Restriction map for
pFN21AB7577
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BbrPI BbsI BcgI BciVI BclI
BfaI BfrI BglII BlnI BpuAI
BsaBI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstXI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FokI FseI FspI HgaI
HincII HindII HindIII HinfI HpaI
KpnI Ksp632I MaeI MamI MfeI
MflI MluI MluNI MroI MscI
MseI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmlI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI TfiI Tru9I Tth111I TthHB8I
Van91I XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AcsI 481 AAATTC [GA]^AATT[CT]
AlwI 387 GATCC GGATC(4/5)
ApaI 63 GGGCCC GGGCC^C
ApoI 481 AAATTC [GA]^AATT[CT]
BanII 63 GGGCCC G[GA]GC[CT]^C
BbvCI 609 CCTCAGC CCTCAGC(-5/-2)
BglI 49 GCCGCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 214 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 214 GCTGAGC GC^T[ACGT]AGC
BsaAI 289 TACGTA [CT]AC^GT[GA]
BsaI 78 GGTCTC GGTCTC(1/5)
BsaWI 234 TCCGGT [AT]^CCGG[AT]
BsmBI 314 GAGACG CGTCTC(1/5)
BsrBI 217 GAGCGG CCGCTC(-3/-3)
BsrFI 39 GCCGGC [GA]^CCGG[CT]
BsrI 403 ACTGG ACTGG(1/-1)
BstNI 154 CCTGG CC^[AT]GG
CelII 214 GCTGAGC GC^T[ACGT]AGC
Cfr10I 39 GCCGGC [GA]^CCGG[CT]
DpnII 387 GATC ^GATC
EaeI 305 CGGCCA [CT]^GGCC[GA]
EcoRII 154 CCTGG ^CC[AT]GG
HphI 650 TCACC GGTGA(8/7)
MboI 387 GATC ^GATC
MslI 624 CATCAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 154 CCTGG CC^[AT]GG
NaeI 39 GCCGGC GCC^GGC
NcoI 587 CCATGG C^CATGG
NdeII 387 GATC ^GATC
NgoMIV 39 GCCGGC G^CCGGC
NlaIII 588 CATG CATG^
PstI 6 CTGCAG CTGCA^G
Sau3AI 387 GATC ^GATC
SmaI 60 CCCGGG CCC^GGG
SnaBI 289 TACGTA TAC^GTA
StuI 434 AGGCCT AGG^CCT
Tsp45I 400 GTGAC ^GT[GC]AC
XcmI 335 CCAGCACGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 60 CCCGGG C^CCGGG
XmaI 60 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 36 GGCGCC G[GA]^CG[CT]C
255 GGCGCC
AfaI 292 GTAC GT^AC
576 GTAC
AlwNI 167 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
398 CAGTGACTG
AvaI 28 CCCGAG C^[CT]CG[GA]G
60 CCCGGG
BanI 36 GGCGCC G^G[CT][GA]CC
255 GGCGCC
BbeI 36 GGCGCC GGCGC^C
255 GGCGCC
BpmI 102 CTCCAG CTGGAG(16/14)
161 CTGGAG
BsaHI 36 GGCGCC G[GA]^CG[CT]C
255 GGCGCC
BsmAI 314 GAGAC GTCTC(1/5)
79 GTCTC
BsoBI 28 CCCGAG C^[CT]CG[GA]G
60 CCCGGG
EcoT14I 188 CCTTGG C^C[AT][AT]GG
587 CCATGG
HaeII 36 GGCGCC [GA]GCGC^[CT]
255 GGCGCC
Hin1I 36 GGCGCC G[GA]^CG[CT]C
255 GGCGCC
KasI 36 GGCGCC G^GCGCC
255 GGCGCC
KspI 113 CCGCGG CCGC^GG
134 CCGCGG
MaeII 290 ACGT A^CGT
317 ACGT
MaeIII 400 GTGAC ^GT[ACGT]AC
407 GTTAC
MboII 370 GAAGA GAAGA(8/7)
633 GAAGA
NarI 36 GGCGCC GG^CGCC
255 GGCGCC
PpuMI 150 GGGACCT [GA]G^G[AT]CC[CT]
183 GGGACCC
RsaI 292 GTAC GT^AC
576 GTAC
SacII 113 CCGCGG CCGC^GG
134 CCGCGG
SfcI 6 CTGCAG C^T[GA][CT]AG
511 CTACAG
SfoI 36 GGCGCC GGC^GCC
255 GGCGCC
StyI 188 CCTTGG C^C[AT][AT]GG
587 CCATGG
Tsp509I 453 AATT ^AATT
482 AATT
TspRI 398 CAGTG CA[GC]TG[ACGT][ACGT]^
517 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AccII AciI AluI AvaII BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BssKI BstUI Cac8I
CfoI Cfr13I DdeI DraII DsaI
EcoO109I FauI Fnu4HI HaeIII HapII
HhaI HinP1I HpaII ItaI MnlI
MspA1I MspI MvnI MwoI NciI
NlaIV PvuII Sau96I ScrFI SfaNI
TseI