Restriction map for
pFN21AE1734
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII Hin1I HincII HindII HpaI
KasI KpnI Ksp632I KspI MaeII
MfeI MluNI MroI MscI MslI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SphI Sse8387I SspBI SspI SwaI
TspRI Tth111I Van91I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 577 GTAGAC GT^[AC][GT]AC
AciI 72 CCGC CCGC(-3/-1)
AfaI 273 GTAC GT^AC
AflIII 112 ACGCGT A^C[GA][CT]GT
AocI 700 CCTGAGG CC^T[ACGT]AGG
Asp700I 430 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 679 GAGCTC G[AT]GC[AT]^C
AvaI 45 CCCGGG C^[CT]CG[GA]G
AvaII 54 GGTCC G^G[AT]CC
BbvI 610 GCAGC GCAGC(8/12)
BglI 42 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 69 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 460 GAGGAG GAGGAG(10/8)
BsiHKAI 679 GAGCTC G[AT]GC[AT]^C
BsiYI 45 CCCGGGGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 45 CCCGGGGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 634 GTCTC GTCTC(1/5)
BsmFI 55 GTCCC GGGAC(10/14)
BsoBI 45 CCCGGG C^[CT]CG[GA]G
BsrDI 556 GCAATG GCAATG(2/0)
BsrFI 51 GCCGGT [GA]^CCGG[CT]
BssHII 38 GCGCGC G^CGCGC
BstXI 17 CCAGGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 624 AGATCC [GA]^GATC[CT]
Bsu36I 700 CCTGAGG CC^T[ACGT]AGG
Cfr10I 51 GCCGGT [GA]^CCGG[CT]
Eco81I 700 CCTGAGG CC^T[ACGT]AGG
Fnu4HI 610 GCAGC GC^[ACGT]GC
HphI 263 TCACC GGTGA(8/7)
ItaI 610 GCAGC GC^[ACGT]GC
MaeIII 710 GTGAC ^GT[ACGT]AC
MamI 69 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 624 AGATCC [GA]^GATC[CT]
MluI 112 ACGCGT A^CGCGT
MspA1I 241 CAGCTG C[AC]G^C[GT]G
PvuII 241 CAGCTG CAG^CTG
RsaI 273 GTAC GT^AC
SacI 679 GAGCTC GAGCT^C
SfaNI 74 GCATC GCATC(5/9)
SfcI 78 CTACAG C^T[GA][CT]AG
SmaI 45 CCCGGG CCC^GGG
SpeI 649 ACTAGT A^CTAGT
StuI 704 AGGCCT AGG^CCT
TaqI 67 TCGA T^CGA
TfiI 137 GAATC G^A[AT]TC
TseI 610 GCAGC G^C[AT]GC
Tsp45I 710 GTGAC ^GT[GC]AC
TthHB8I 67 TCGA T^CGA
XhoII 624 AGATCC [GA]^GATC[CT]
XmaCI 45 CCCGGG C^CCGGG
XmaI 45 CCCGGG C^CCGGG
XmnI 430 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccII 39 CGCG CG^CG
113 CGCG
AcsI 433 GAATTT [GA]^AATT[CT]
445 GAATTT
AlwI 69 GATCC GGATC(4/5)
625 GATCC
ApoI 433 GAATTT [GA]^AATT[CT]
445 GAATTT
BanI 213 GGTGCC G^G[CT][GA]CC
366 GGTGCC
BbsI 409 GAAGAC GAAGAC(2/6)
480 GAAGAC
BpuAI 409 GAAGAC GAAGAC(2/6)
480 GAAGAC
BsrI 662 ACTGG ACTGG(1/-1)
217 CCAGT
BstUI 39 CGCG CG^CG
113 CGCG
Cac8I 38 GCGCGC GC[ACGT]^[ACGT]GC
331 GCTTGC
CfoI 38 GCGC GCG^C
40 GCGC
EcoT14I 131 CCAAGG C^C[AT][AT]GG
374 CCTTGG
HgaI 111 GACGC GACGC(5/10)
114 GCGTC
HhaI 38 GCGC GCG^C
40 GCGC
HinP1I 38 GCGC G^CGC
40 GCGC
HindIII 329 AAGCTT A^AGCTT
384 AAGCTT
MseI 191 TTAA T^TAA
565 TTAA
MvnI 39 CGCG CG^CG
113 CGCG
PleI 10 GAGTC GAGTC(4/5)
253 GAGTC
StyI 131 CCAAGG C^C[AT][AT]GG
374 CCTTGG
Tru9I 191 TTAA T^TAA
565 TTAA
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AluI BanII BcnI BfaI BmyI
BsaJI Bsp1286I BssKI BstF5I BstNI
Cfr13I DdeI DpnII EcoRII FokI
HaeIII HapII HinfI HpaII MaeI
MboI MboII MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI Tsp509I