Restriction map for
pFN21AE3126
##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BbrPI BbsI BcgI BciVI BclI
BfaI BfrI BlnI BpuAI BsaBI
BsaI BseAI BsgI BsiHKAI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI Eam1105I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HinfI HpaI KpnI MaeI MamI
MfeI MluI MroI MunI NdeI
NheI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SalI SapI ScaI SexAI
SfaNI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI SspBI SspI
SwaI TaqI TfiI TspRI Tth111I
TthHB8I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AfaI 390 GTAC GT^AC
AlwI 90 GGATC GGATC(4/5)
AlwNI 283 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
BbvCI 291 GCTGAGG CCTCAGC(-5/-2)
BglII 380 AGATCT A^GATCT
BpmI 658 CTGGAG CTGGAG(16/14)
BsaAI 391 TACGTG [CT]AC^GT[GA]
BsaWI 736 TCCGGT [AT]^CCGG[AT]
BsrBI 217 CCGCTC CCGCTC(-3/-3)
BsrFI 904 GCCGGC [GA]^CCGG[CT]
BssHII 350 GCGCGC G^CGCGC
BstAPI 282 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 626 CCAGGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 904 GCCGGC [GA]^CCGG[CT]
HgaI 369 GACGC GACGC(5/10)
KspI 896 CCGCGG CCGC^GG
MaeII 392 ACGT A^CGT
MseI 860 TTAA T^TAA
MslI 330 CATCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 904 GCCGGC GCC^GGC
NcoI 335 CCATGG C^CATGG
NgoMIV 904 GCCGGC G^CCGGC
NlaIII 336 CATG CATG^
PflMI 387 CCAGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 165 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 688 CTGCAG CTGCA^G
RsaI 390 GTAC GT^AC
SacII 896 CCGCGG CCGC^GG
SbfI 687 CCTGCAGG CCTGCA^GG
Sse8387I 687 CCTGCAGG CCTGCA^GG
StuI 692 AGGCCT AGG^CCT
Tru9I 860 TTAA T^TAA
Tsp45I 920 GTCAC ^GT[GC]AC
Tsp509I 520 AATT ^AATT
Van91I 387 CCAGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 387 CCAGTACGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
ApaI 24 GGGCCC GGGCC^C
111 GGGCCC
BsmFI 943 GGGAC GGGAC(10/14)
167 GTCCC
BspMI 686 ACCTGC ACCTGC(4/8)
727 ACCTGC
BsrI 524 ACTGG ACTGG(1/-1)
387 CCAGT
BstYI 90 GGATCT [GA]^GATC[CT]
380 AGATCT
DpnII 91 GATC ^GATC
381 GATC
DsaI 335 CCATGG C^C[GA][CT]GG
896 CCGCGG
EarI 579 GAAGAG CTCTTC(1/4)
585 GAAGAG
EcoT14I 335 CCATGG C^C[AT][AT]GG
774 CCTTGG
HphI 126 GGTGA GGTGA(8/7)
921 TCACC
Ksp632I 579 GAAGAG CTCTTC(1/4)
585 GAAGAG
MaeIII 401 GTAAC ^GT[ACGT]AC
920 GTCAC
MboI 91 GATC ^GATC
381 GATC
MflI 90 GGATCT [GA]^GATC[CT]
380 AGATCT
MspA1I 355 CCGCTG C[AC]G^C[GT]G
896 CCGCGG
NdeII 91 GATC ^GATC
381 GATC
NotI 303 GCGGCCGC GC^GGCCGC
898 GCGGCCGC
Sau3AI 91 GATC ^GATC
381 GATC
SfcI 688 CTGCAG C^T[GA][CT]AG
930 CTACAG
StyI 335 CCATGG C^C[AT][AT]GG
774 CCTTGG
XhoII 90 GGATCT [GA]^GATC[CT]
380 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AcyI AluI Aor51HI
AvaI AvaII BalI BanI BanII
BbeI BbvI BcnI BglI BlpI
BmyI Bpu1102I BsaHI BsaJI BseRI
BsiEI BsiYI BslI BsoBI Bsp1286I
BssKI BstF5I BstNI BstUI Cac8I
CelII CfoI Cfr13I DdeI DraII
EaeI EagI EclXI Eco47III Eco52I
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII ItaI KasI MboII
MluNI MnlI MscI MspI MvaI
MvnI MwoI NarI NciI NlaIV
Sau96I ScrFI SfoI SmaI TseI
XmaCI XmaI