Restriction map for
pFN21AE5342
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AfaI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaII AviII
AvrII BalI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BclI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI Bsp1286I Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI Hin1I HpaI KasI KpnI
Ksp632I KspI MaeII MamI MfeI
MluNI MroI MscI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PstI PvuI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 678 GTCTAC GT^[AC][GT]AC
AflIII 501 ACGCGT A^C[GA][CT]GT
AvaI 690 CTCGGG C^[CT]CG[GA]G
BamHI 632 GGATCC G^GATCC
BciVI 41 GGATAC GTATCC(6/5)
BcnI 50 CCGGG CC^[GC]GG
BfaI 683 CTAG C^TAG
BpmI 472 CTGGAG CTGGAG(16/14)
BsaI 475 GAGACC GGTCTC(1/5)
BsmFI 393 GTCCC GGGAC(10/14)
BsoBI 690 CTCGGG C^[CT]CG[GA]G
BspHI 453 TCATGA T^CATGA
BstBI 704 TTCGAA TT^CGAA
Cac8I 570 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 37 GCGC GCG^C
Cfr13I 14 GGGCC G^G[ACGT]CC
DraII 13 GGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 247 CGGCCA [CT]^GGCC[GA]
EcoO109I 13 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 658 GAATTC G^AATTC
HhaI 37 GCGC GCG^C
HinP1I 37 GCGC G^CGC
HincII 507 GTTGAC GT[CT]^[GA]AC
HindII 507 GTTGAC GT[CT]^[GA]AC
HindIII 319 AAGCTT A^AGCTT
MaeI 683 CTAG C^TAG
MboII 361 GAAGA GAAGA(8/7)
MluI 501 ACGCGT A^CGCGT
MseI 484 TTAA T^TAA
MslI 810 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 110 CAGCTG C[AC]G^C[GT]G
NciI 50 CCGGG CC^[GC]GG
NcoI 722 CCATGG C^CATGG
NspV 704 TTCGAA TT^CGAA
PflMI 598 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 304 GACATTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 110 CAGCTG CAG^CTG
RcaI 453 TCATGA T^CATGA
Sau96I 14 GGGCC G^G[ACGT]CC
SfcI 653 CTACAG C^T[GA][CT]AG
SfuI 704 TTCGAA TT^CGAA
SmlI 637 CTTGAG C^T[CT][GA]AG
SspI 749 AATATT AAT^ATT
TaqI 705 TCGA T^CGA
Tru9I 484 TTAA T^TAA
TthHB8I 705 TCGA T^CGA
Van91I 598 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccII 38 CGCG CG^CG
502 CGCG
AciI 411 CCGC CCGC(-3/-1)
39 GCGG
AcsI 658 GAATTC [GA]^AATT[CT]
708 AAATTC
ApoI 658 GAATTC [GA]^AATT[CT]
708 AAATTC
BbvI 64 GCAGC GCAGC(8/12)
466 GCAGC
BseRI 18 CTCCTC GAGGAG(10/8)
128 GAGGAG
BsmAI 475 GAGAC GTCTC(1/5)
686 GAGAC
BsmI 117 GAATGC GAATGC(1/-1)
792 GAATGC
BstF5I 810 CATCC GGATG(2/0)
517 GGATG
BstNI 598 CCAGG CC^[AT]GG
813 CCTGG
BstUI 38 CGCG CG^CG
502 CGCG
BstYI 224 AGATCC [GA]^GATC[CT]
632 GGATCC
DdeI 23 CTGAG C^T[ACGT]AG
744 CTCAG
DsaI 250 CCACGG C^C[GA][CT]GG
722 CCATGG
EcoRII 598 CCAGG ^CC[AT]GG
813 CCTGG
EcoT14I 488 CCTTGG C^C[AT][AT]GG
722 CCATGG
FokI 810 CATCC GGATG(9/13)
517 GGATG
HapII 50 CCGG C^CGG
59 CCGG
HpaII 50 CCGG C^CGG
59 CCGG
HphI 259 GGTGA GGTGA(8/7)
291 GGTGA
MflI 224 AGATCC [GA]^GATC[CT]
632 GGATCC
MspI 50 CCGG C^CGG
59 CCGG
MvaI 598 CCAGG CC^[AT]GG
813 CCTGG
MvnI 38 CGCG CG^CG
502 CGCG
PleI 560 GACTC GAGTC(4/5)
688 GACTC
StyI 488 CCTTGG C^C[AT][AT]GG
722 CCATGG
TseI 64 GCAGC G^C[AT]GC
466 GCAGC
XhoII 224 AGATCC [GA]^GATC[CT]
632 GGATCC
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AluI AlwI BsaJI BsiYI BslI
BsrI BssKI DpnII Fnu4HI HaeIII
HinfI ItaI MaeIII MboI MnlI
MwoI NdeII NlaIII NlaIV Sau3AI
ScrFI TfiI Tsp45I Tsp509I TspRI