Restriction map for
pFN21AE6639
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmI
Bsp1407I BspEI BspHI BspLU11I BspMI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MaeII MaeIII MamI
MfeI MluI MroI MseI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp45I Tsp509I Tth111I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AfaI 36 GTAC GT^AC
AlwI 117 GATCC GGATC(4/5)
AvaI 488 CCCGGG C^[CT]CG[GA]G
AvaII 713 GGACC G^G[AT]CC
BalI 449 TGGCCA TGG^CCA
BcgI 294 GCAGAGGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsmBI 519 GAGACG CGTCTC(1/5)
BsoBI 488 CCCGGG C^[CT]CG[GA]G
BspDI 538 ATCGAT AT^CGAT
BsrI 336 ACTGG ACTGG(1/-1)
BstYI 116 AGATCC [GA]^GATC[CT]
ClaI 538 ATCGAT AT^CGAT
DsaI 422 CCATGG C^C[GA][CT]GG
EaeI 449 TGGCCA [CT]^GGCC[GA]
EcoNI 447 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 737 GCGGG CCCGC(4/6)
MflI 116 AGATCC [GA]^GATC[CT]
MluNI 449 TGGCCA TGG^CCA
MscI 449 TGGCCA TGG^CCA
MslI 544 CACCTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 601 CAGCTG C[AC]G^C[GT]G
NcoI 422 CCATGG C^CATGG
NlaIII 423 CATG CATG^
PpuMI 712 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 601 CAGCTG CAG^CTG
RsaI 36 GTAC GT^AC
SfaNI 370 GATGC GCATC(5/9)
SfcI 435 CTACAG C^T[GA][CT]AG
SmaI 488 CCCGGG CCC^GGG
TspRI 105 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 116 AGATCC [GA]^GATC[CT]
XmaCI 488 CCCGGG C^CCGGG
XmaI 488 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AspHI 184 GAGCTC G[AT]GC[AT]^C
580 GAGCTC
BanII 184 GAGCTC G[GA]GC[CT]^C
580 GAGCTC
BbsI 553 GAAGAC GAAGAC(2/6)
417 GTCTTC
BbvI 291 GCAGC GCAGC(8/12)
440 GCAGC
BmyI 184 GAGCTC G[AGT]GC[ACT]^C
580 GAGCTC
BpmI 187 CTCCAG CTGGAG(16/14)
604 CTGGAG
BpuAI 553 GAAGAC GAAGAC(2/6)
417 GTCTTC
BsaI 93 GAGACC GGTCTC(1/5)
589 GAGACC
BsiHKAI 184 GAGCTC G[AT]GC[AT]^C
580 GAGCTC
BsmFI 566 GGGAC GGGAC(10/14)
712 GGGAC
Bsp1286I 184 GAGCTC G[AGT]GC[ACT]^C
580 GAGCTC
BsrBI 529 CCGCTC CCGCTC(-3/-3)
284 GAGCGG
BstF5I 150 CATCC GGATG(2/0)
369 GGATG
Cac8I 524 GCAAGC GC[ACGT]^[ACGT]GC
737 GCGGGC
DpnII 117 GATC ^GATC
541 GATC
DraII 191 AGGCCCT [GA]G^G[ACGT]CC[CT]
712 GGGACCC
EcoO109I 191 AGGCCCT [GA]G^G[ACGT]CC[CT]
712 GGGACCC
FokI 150 CATCC GGATG(9/13)
369 GGATG
HapII 219 CCGG C^CGG
489 CCGG
HgaI 521 GACGC GACGC(5/10)
162 GCGTC
HpaII 219 CCGG C^CGG
489 CCGG
MboI 117 GATC ^GATC
541 GATC
MspI 219 CCGG C^CGG
489 CCGG
MwoI 277 GCAGAGAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
728 GCAAAGTCGGC
NdeII 117 GATC ^GATC
541 GATC
SacI 184 GAGCTC GAGCT^C
580 GAGCTC
Sau3AI 117 GATC ^GATC
541 GATC
TseI 291 GCAGC G^C[AT]GC
440 GCAGC
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI BcnI BsaJI BseRI
BsgI BsiYI BslI BsmAI BssKI
BstNI CfoI Cfr13I DdeI EarI
EcoRII EcoT14I Fnu4HI HaeII HaeIII
HhaI HinP1I HinfI HphI ItaI
Ksp632I MboII MnlI MvaI NciI
NlaIV Sau96I ScrFI StyI TaqI
TfiI TthHB8I