Restriction map for
pFN21AE1679
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# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BalI BamHI BbsI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BpuAI BsaBI BseAI BseRI BsgI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrGI
BsrI BssSI Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
ClaI CpoI DpnII DraI DraII
DraIII DrdI Eam1105I EarI Eco47III
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
HincII HindII HindIII HpaI KpnI
Ksp632I MaeI MaeIII MamI MboI
MfeI MflI MluI MluNI MroI
MscI MseI MslI MunI NcoI
NdeI NdeII NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI Sau3AI
SbfI ScaI SexAI SfaNI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TfiI Tru9I Tsp45I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AfaI 291 GTAC GT^AC
AspHI 322 GAGCAC G[AT]GC[AT]^C
AvaI 447 CCCGGG C^[CT]CG[GA]G
AviII 335 TGCGCA TGC^GCA
BanI 241 GGCGCC G^G[CT][GA]CC
BanII 469 GAGCCC G[GA]GC[CT]^C
BbeI 241 GGCGCC GGCGC^C
BbrPI 226 CACGTG CAC^GTG
BbvCI 357 GCTGAGG CCTCAGC(-5/-2)
BpmI 558 CTCCAG CTGGAG(16/14)
BsaAI 226 CACGTG [CT]AC^GT[GA]
BsaWI 133 ACCGGA [AT]^CCGG[AT]
BsiHKAI 322 GAGCAC G[AT]GC[AT]^C
BsmBI 268 GAGACG CGTCTC(1/5)
BsoBI 447 CCCGGG C^[CT]CG[GA]G
BsrDI 164 GCAATG GCAATG(2/0)
BstF5I 294 CATCC GGATG(2/0)
FokI 294 CATCC GGATG(9/13)
FspI 335 TGCGCA TGC^GCA
HinfI 528 GAGTC G^A[ACGT]TC
HphI 195 GGTGA GGTGA(8/7)
KasI 241 GGCGCC G^GCGCC
MaeII 227 ACGT A^CGT
MboII 439 TCTTC GAAGA(8/7)
NaeI 104 GCCGGC GCC^GGC
NarI 241 GGCGCC GG^CGCC
NgoMIV 104 GCCGGC G^CCGGC
PleI 528 GAGTC GAGTC(4/5)
PmaCI 226 CACGTG CAC^GTG
PmlI 226 CACGTG CAC^GTG
PshAI 74 GACCCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 561 CAGCTG CAG^CTG
RsaI 291 GTAC GT^AC
SfoI 241 GGCGCC GGC^GCC
SmaI 447 CCCGGG CCC^GGG
TaqI 551 TCGA T^CGA
TspRI 14 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 551 TCGA T^CGA
XmaCI 447 CCCGGG C^CCGGG
XmaI 447 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 241 GGCGCC G[GA]^CG[CT]C
328 GACGCC
AvaII 23 GGTCC G^G[AT]CC
404 GGACC
BglI 387 GCCCCGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
452 GCCGCGGGGGC
BlpI 255 GCTGAGC GC^T[ACGT]AGC
278 GCTCAGC
BmyI 322 GAGCAC G[AGT]GC[ACT]^C
469 GAGCCC
Bpu1102I 255 GCTGAGC GC^T[ACGT]AGC
278 GCTCAGC
BsaHI 241 GGCGCC G[GA]^CG[CT]C
328 GACGCC
BsaI 72 GAGACC GGTCTC(1/5)
130 GAGACC
Bsp1286I 322 GAGCAC G[AGT]GC[ACT]^C
469 GAGCCC
BsrFI 104 GCCGGC [GA]^CCGG[CT]
117 ACCGGC
BssHII 299 GCGCGC G^CGCGC
521 GCGCGC
CelII 255 GCTGAGC GC^T[ACGT]AGC
278 GCTCAGC
Cfr10I 104 GCCGGC [GA]^CCGG[CT]
117 ACCGGC
EagI 149 CGGCCG C^GGCCG
376 CGGCCG
EclXI 149 CGGCCG C^GGCCG
376 CGGCCG
Eco52I 149 CGGCCG C^GGCCG
376 CGGCCG
Hin1I 241 GGCGCC G[GA]^CG[CT]C
328 GACGCC
KspI 390 CCGCGG CCGC^GG
453 CCGCGG
PstI 99 CTGCAG CTGCA^G
304 CTGCAG
SacII 390 CCGCGG CCGC^GG
453 CCGCGG
SfcI 99 CTGCAG C^T[GA][CT]AG
304 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AccII AciI AluI AlwNI BbvI
BcnI BsaJI BsiEI BsiYI BslI
BsmAI BsmFI BssKI BstAPI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DsaI EaeI EcoRII FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
HinP1I HpaII ItaI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIV Sau96I ScrFI TseI