Restriction map for
pFN21AE6357
##################################
# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BseRI BsiEI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoRI EcoRV EcoT22I FauI FseI
FspI HgaI Hin1I KasI KpnI
KspI MaeI MaeII MamI MfeI
MluI MunI MvnI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SspBI SspI StuI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 955 GTCTAC GT^[AC][GT]AC
AccIII 249 TCCGGA T^CCGGA
AfaI 65 GTAC GT^AC
Asp700I 768 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 345 GAAGAC GAAGAC(2/6)
BclI 746 TGATCA T^GATCA
BpuAI 345 GAAGAC GAAGAC(2/6)
BsaWI 249 TCCGGA [AT]^CCGG[AT]
BseAI 249 TCCGGA T^CCGGA
BsgI 160 GTGCAG GTGCAG(16/14)
BspEI 249 TCCGGA T^CCGGA
BsrFI 175 GCCGGC [GA]^CCGG[CT]
BstEII 183 GGTGACC G^GT[ACGT]ACC
BstPI 183 GGTGACC G^GT[ACGT]ACC
BstXI 488 CCACGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 923 AGATCC [GA]^GATC[CT]
CfoI 783 GCGC GCG^C
Cfr10I 175 GCCGGC [GA]^CCGG[CT]
EarI 876 GAAGAG CTCTTC(1/4)
EcoO65I 183 GGTGACC G^GT[ACGT]ACC
FbaI 746 TGATCA T^GATCA
HaeII 782 AGCGCC [GA]GCGC^[CT]
HhaI 783 GCGC GCG^C
HinP1I 783 GCGC G^CGC
HincII 400 GTTAAC GT[CT]^[GA]AC
HindII 400 GTTAAC GT[CT]^[GA]AC
HindIII 143 AAGCTT A^AGCTT
HpaI 400 GTTAAC GTT^AAC
Ksp632I 876 GAAGAG CTCTTC(1/4)
MflI 923 AGATCC [GA]^GATC[CT]
MroI 249 TCCGGA T^CCGGA
MseI 401 TTAA T^TAA
NaeI 175 GCCGGC GCC^GGC
NcoI 728 CCATGG C^CATGG
NgoMIV 175 GCCGGC G^CCGGC
RsaI 65 GTAC GT^AC
SapI 876 GAAGAGC GCTCTTC(1/4)
SbfI 807 CCTGCAGG CCTGCA^GG
ScaI 64 AGTACT AGT^ACT
SphI 326 GCATGC GCATG^C
Sse8387I 807 CCTGCAGG CCTGCA^GG
TaqI 111 TCGA T^CGA
Tru9I 401 TTAA T^TAA
Tsp509I 20 AATT ^AATT
TthHB8I 111 TCGA T^CGA
XhoII 923 AGATCC [GA]^GATC[CT]
XmnI 768 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AspHI 879 GAGCTC G[AT]GC[AT]^C
917 GAGCTC
BalI 485 TGGCCA TGG^CCA
731 TGGCCA
BanI 297 GGTGCC G^G[CT][GA]CC
801 GGCACC
BpmI 476 CTGGAG CTGGAG(16/14)
760 CTGGAG
BsiHKAI 879 GAGCTC G[AT]GC[AT]^C
917 GAGCTC
Cac8I 175 GCCGGC GC[ACGT]^[ACGT]GC
326 GCATGC
DdeI 913 CTGAG C^T[ACGT]AG
920 CTCAG
EcoT14I 530 CCTTGG C^C[AT][AT]GG
728 CCATGG
MluNI 485 TGGCCA TGG^CCA
731 TGGCCA
MscI 485 TGGCCA TGG^CCA
731 TGGCCA
NspI 284 GCATGT [GA]CATG^[CT]
326 GCATGC
PleI 235 GACTC GAGTC(4/5)
500 GACTC
SacI 879 GAGCTC GAGCT^C
917 GAGCTC
SfaNI 578 GCATC GCATC(5/9)
620 GCATC
StyI 530 CCTTGG C^C[AT][AT]GG
728 CCATGG
XcmI 473 CCACTGGAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
941 CCAGCGTTGAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BbvI BcnI BmyI BsaJI BsiYI
BslI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cfr13I DpnII
DsaI EaeI EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PstI
PvuII Sau3AI Sau96I ScrFI SfcI
TfiI TseI Tsp45I TspRI