Restriction map for
pFN21AB7566
##################################
# NO CUT ENZYMES ( 138 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaLI
ApoI AseI AsnI Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BlnI BsaAI
BsaBI BsaI BsaWI BseAI BseRI
BsiHKAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BsrI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
Eam1105I EarI Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I MaeI MaeIII MamI
MfeI MluI MluNI MroI MscI
MseI MslI MunI NcoI NdeI
NheI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tru9I Tsp45I Tsp509I Tth111I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 78 GACGTC GACGT^C
AfaI 513 GTAC GT^AC
AscI 370 GGCGCGCC GG^CGCGCC
Asp700I 492 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 75 GAAGAC GAAGAC(2/6)
BglII 476 AGATCT A^GATCT
BlpI 30 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 30 GCTGAGC GC^T[ACGT]AGC
BpuAI 75 GAAGAC GAAGAC(2/6)
BsmAI 539 GTCTC GTCTC(1/5)
BsmBI 538 CGTCTC CGTCTC(1/5)
BsrBI 157 CCGCTC CCGCTC(-3/-3)
BstF5I 186 CATCC GGATG(2/0)
BstYI 476 AGATCT [GA]^GATC[CT]
CelII 30 GCTGAGC GC^T[ACGT]AGC
DdeI 31 CTGAG C^T[ACGT]AG
EcoNI 423 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 186 CATCC GGATG(9/13)
FseI 193 GGCCGGCC GGCCGG^CC
HinfI 655 GACTC G^A[ACGT]TC
HphI 573 GGTGA GGTGA(8/7)
KspI 284 CCGCGG CCGC^GG
MaeII 79 ACGT A^CGT
MflI 476 AGATCT [GA]^GATC[CT]
NotI 286 GCGGCCGC GC^GGCCGC
PflMI 344 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 655 GACTC GAGTC(4/5)
PshAI 385 GACCCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 100 CTGCAG CTGCA^G
RsaI 513 GTAC GT^AC
SacII 284 CCGCGG CCGC^GG
SfaNI 176 GCATC GCATC(5/9)
SfcI 100 CTGCAG C^T[GA][CT]AG
SgrAI 264 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 15 CCCGGG CCC^GGG
SmlI 763 CTCAAG C^T[CT][GA]AG
TspRI 36 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 344 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 476 AGATCT [GA]^GATC[CT]
XmaCI 15 CCCGGG C^CCGGG
XmaI 15 CCCGGG C^CCGGG
XmnI 492 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
ApaI 280 GGGCCC GGGCC^C
522 GGGCCC
BanI 123 GGCGCC G^G[CT][GA]CC
335 GGCGCC
BbeI 123 GGCGCC GGCGC^C
335 GGCGCC
BpmI 378 CTGGAG CTGGAG(16/14)
487 CTGGAG
BsgI 55 CTGCAC GTGCAG(16/14)
364 GTGCAG
BstXI 311 CCAAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
509 CCAAGTACCTGG
DpnII 477 GATC ^GATC
750 GATC
DsaI 200 CCGTGG C^C[GA][CT]GG
284 CCGCGG
KasI 123 GGCGCC G^GCGCC
335 GGCGCC
MboI 477 GATC ^GATC
750 GATC
NarI 123 GGCGCC GG^CGCC
335 GGCGCC
NdeII 477 GATC ^GATC
750 GATC
NlaIII 63 CATG CATG^
558 CATG
PpuMI 163 GGGACCC [GA]G^G[AT]CC[CT]
383 GGGACCC
Sau3AI 477 GATC ^GATC
750 GATC
SfoI 123 GGCGCC GGC^GCC
335 GGCGCC
TaqI 500 TCGA T^CGA
781 TCGA
TthHB8I 500 TCGA T^CGA
781 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
AvaII BanII BbvI BcnI BglI
BmyI BsaHI BsaJI BsiEI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrFI
BssHII BssKI BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DraII EaeI
EagI EclXI Eco52I EcoO109I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
ItaI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NgoMIV NlaIV Sau96I ScrFI TseI