Restriction map for
pFN21AE9434
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# NO CUT ENZYMES ( 134 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AlwI
AlwNI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BbvCI BciVI BfrI
BglII BlnI BsaBI BsaI BsaWI
BseAI BsiWI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FseI
FspI HindIII HpaI KpnI KspI
MaeIII MamI MfeI MflI MluI
MluNI MroI MscI MseI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfuI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tru9I Tsp45I
Tsp509I Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 26 enzymes) #
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AflIII 386 ACACGT A^C[GA][CT]GT
BanII 790 GAGCTC G[GA]GC[CT]^C
BcgI 608 GCAAGCGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 342 CCGGG CC^[GC]GG
BfaI 784 CTAG C^TAG
BglI 965 GCCTCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 685 CTGGAG CTGGAG(16/14)
BsmAI 52 GTCTC GTCTC(1/5)
BsmBI 51 CGTCTC CGTCTC(1/5)
BsrBI 181 CCGCTC CCGCTC(-3/-3)
BsrDI 604 CATTGC GCAATG(2/0)
DraII 742 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 468 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 742 GGGACCT [GA]G^G[ACGT]CC[CT]
HincII 172 GTCAAC GT[CT]^[GA]AC
HindII 172 GTCAAC GT[CT]^[GA]AC
MaeI 784 CTAG C^TAG
NciI 342 CCGGG CC^[GC]GG
NspI 176 ACATGC [GA]CATG^[CT]
PleI 976 GACTC GAGTC(4/5)
PpuMI 742 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 451 CAGCTG CAG^CTG
SacI 790 GAGCTC GAGCT^C
SgrAI 119 CGCCGGCG C[GA]^CCGG[CT]G
TfiI 921 GATTC G^A[AT]TC
XcmI 278 CCATGCTCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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Alw44I 162 GTGCAC G^TGCAC
465 GTGCAC
ApaLI 162 GTGCAC G^TGCAC
465 GTGCAC
AvaII 654 GGACC G^G[AT]CC
743 GGACC
BbeI 117 GGCGCC GGCGC^C
520 GGCGCC
BbrPI 144 CACGTG CAC^GTG
387 CACGTG
BbsI 325 GTCTTC GAAGAC(2/6)
511 GTCTTC
BclI 623 TGATCA T^GATCA
773 TGATCA
BpuAI 325 GTCTTC GAAGAC(2/6)
511 GTCTTC
BseRI 957 CTCCTC GAGGAG(10/8)
688 GAGGAG
BsiEI 225 CGACCG CG[GA][CT]^CG
306 CGACCG
BsmFI 11 GGGAC GGGAC(10/14)
742 GGGAC
BspMI 155 ACCTGC ACCTGC(4/8)
656 ACCTGC
BstAPI 467 GCACGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
905 GCAAAAGCTGC
EarI 127 CTCTTC CTCTTC(1/4)
793 CTCTTC
FbaI 623 TGATCA T^GATCA
773 TGATCA
HinfI 921 GATTC G^A[ACGT]TC
976 GACTC
KasI 117 GGCGCC G^GCGCC
520 GGCGCC
Ksp632I 127 CTCTTC CTCTTC(1/4)
793 CTCTTC
MslI 615 CATCGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
661 CACCCCAGTG
NarI 117 GGCGCC GG^CGCC
520 GGCGCC
PmaCI 144 CACGTG CAC^GTG
387 CACGTG
PmlI 144 CACGTG CAC^GTG
387 CACGTG
SapI 126 GCTCTTC GCTCTTC(1/4)
792 GCTCTTC
SfoI 117 GGCGCC GGC^GCC
520 GGCGCC
SmlI 577 CTCAAG C^T[CT][GA]AG
586 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI BanI BbvI BlpI BmyI
Bpu1102I BsaAI BsaHI BsaJI BsgI
BsiHKAI BsiYI BslI Bsp1286I BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DsaI
EaeI EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII HphI ItaI
MaeII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NaeI
NdeII NgoMIV NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TseI TspRI TthHB8I