Restriction map for
pFN21AE0290
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# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BpuAI BsaBI BsaI BsaWI BseAI
BsiHKAI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BsrI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstYI
BstZ17I Bsu36I ClaI CpoI DpnII
DraI DraIII DrdI Eam1105I EarI
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FokI FseI FspI
HincII HindII HindIII HinfI HpaI
KpnI Ksp632I MaeI MaeIII MamI
MboI MboII MfeI MflI MluI
MluNI MroI MscI MseI MslI
MunI NdeI NdeII NheI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmeI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SalI SapI
Sau3AI SbfI SexAI SfaNI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tru9I Tsp45I TspRI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AfaI 789 GTAC GT^AC
AlwNI 120 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 437 AGCGCT AGC^GCT
AvaII 350 GGTCC G^G[AT]CC
BbrPI 741 CACGTG CAC^GTG
BlpI 450 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 450 GCTGAGC GC^T[ACGT]AGC
BsgI 583 CTGCAC GTGCAG(16/14)
BspMI 633 GCAGGT ACCTGC(4/8)
BsrFI 291 GCCGGC [GA]^CCGG[CT]
BssSI 64 CTCGTG CACGAG(-5/-1)
BstXI 161 CCAAAGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 450 GCTGAGC GC^T[ACGT]AGC
Cfr10I 291 GCCGGC [GA]^CCGG[CT]
DdeI 451 CTGAG C^T[ACGT]AG
Eco47III 437 AGCGCT AGC^GCT
EcoT14I 56 CCATGG C^C[AT][AT]GG
HgaI 537 GACGC GACGC(5/10)
HphI 158 TCACC GGTGA(8/7)
NaeI 291 GCCGGC GCC^GGC
NcoI 56 CCATGG C^CATGG
NgoMIV 291 GCCGGC G^CCGGC
NlaIII 57 CATG CATG^
PmaCI 741 CACGTG CAC^GTG
PmlI 741 CACGTG CAC^GTG
PpuMI 349 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 631 CTGCAG CTGCA^G
RsaI 789 GTAC GT^AC
ScaI 788 AGTACT AGT^ACT
SfcI 631 CTGCAG C^T[GA][CT]AG
SmlI 694 CTCAAG C^T[CT][GA]AG
StyI 56 CCATGG C^C[AT][AT]GG
Tsp509I 383 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AflIII 740 ACACGT A^C[GA][CT]GT
742 ACGTGT
BanI 18 GGCGCC G^G[CT][GA]CC
757 GGCGCC
BbeI 18 GGCGCC GGCGC^C
757 GGCGCC
BglI 592 GCCTCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
854 GCCCGCCTGGC
BpmI 394 CTGGAG CTGGAG(16/14)
430 CTGGAG
BsaAI 82 TACGTG [CT]AC^GT[GA]
741 CACGTG
KasI 18 GGCGCC G^GCGCC
757 GGCGCC
KspI 36 CCGCGG CCGC^GG
198 CCGCGG
MaeII 83 ACGT A^CGT
742 ACGT
NarI 18 GGCGCC GG^CGCC
757 GGCGCC
NotI 38 GCGGCCGC GC^GGCCGC
604 GCGGCCGC
SacII 36 CCGCGG CCGC^GG
198 CCGCGG
SfoI 18 GGCGCC GGC^GCC
757 GGCGCC
SmaI 93 CCCGGG CCC^GGG
353 CCCGGG
XmaCI 93 CCCGGG C^CCGGG
353 CCCGGG
XmaI 93 CCCGGG C^CCGGG
353 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AcyI AluI ApaI
AvaI BanII BbvI BcnI BmyI
BsaHI BsaJI BseRI BsiEI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrBI
BssHII BssKI BstNI BstUI Cac8I
CfoI Cfr13I DraII DsaI EaeI
EagI EclXI Eco52I EcoO109I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HhaI Hin1I HinP1I HpaII ItaI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIV Sau96I ScrFI
TseI