Restriction map for
pFN21AE5065
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AlwNI Aor51HI AscI AseI
AsnI Asp700I AviII AvrII BcgI
BciVI BclI BfaI BfrI BlnI
BsaBI BsaWI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI Bst1107I BstAPI
BstXI BstZ17I ClaI CpoI DraI
DraIII DrdI EarI Eco47III EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI Ksp632I MaeI MamI
MfeI MluI MroI MslI MunI
NdeI NheI NruI NsiI NspI
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PstI PvuI RcaI RsrII
SalI SapI SbfI ScaI SexAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 1076 GGTACC G^GTACC
AcsI 1645 GAATTT [GA]^AATT[CT]
Alw44I 817 GTGCAC G^TGCAC
AocI 1231 CCTGAGG CC^T[ACGT]AGG
ApaLI 817 GTGCAC G^TGCAC
ApoI 1645 GAATTT [GA]^AATT[CT]
Asp718I 1076 GGTACC G^GTACC
BalI 1344 TGGCCA TGG^CCA
BamHI 101 GGATCC G^GATCC
BbrPI 1726 CACGTG CAC^GTG
BbsI 1140 GTCTTC GAAGAC(2/6)
BbvCI 495 CCTCAGC CCTCAGC(-5/-2)
BglII 761 AGATCT A^GATCT
BlpI 552 GCTGAGC GC^T[ACGT]AGC
BpmI 829 CTGGAG CTGGAG(16/14)
Bpu1102I 552 GCTGAGC GC^T[ACGT]AGC
BpuAI 1140 GTCTTC GAAGAC(2/6)
BstBI 361 TTCGAA TT^CGAA
BstEII 1330 GGTGACC G^GT[ACGT]ACC
BstPI 1330 GGTGACC G^GT[ACGT]ACC
Bsu36I 1231 CCTGAGG CC^T[ACGT]AGG
CelII 552 GCTGAGC GC^T[ACGT]AGC
Eco81I 1231 CCTGAGG CC^T[ACGT]AGG
EcoNI 1281 CCTGGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1330 GGTGACC G^GT[ACGT]ACC
KpnI 1076 GGTACC GGTAC^C
MluNI 1344 TGGCCA TGG^CCA
MscI 1344 TGGCCA TGG^CCA
NotI 513 GCGGCCGC GC^GGCCGC
NspV 361 TTCGAA TT^CGAA
PflMI 1011 CCACCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1726 CACGTG CAC^GTG
PmlI 1726 CACGTG CAC^GTG
PshAI 1528 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfcI 768 CTACAG C^T[GA][CT]AG
SfiI 130 GGCCTCTTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 361 TTCGAA TT^CGAA
SgrAI 1094 CGCCGGCG C[GA]^CCGG[CT]G
Tsp509I 1646 AATT ^AATT
TspRI 1254 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 1011 CCACCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AflIII 401 ACACGT A^C[GA][CT]GT
1725 ACACGT
AhdI 892 GACAGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
907 GACCGGCCGTC
ApaI 596 GGGCCC GGGCC^C
722 GGGCCC
AspEI 892 GACAGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
907 GACCGGCCGTC
AspI 1474 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1602 GACTACGTC
BglI 131 GCCTCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1560 GCCGTCTTGGC
BsaAI 402 CACGTA [CT]AC^GT[GA]
1726 CACGTG
BsaI 283 GAGACC GGTCTC(1/5)
1581 GGTCTC
BsrBI 85 GAGCGG CCGCTC(-3/-3)
1241 GAGCGG
BsrI 1253 CCAGT ACTGG(1/-1)
1758 CCAGT
Eam1105I 892 GACAGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
907 GACCGGCCGTC
HincII 49 GTCAAC GT[CT]^[GA]AC
184 GTCAAC
HindII 49 GTCAAC GT[CT]^[GA]AC
184 GTCAAC
KspI 264 CCGCGG CCGC^GG
742 CCGCGG
MaeIII 67 GTCAC ^GT[ACGT]AC
1331 GTGAC
MboII 1674 GAAGA GAAGA(8/7)
1141 TCTTC
MseI 1664 TTAA T^TAA
1753 TTAA
NcoI 195 CCATGG C^CATGG
1494 CCATGG
NlaIII 196 CATG CATG^
1495 CATG
PflFI 1474 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1602 GACTACGTC
PpuMI 737 AGGTCCC [GA]G^G[AT]CC[CT]
875 AGGACCC
PvuII 235 CAGCTG CAG^CTG
1224 CAGCTG
SacI 631 GAGCTC GAGCT^C
1747 GAGCTC
SacII 264 CCGCGG CCGC^GG
742 CCGCGG
SmaI 480 CCCGGG CCC^GGG
902 CCCGGG
TaqI 170 TCGA T^CGA
362 TCGA
Tru9I 1664 TTAA T^TAA
1753 TTAA
Tsp45I 67 GTCAC ^GT[GC]AC
1331 GTGAC
Tth111I 1474 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1602 GACTACGTC
TthHB8I 170 TCGA T^CGA
362 TCGA
XmaCI 480 CCCGGG C^CCGGG
902 CCCGGG
XmaI 480 CCCGGG C^CCGGG
902 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI AvaII BanI
BanII BbeI BbvI BcnI BmyI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BssHII
BssKI BssSI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeII MboI MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfoI StyI TseI
XhoII