Restriction map for
pFN21AE2513
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspEI BspHI BspLU11I BspMI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MaeII MaeIII MamI MfeI MluI
MroI MseI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp45I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccII 99 CGCG CG^CG
AfaI 36 GTAC GT^AC
AlwI 141 GATCC GGATC(4/5)
AvaI 512 CCCGGG C^[CT]CG[GA]G
AvaII 737 GGACC G^G[AT]CC
BalI 473 TGGCCA TGG^CCA
BcgI 318 GCAGAGGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsmBI 543 GAGACG CGTCTC(1/5)
BsoBI 512 CCCGGG C^[CT]CG[GA]G
BspDI 562 ATCGAT AT^CGAT
BsrI 360 ACTGG ACTGG(1/-1)
BstUI 99 CGCG CG^CG
BstYI 140 AGATCC [GA]^GATC[CT]
ClaI 562 ATCGAT AT^CGAT
DsaI 446 CCATGG C^C[GA][CT]GG
EaeI 473 TGGCCA [CT]^GGCC[GA]
EcoNI 471 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 761 GCGGG CCCGC(4/6)
MflI 140 AGATCC [GA]^GATC[CT]
MluNI 473 TGGCCA TGG^CCA
MscI 473 TGGCCA TGG^CCA
MslI 568 CACCTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 625 CAGCTG C[AC]G^C[GT]G
MvnI 99 CGCG CG^CG
NcoI 446 CCATGG C^CATGG
NlaIII 447 CATG CATG^
PpuMI 736 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 625 CAGCTG CAG^CTG
RsaI 36 GTAC GT^AC
SfaNI 394 GATGC GCATC(5/9)
SfcI 459 CTACAG C^T[GA][CT]AG
SmaI 512 CCCGGG CCC^GGG
TspRI 129 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 140 AGATCC [GA]^GATC[CT]
XmaCI 512 CCCGGG C^CCGGG
XmaI 512 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AspHI 208 GAGCTC G[AT]GC[AT]^C
604 GAGCTC
BanII 208 GAGCTC G[GA]GC[CT]^C
604 GAGCTC
BbvI 315 GCAGC GCAGC(8/12)
464 GCAGC
BmyI 208 GAGCTC G[AGT]GC[ACT]^C
604 GAGCTC
BpmI 211 CTCCAG CTGGAG(16/14)
628 CTGGAG
BsaI 117 GAGACC GGTCTC(1/5)
613 GAGACC
BsiHKAI 208 GAGCTC G[AT]GC[AT]^C
604 GAGCTC
BsmFI 590 GGGAC GGGAC(10/14)
736 GGGAC
Bsp1286I 208 GAGCTC G[AGT]GC[ACT]^C
604 GAGCTC
BsrBI 553 CCGCTC CCGCTC(-3/-3)
308 GAGCGG
BstF5I 174 CATCC GGATG(2/0)
393 GGATG
Cac8I 548 GCAAGC GC[ACGT]^[ACGT]GC
761 GCGGGC
DpnII 141 GATC ^GATC
565 GATC
DraII 215 AGGCCCT [GA]G^G[ACGT]CC[CT]
736 GGGACCC
EcoO109I 215 AGGCCCT [GA]G^G[ACGT]CC[CT]
736 GGGACCC
FokI 174 CATCC GGATG(9/13)
393 GGATG
HapII 243 CCGG C^CGG
513 CCGG
HpaII 243 CCGG C^CGG
513 CCGG
MboI 141 GATC ^GATC
565 GATC
MspI 243 CCGG C^CGG
513 CCGG
MwoI 301 GCAGAGAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
752 GCAAAGTCGGC
NdeII 141 GATC ^GATC
565 GATC
SacI 208 GAGCTC GAGCT^C
604 GAGCTC
Sau3AI 141 GATC ^GATC
565 GATC
TseI 315 GCAGC G^C[AT]GC
464 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI BbsI BcnI BpuAI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BssKI BstNI CfoI Cfr13I
DdeI EarI EcoRII EcoT14I Fnu4HI
HaeII HaeIII HgaI HhaI HinP1I
HinfI HphI ItaI Ksp632I MboII
MnlI MvaI NciI NlaIV Sau96I
ScrFI StyI TaqI TfiI TthHB8I