGG CC^T[ACGT]AGG
Asp700I 137 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 598 GGATCC G^GATCC
BanI 686 GGCACC G^G[CT][GA]CC
BanII 884 GGGCTC G[GA]GC[CT]^C
BbvI 334 GCTGC GCAGC(8/12)
BclI 95 TGATCA T^GATCA
BsgI 708 CTGCAC GTGCAG(16/14)
BsmFI 745 GGGAC GGGAC(10/14)
BsmI 319 GAATGC GAATGC(1/-1)
Bsp1407I 818 TGTACA T^GTACA
BspMI 706 ACCTGC ACCTGC(4/8)
BsrBI 29 CCGCTC CCGCTC(-3/-3)
BsrDI 450 CATTGC GCAATG(2/0)
BsrGI 818 TGTACA T^GTACA
BssKI 243 CCTGG ^CC[ACGT]GG
BstEII 729 GGTTACC G^GT[ACGT]ACC
BstNI 243 CCTGG CC^[AT]GG
BstPI 729 GGTTACC G^GT[ACGT]ACC
BstXI 602 CCATCATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 598 GGATCC [GA]^GATC[CT]
Bsu36I 861 CCTCAGG CC^T[ACGT]AGG
CfoI 672 GCGC GCG^C
DraIII 615 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 338 CCATGG C^C[GA][CT]GG
Eco81I 861 CCTCAGG CC^T[ACGT]AGG
EcoO65I 729 GGTTACC G^GT[ACGT]ACC
EcoRI 3 GAATTC G^AATTC
EcoRII 243 CCTGG ^CC[AT]GG
EcoRV 559 GATATC GAT^ATC
FbaI 95 TGATCA T^GATCA
HgaI 323 GCGTC GACGC(5/10)
HhaI 672 GCGC GCG^C
HinP1I 672 GCGC G^CGC
MflI 598 GGATCC [GA]^GATC[CT]
MspA1I 296 CAGCGG C[AC]G^C[GT]G
MvaI 243 CCTGG CC^[AT]GG
NcoI 338 CCATGG C^CATGG
NspI 227 ACATGC [GA]CATG^[CT]
PleI 80 GAGTC GAGTC(4/5)
RsaI 819 GTAC GT^AC
ScrFI 243 CCTGG CC^[ACGT]GG
SfcI 855 CTACAG C^T[GA][CT]AG
SspBI 818 TGTACA T^GTACA
StuI 695 AGGCCT AGG^CCT
TseI 334 GCTGC G^C[AT]GC
XhoII 598 GGATCC [GA]^GATC[CT]
XmnI 137 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcsI 3 GAATTC [GA]^AATT[CT]
137 GAATTT
ApoI 3 GAATTC [GA]^AATT[CT]
137 GAATTT
AvaII 496 GGACC G^G[AT]CC
746 GGACC
BmyI 685 GGGCAC G[AGT]GC[ACT]^C
884 GGGCTC
BsaJI 270 CCTTGG C^C[ACGT][ACGT]GG
338 CCATGG
Bsp1286I 685 GGGCAC G[AGT]GC[ACT]^C
884 GGGCTC
BspHI 92 TCATGA T^CATGA
921 TCATGA
Cfr13I 496 GGACC G^G[ACGT]CC
746 GGACC
EcoT14I 270 CCTTGG C^C[AT][AT]GG
338 CCATGG
Fnu4HI 298 GCGGC GC^[ACGT]GC
334 GCTGC
HphI 828 GGTGA GGTGA(8/7)
656 TCACC
ItaI 298 GCGGC GC^[ACGT]GC
334 GCTGC
MseI 58 TTAA T^TAA
878 TTAA
RcaI 92 TCATGA T^CATGA
921 TCATGA
Sau96I 496 GGACC G^G[ACGT]CC
746 GGACC
StyI 270 CCTTGG C^C[AT][AT]GG
338 CCATGG
TaqI 78 TCGA T^CGA
317 TCGA
TfiI 21 GAATC G^A[AT]TC
831 GATTC
Tru9I 58 TTAA T^TAA
878 TTAA
Tsp45I 217 GTCAC ^GT[GC]AC
613 GTCAC
TthHB8I 78 TCGA T^CGA
317 TCGA
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AluI AlwI BsiYI BslI
BsmAI BstF5I DdeI DpnII EarI
FokI HaeIII HinfI Ksp632I MaeIII
MboI MboII MnlI MslI MwoI
NdeII NlaIII NlaIV Sau3AI SfaNI
Tsp509I TspRI