Restriction map for
pFN21AE5912
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BcnI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoT22I FauI FseI FspI
HaeII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeIII MamI MfeI MluI
MroI MspA1I MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tsp45I
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AciI 369 CCGC CCGC(-3/-1)
AlwI 71 GGATC GGATC(4/5)
AvaI 407 CCCGAG C^[CT]CG[GA]G
BalI 311 TGGCCA TGG^CCA
BclI 147 TGATCA T^GATCA
BsaWI 68 ACCGGA [AT]^CCGG[AT]
BsiYI 490 CCATATTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 490 CCATATTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 715 GTCTC GTCTC(1/5)
BsmI 392 GCATTC GAATGC(1/-1)
BsoBI 407 CCCGAG C^[CT]CG[GA]G
BstXI 230 CCACAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 71 GGATCT [GA]^GATC[CT]
EaeI 311 TGGCCA [CT]^GGCC[GA]
EarI 125 CTCTTC CTCTTC(1/4)
EcoRV 562 GATATC GAT^ATC
EcoT14I 204 CCTTGG C^C[AT][AT]GG
FbaI 147 TGATCA T^GATCA
HapII 69 CCGG C^CGG
HgaI 676 GCGTC GACGC(5/10)
HpaII 69 CCGG C^CGG
HphI 287 TCACC GGTGA(8/7)
Ksp632I 125 CTCTTC CTCTTC(1/4)
MaeII 568 ACGT A^CGT
MboII 126 TCTTC GAAGA(8/7)
MflI 71 GGATCT [GA]^GATC[CT]
MluNI 311 TGGCCA TGG^CCA
MscI 311 TGGCCA TGG^CCA
MseI 572 TTAA T^TAA
MspI 69 CCGG C^CGG
NspI 789 ACATGC [GA]CATG^[CT]
PflMI 490 CCATATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SmlI 655 CTCAAG C^T[CT][GA]AG
StyI 204 CCTTGG C^C[AT][AT]GG
Tru9I 572 TTAA T^TAA
Van91I 490 CCATATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 71 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
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AvaII 201 GGACC G^G[AT]CC
804 GGTCC
BanII 48 GGGCTC G[GA]GC[CT]^C
163 GAGCCC
BbvI 249 GCTGC GCAGC(8/12)
277 GCTGC
BfaI 253 CTAG C^TAG
736 CTAG
BsaJI 204 CCTTGG C^C[ACGT][ACGT]GG
237 CCTGGG
BsmFI 759 GGGAC GGGAC(10/14)
805 GTCCC
BsrDI 360 CATTGC GCAATG(2/0)
672 CATTGC
BssKI 180 CCTGG ^CC[ACGT]GG
237 CCTGG
BstF5I 366 CATCC GGATG(2/0)
209 GGATG
BstNI 180 CCTGG CC^[AT]GG
237 CCTGG
Cac8I 19 GCTGGC GC[ACGT]^[ACGT]GC
189 GCTGGC
DdeI 51 CTCAG C^T[ACGT]AG
379 CTGAG
DpnII 72 GATC ^GATC
148 GATC
EcoRII 180 CCTGG ^CC[AT]GG
237 CCTGG
Fnu4HI 249 GCTGC GC^[ACGT]GC
277 GCTGC
FokI 366 CATCC GGATG(9/13)
209 GGATG
ItaI 249 GCTGC GC^[ACGT]GC
277 GCTGC
MaeI 253 CTAG C^TAG
736 CTAG
MboI 72 GATC ^GATC
148 GATC
MvaI 180 CCTGG CC^[AT]GG
237 CCTGG
NdeII 72 GATC ^GATC
148 GATC
Sau3AI 72 GATC ^GATC
148 GATC
ScrFI 180 CCTGG CC^[ACGT]GG
237 CCTGG
SfaNI 619 GATGC GCATC(5/9)
869 GCATC
TseI 249 GCTGC G^C[AT]GC
277 GCTGC
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# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AfaI AluI BmyI Bsp1286I BsrI
Cfr13I HaeIII HinfI MnlI MslI
MwoI NlaIII NlaIV RsaI Sau96I
TfiI Tsp509I TspRI