Restriction map for
pFN21AE6762
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI Alw44I AocI ApaLI AscI
Asp718I AspI AviII AvrII BbvCI
BfaI BfrI BlnI BlpI Bpu1102I
BsaBI BsiEI BsiWI Bsp1407I BspDI
BspLU11I BsrFI BsrGI BssSI Bst1107I
BstAPI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DrdI
EagI EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MaeI MamI MfeI MluI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI
PflFI PinAI PmeI PshAI Psp1406I
PvuI RsrII SacII SalI SapI
SfiI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 285 TCCGGA T^CCGGA
AcsI 1019 GAATTT [GA]^AATT[CT]
AfaI 1197 GTAC GT^AC
AhdI 1406 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1045 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1019 GAATTT [GA]^AATT[CT]
AseI 520 ATTAAT AT^TAAT
AsnI 520 ATTAAT AT^TAAT
Asp700I 1782 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1406 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2168 TGGCCA TGG^CCA
BamHI 2174 GGATCC G^GATCC
BbsI 1602 GAAGAC GAAGAC(2/6)
BcgI 1914 GCAGGTTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1572 GTATCC GTATCC(6/5)
BclI 363 TGATCA T^GATCA
BpuAI 1602 GAAGAC GAAGAC(2/6)
BsaWI 285 TCCGGA [AT]^CCGG[AT]
BseAI 285 TCCGGA T^CCGGA
BsgI 1177 GTGCAG GTGCAG(16/14)
BsmI 1209 GAATGC GAATGC(1/-1)
BspEI 285 TCCGGA T^CCGGA
BspHI 1682 TCATGA T^CATGA
BsrBI 208 GAGCGG CCGCTC(-3/-3)
BstBI 1813 TTCGAA TT^CGAA
BstXI 1823 CCATCTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 1374 TTTAAA TTT^AAA
Eam1105I 1406 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2250 GAAGAG CTCTTC(1/4)
EcoT14I 794 CCATGG C^C[AT][AT]GG
FbaI 363 TGATCA T^GATCA
Ksp632I 2250 GAAGAG CTCTTC(1/4)
MluNI 2168 TGGCCA TGG^CCA
MroI 285 TCCGGA T^CCGGA
MscI 2168 TGGCCA TGG^CCA
NcoI 794 CCATGG C^CATGG
NspV 1813 TTCGAA TT^CGAA
PleI 500 GACTC GAGTC(4/5)
PpuMI 92 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 520 ATTAAT AT^TAAT
PstI 1546 CTGCAG CTGCA^G
PvuII 1861 CAGCTG CAG^CTG
RcaI 1682 TCATGA T^CATGA
RsaI 1197 GTAC GT^AC
SbfI 1545 CCTGCAGG CCTGCA^GG
ScaI 1196 AGTACT AGT^ACT
SfcI 1546 CTGCAG C^T[GA][CT]AG
SfuI 1813 TTCGAA TT^CGAA
Sse8387I 1545 CCTGCAGG CCTGCA^GG
StuI 1286 AGGCCT AGG^CCT
StyI 794 CCATGG C^C[AT][AT]GG
XmnI 1782 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 701 ACACGT A^C[GA][CT]GT
1637 ACGTGT
Aor51HI 27 AGCGCT AGC^GCT
470 AGCGCT
ApaI 190 GGGCCC GGGCC^C
1130 GGGCCC
BbrPI 702 CACGTG CAC^GTG
1636 CACGTG
BglII 116 AGATCT A^GATCT
1070 AGATCT
BsaI 163 GAGACC GGTCTC(1/5)
1489 GAGACC
BsmBI 1237 GAGACG CGTCTC(1/5)
2253 GAGACG
BspMI 1544 ACCTGC ACCTGC(4/8)
1914 GCAGGT
BsrDI 689 GCAATG GCAATG(2/0)
1139 GCAATG
BssHII 142 GCGCGC G^CGCGC
1213 GCGCGC
DraIII 1225 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
2133 CACAAGGTG
EaeI 59 TGGCCG [CT]^GGCC[GA]
2168 TGGCCA
Eco47III 27 AGCGCT AGC^GCT
470 AGCGCT
EcoNI 295 CCTCCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1227 CCTGGTGGAGG
PaeR7I 88 CTCGAG C^TCGAG
160 CTCGAG
PflMI 409 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1224 CCACCTGGTGG
PmaCI 702 CACGTG CAC^GTG
1636 CACGTG
PmlI 702 CACGTG CAC^GTG
1636 CACGTG
SacI 157 GAGCTC GAGCT^C
339 GAGCTC
SmaI 9 CCCGGG CCC^GGG
193 CCCGGG
Van91I 409 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1224 CCACCTGGTGG
XcmI 929 CCACTGATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2099 CCAAGAACTGTATGG
XhoI 88 CTCGAG C^TCGAG
160 CTCGAG
XmaCI 9 CCCGGG C^CCGGG
193 CCCGGG
XmaI 9 CCCGGG C^CCGGG
193 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AspHI AvaI AvaII BanI BanII
BbeI BbvI BcnI BglI BmyI
BpmI BsaAI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SexAI SfaNI
SfoI SmlI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII