Restriction map for
pFN21AE3137
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BamHI BanII BbrPI
BciVI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaWI BseAI BseRI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FseI
FspI HincII HindII HindIII HpaI
HphI KpnI MfeI MroI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StyI SwaI
Tth111I Van91I XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AatII 169 GACGTC GACGT^C
AccI 487 GTCTAC GT^[AC][GT]AC
AfaI 255 GTAC GT^AC
AflIII 182 ACGCGT A^C[GA][CT]GT
AseI 283 ATTAAT AT^TAAT
AsnI 283 ATTAAT AT^TAAT
AspHI 1035 GAGCAC G[AT]GC[AT]^C
BalI 570 TGGCCA TGG^CCA
BanI 213 GGCGCC G^G[CT][GA]CC
BbeI 213 GGCGCC GGCGC^C
BbsI 3 GAAGAC GAAGAC(2/6)
BbvCI 760 GCTGAGG CCTCAGC(-5/-2)
BclI 828 TGATCA T^GATCA
BcnI 194 CCGGG CC^[GC]GG
BglII 363 AGATCT A^GATCT
BmyI 1035 GAGCAC G[AGT]GC[ACT]^C
BpmI 945 CTGGAG CTGGAG(16/14)
BpuAI 3 GAAGAC GAAGAC(2/6)
BsaBI 358 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 978 GTGCAG GTGCAG(16/14)
BsiEI 208 CGACCG CG[GA][CT]^CG
BsiHKAI 1035 GAGCAC G[AT]GC[AT]^C
BsmFI 304 GTCCC GGGAC(10/14)
Bsp1286I 1035 GAGCAC G[AGT]GC[ACT]^C
BspMI 346 ACCTGC ACCTGC(4/8)
BsrDI 962 GCAATG GCAATG(2/0)
BstXI 217 CCATCCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 363 AGATCT [GA]^GATC[CT]
EaeI 570 TGGCCA [CT]^GGCC[GA]
EarI 1023 GAAGAG CTCTTC(1/4)
FauI 79 CCCGC CCCGC(4/6)
FbaI 828 TGATCA T^GATCA
HaeII 213 GGCGCC [GA]GCGC^[CT]
KasI 213 GGCGCC G^GCGCC
Ksp632I 1023 GAAGAG CTCTTC(1/4)
MaeII 170 ACGT A^CGT
MaeIII 715 GTGAC ^GT[ACGT]AC
MamI 358 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 363 AGATCT [GA]^GATC[CT]
MluI 182 ACGCGT A^CGCGT
MluNI 570 TGGCCA TGG^CCA
MscI 570 TGGCCA TGG^CCA
MslI 218 CATCCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 213 GGCGCC GG^CGCC
NciI 194 CCGGG CC^[GC]GG
NspI 688 GCATGT [GA]CATG^[CT]
PleI 681 GAGTC GAGTC(4/5)
PshBI 283 ATTAAT AT^TAAT
PvuII 493 CAGCTG CAG^CTG
RsaI 255 GTAC GT^AC
SfaNI 612 GATGC GCATC(5/9)
SfoI 213 GGCGCC GGC^GCC
SmlI 547 CTTGAG C^T[CT][GA]AG
SspI 506 AATATT AAT^ATT
StuI 54 AGGCCT AGG^CCT
TfiI 1016 GAATC G^A[AT]TC
Tsp45I 715 GTGAC ^GT[GC]AC
XbaI 427 TCTAGA T^CTAGA
XhoII 363 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 169 GACGTC G[GA]^CG[CT]C
213 GGCGCC
AlwI 357 GGATC GGATC(4/5)
950 GGATC
AlwNI 241 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
583 CAGGCACTG
BsaHI 169 GACGTC G[GA]^CG[CT]C
213 GGCGCC
BsaI 550 GAGACC GGTCTC(1/5)
598 GAGACC
BsiYI 75 CCTCCCCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
79 CCCGCGGCAGG
BslI 75 CCTCCCCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
79 CCCGCGGCAGG
BsmAI 550 GAGAC GTCTC(1/5)
598 GAGAC
BsrI 393 CCAGT ACTGG(1/-1)
748 CCAGT
BssHII 103 GCGCGC G^CGCGC
105 GCGCGC
BstNI 258 CCTGG CC^[AT]GG
708 CCTGG
DsaI 43 CCGCGG C^C[GA][CT]GG
80 CCGCGG
EcoRII 258 CCTGG ^CC[AT]GG
708 CCTGG
HgaI 181 GACGC GACGC(5/10)
62 GCGTC
Hin1I 169 GACGTC G[GA]^CG[CT]C
213 GGCGCC
HinfI 681 GAGTC G^A[ACGT]TC
1016 GAATC
KspI 43 CCGCGG CCGC^GG
80 CCGCGG
MvaI 258 CCTGG CC^[AT]GG
708 CCTGG
NlaIV 25 GGAACC GG[ACGT]^[ACGT]CC
213 GGCGCC
PstI 124 CTGCAG CTGCA^G
377 CTGCAG
SacII 43 CCGCGG CCGC^GG
80 CCGCGG
SfcI 124 CTGCAG C^T[GA][CT]AG
377 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccII AciI AluI BbvI BcgI
BfaI BsaJI BsrFI BssKI BstF5I
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII ItaI
MaeI MboI MboII MnlI MseI
MspA1I MspI MvnI MwoI NdeII
NlaIII Sau3AI Sau96I ScrFI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I