Restriction map for
pFN21AE9618
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaLI
ApoI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BciVI
BclI BfrI BglI BlnI BpuAI
BsaAI BsaBI BsaHI BsaI BseRI
BsiEI BsiWI BsmAI BsmBI BspLU11I
BsrBI BsrDI BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HpaI KasI KpnI MamI
MfeI MluI MslI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SmaI SnaBI SpeI SphI
SspI SwaI Tsp509I Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 236 TCCGGA T^CCGGA
AlwNI 402 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 1005 GAGCTC G[AT]GC[AT]^C
BalI 584 TGGCCA TGG^CCA
BanI 222 GGTGCC G^G[CT][GA]CC
BcgI 132 CGATGTGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1117 CTAG C^TAG
BglII 936 AGATCT A^GATCT
BlpI 385 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 385 GCTGAGC GC^T[ACGT]AGC
BsaWI 236 TCCGGA [AT]^CCGG[AT]
BseAI 236 TCCGGA T^CCGGA
BsiHKAI 1005 GAGCTC G[AT]GC[AT]^C
Bsp1407I 491 TGTACA T^GTACA
BspDI 688 ATCGAT AT^CGAT
BspEI 236 TCCGGA T^CCGGA
BspHI 648 TCATGA T^CATGA
BspMI 763 ACCTGC ACCTGC(4/8)
BsrGI 491 TGTACA T^GTACA
BstAPI 767 GCACAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 936 AGATCT [GA]^GATC[CT]
CelII 385 GCTGAGC GC^T[ACGT]AGC
ClaI 688 ATCGAT AT^CGAT
EaeI 584 TGGCCA [CT]^GGCC[GA]
EarI 1141 GAAGAG CTCTTC(1/4)
HinfI 393 GATTC G^A[ACGT]TC
HphI 172 GGTGA GGTGA(8/7)
Ksp632I 1141 GAAGAG CTCTTC(1/4)
MaeI 1117 CTAG C^TAG
MflI 936 AGATCT [GA]^GATC[CT]
MluNI 584 TGGCCA TGG^CCA
MroI 236 TCCGGA T^CCGGA
MscI 584 TGGCCA TGG^CCA
MseI 284 TTAA T^TAA
NaeI 436 GCCGGC GCC^GGC
NgoMIV 436 GCCGGC G^CCGGC
NspI 912 GCATGT [GA]CATG^[CT]
PflMI 411 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 373 CTGCAG CTGCA^G
PvuII 1084 CAGCTG CAG^CTG
RcaI 648 TCATGA T^CATGA
SacI 1005 GAGCTC GAGCT^C
SbfI 372 CCTGCAGG CCTGCA^GG
SfcI 373 CTGCAG C^T[GA][CT]AG
SgrAI 849 CACCGGCG C[GA]^CCGG[CT]G
SmlI 697 CTTGAG C^T[CT][GA]AG
Sse8387I 372 CCTGCAGG CCTGCA^GG
SspBI 491 TGTACA T^GTACA
TfiI 393 GATTC G^A[AT]TC
Tru9I 284 TTAA T^TAA
Van91I 411 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 936 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
ApaI 164 GGGCCC GGGCC^C
1045 GGGCCC
AscI 17 GGCGCGCC GG^CGCGCC
189 GGCGCGCC
AvaI 217 CCCGAG C^[CT]CG[GA]G
526 CCCGAG
BpmI 502 CTCCAG CTGGAG(16/14)
964 CTGGAG
BsmI 232 GCATTC GAATGC(1/-1)
458 GCATTC
BsoBI 217 CCCGAG C^[CT]CG[GA]G
526 CCCGAG
BsrFI 436 GCCGGC [GA]^CCGG[CT]
850 ACCGGC
BssHII 18 GCGCGC G^CGCGC
190 GCGCGC
Cfr10I 436 GCCGGC [GA]^CCGG[CT]
850 ACCGGC
DraIII 449 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1102 CACGGCGTG
EcoNI 197 CCTTGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
407 CCTGCCAGAGG
HindIII 364 AAGCTT A^AGCTT
1177 AAGCTT
KspI 23 CCGCGG CCGC^GG
168 CCGCGG
MaeIII 353 GTGAC ^GT[ACGT]AC
622 GTCAC
NcoI 159 CCATGG C^CATGG
929 CCATGG
PpuMI 955 GGGACCT [GA]G^G[AT]CC[CT]
1151 GGGACCT
SacII 23 CCGCGG CCGC^GG
168 CCGCGG
StuI 95 AGGCCT AGG^CCT
205 AGGCCT
Tsp45I 353 GTGAC ^GT[GC]AC
622 GTCAC
TspRI 351 CAGTG CA[GC]TG[ACGT][ACGT]^
453 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BanII BbvI BcnI BmyI BsaJI
BsgI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI MaeII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TseI
TthHB8I