Restriction map for
pFN21AE5532
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BlnI BsaAI BsaBI BsaHI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I ClaI CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MluNI MscI MseI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacI SacII SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SspBI SspI SwaI
TfiI Tru9I Tsp509I Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccIII 479 TCCGGA T^CCGGA
AhdI 856 GACCCGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 856 GACCCGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1018 GAGCAC G[AT]GC[AT]^C
BanII 892 GGGCTC G[GA]GC[CT]^C
BbsI 1063 GTCTTC GAAGAC(2/6)
BglII 314 AGATCT A^GATCT
BpuAI 1063 GTCTTC GAAGAC(2/6)
BsaI 1128 GGTCTC GGTCTC(1/5)
BsaWI 479 TCCGGA [AT]^CCGG[AT]
BseAI 479 TCCGGA T^CCGGA
BsiHKAI 1018 GAGCAC G[AT]GC[AT]^C
BsmBI 342 GAGACG CGTCTC(1/5)
BspEI 479 TCCGGA T^CCGGA
BspHI 773 TCATGA T^CATGA
BsrFI 129 GCCGGT [GA]^CCGG[CT]
BstYI 314 AGATCT [GA]^GATC[CT]
Cfr10I 129 GCCGGT [GA]^CCGG[CT]
DpnII 315 GATC ^GATC
Eam1105I 856 GACCCGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 450 CCTGTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 681 CCCGC CCCGC(4/6)
HinfI 111 GAGTC G^A[ACGT]TC
MboI 315 GATC ^GATC
MflI 314 AGATCT [GA]^GATC[CT]
MroI 479 TCCGGA T^CCGGA
NdeII 315 GATC ^GATC
PleI 111 GAGTC GAGTC(4/5)
PpuMI 848 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 525 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 773 TCATGA T^CATGA
SapI 1038 GAAGAGC GCTCTTC(1/4)
Sau3AI 315 GATC ^GATC
SbfI 1086 CCTGCAGG CCTGCA^GG
SmlI 184 CTTGAG C^T[CT][GA]AG
SphI 417 GCATGC GCATG^C
Sse8387I 1086 CCTGCAGG CCTGCA^GG
StuI 1010 AGGCCT AGG^CCT
XcmI 549 CCAGCATTTCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 314 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 604 CGCG CG^CG
918 CGCG
BlpI 516 GCTGAGC GC^T[ACGT]AGC
591 GCTAAGC
BmyI 892 GGGCTC G[AGT]GC[ACT]^C
1018 GAGCAC
BpmI 103 CTGGAG CTGGAG(16/14)
1099 CTGGAG
Bpu1102I 516 GCTGAGC GC^T[ACGT]AGC
591 GCTAAGC
BseRI 19 CTCCTC GAGGAG(10/8)
511 GAGGAG
BsgI 430 CTGCAC GTGCAG(16/14)
646 CTGCAC
BsmAI 342 GAGAC GTCTC(1/5)
1129 GTCTC
Bsp1286I 892 GGGCTC G[AGT]GC[ACT]^C
1018 GAGCAC
BspMI 1172 ACCTGC ACCTGC(4/8)
1089 GCAGGT
BsrI 243 ACTGG ACTGG(1/-1)
461 ACTGG
BstAPI 194 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
432 GCACATAGTGC
BstUI 604 CGCG CG^CG
918 CGCG
CelII 516 GCTGAGC GC^T[ACGT]AGC
591 GCTAAGC
CfoI 603 GCGC GCG^C
796 GCGC
DraIII 379 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG
433 CACATAGTG
EaeI 348 TGGCCG [CT]^GGCC[GA]
635 TGGCCG
HgaI 96 GACGC GACGC(5/10)
842 GCGTC
HhaI 603 GCGC GCG^C
796 GCGC
HinP1I 603 GCGC G^CGC
796 GCGC
MslI 553 CATTTCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
615 CATCCACATG
MvnI 604 CGCG CG^CG
918 CGCG
NcoI 259 CCATGG C^CATGG
270 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaI
AvaII BbvI BcnI BsaJI BsiYI
BslI BsmFI BsoBI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DraII
DsaI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboII MnlI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV NspI
PflMI PstI PvuII RsaI Sau96I
ScrFI SfaNI SfcI SmaI StyI
TaqI TseI Tsp45I TspRI TthHB8I
Van91I XmaCI XmaI