Restriction map for
pFN21AE6035
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanII BbeI BbrPI BbsI
BcgI BclI BcnI BfrI BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstF5I BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FokI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MamI MfeI MluI MroI MseI
MspA1I MspI MunI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tru9I
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AcsI 31 GAATTT [GA]^AATT[CT]
AfaI 180 GTAC GT^AC
AlwNI 936 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 31 GAATTT [GA]^AATT[CT]
Asp700I 525 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 723 GTGCTC G[AT]GC[AT]^C
BalI 374 TGGCCA TGG^CCA
BanI 511 GGCACC G^G[CT][GA]CC
BbvCI 903 CCTCAGC CCTCAGC(-5/-2)
BciVI 944 GTATCC GTATCC(6/5)
BglI 427 GCCAAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 590 AGATCT A^GATCT
BpmI 208 CTGGAG CTGGAG(16/14)
BsaAI 720 TACGTG [CT]AC^GT[GA]
BseRI 726 CTCCTC GAGGAG(10/8)
BsiHKAI 723 GTGCTC G[AT]GC[AT]^C
BsmAI 745 GTCTC GTCTC(1/5)
BstEII 144 GGTTACC G^GT[ACGT]ACC
BstPI 144 GGTTACC G^GT[ACGT]ACC
DsaI 353 CCATGG C^C[GA][CT]GG
EaeI 374 TGGCCA [CT]^GGCC[GA]
EcoO65I 144 GGTTACC G^GT[ACGT]ACC
FauI 546 CCCGC CCCGC(4/6)
HinfI 846 GACTC G^A[ACGT]TC
MaeII 721 ACGT A^CGT
MluNI 374 TGGCCA TGG^CCA
MscI 374 TGGCCA TGG^CCA
NcoI 353 CCATGG C^CATGG
NdeI 359 CATATG CA^TATG
PflMI 502 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 846 GACTC GAGTC(4/5)
PstI 381 CTGCAG CTGCA^G
RsaI 180 GTAC GT^AC
Van91I 502 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 525 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 366 CCGC CCGC(-3/-1)
547 CCGC
AlwI 627 GATCC GGATC(4/5)
684 GATCC
BmyI 235 GTGCCC G[AGT]GC[ACT]^C
723 GTGCTC
BsgI 475 GTGCAG GTGCAG(16/14)
569 GTGCAG
BsmFI 250 GGGAC GGGAC(10/14)
386 GTCCC
BsmI 334 GAATGC GAATGC(1/-1)
897 GAATGC
Bsp1286I 235 GTGCCC G[AGT]GC[ACT]^C
723 GTGCTC
BsrDI 77 CATTGC GCAATG(2/0)
663 CATTGC
BstXI 239 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
611 CCATTCTGGTGG
BstYI 590 AGATCT [GA]^GATC[CT]
683 AGATCC
EcoT14I 285 CCTTGG C^C[AT][AT]GG
353 CCATGG
MflI 590 AGATCT [GA]^GATC[CT]
683 AGATCC
SapI 81 GCTCTTC GCTCTTC(1/4)
489 GCTCTTC
SmlI 679 CTCAAG C^T[CT][GA]AG
876 CTTGAG
StyI 285 CCTTGG C^C[AT][AT]GG
353 CCATGG
Tsp45I 539 GTGAC ^GT[GC]AC
844 GTGAC
Tsp509I 32 AATT ^AATT
799 AATT
TspRI 581 CACTG CA[GC]TG[ACGT][ACGT]^
837 CACTG
XhoII 590 AGATCT [GA]^GATC[CT]
683 AGATCC
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AluI AvaII BbvI BfaI BsaJI
BsiYI BslI BspHI BsrI BssKI
BstNI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII Fnu4HI
HaeIII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MslI
MvaI MwoI NdeII NlaIII NlaIV
PpuMI RcaI Sau3AI Sau96I ScrFI
SfcI TseI